The Expression and Mechanism of Protease-activated Receptor 4 in Esophageal Cancer Cells

Jiang Ping; Li Shu De; Zhu Yue Chun; Li Si Man

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Jiang Ping , Li Shu De , Zhu Yue Chun , Li Si Man . The Expression and Mechanism of Protease-activated Receptor 4 in Esophageal Cancer Cells[J]. Journal of Kunming Medical University, 2017, 38(04): 14-17.

Citation:

Jiang Ping , Li Shu De , Zhu Yue Chun , Li Si Man . The Expression and Mechanism of Protease-activated Receptor 4 in Esophageal Cancer Cells[J]. Journal of Kunming Medical University, 2017, 38(04): 14-17.

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The Expression and Mechanism of Protease-activated Receptor 4 in Esophageal Cancer Cells

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2014FB002,2015FB103); 云南省教育厅科研基金资助项目(2014Y153,2016ZDX043);

Received Date: 2017-01-07

Abstract

Abstract

Objective To investigate the expression of PAR4 and mechanism of esophageal cancer cells.Methods(1) We used PCR and western blot to detect the expression of PAR4 in human esophageal epithelial cells(HEEpiC), human esophageal cancer cells(TE-10, TE-11,Eca-109).(2) The BSP method was used to test the methylation of PAR4 gene promoter region in four cells.Results(1) The expression level of PAR4 mRNA in TE-10,TE-11,Eca-109 cells was lower than that of HEEpiC cells.(2) In HEEpiC,TE-10,TE-11 and Eca-109,the frequency of methoylation occurred at the 19 CpG loci of PAR4 was 35.4%,83.8%,87.6% and94.3%, respectively. Conclusion The expression of PAR4 in esophageal cancer cells is increased, the hypermethylation at promoter of PAR4 gene may contribute to less expression of PAR4 in esophageal cancer cells.
- PAR4,
- Esophageal cancer cell,
- PCR,
- Western blot,
- Methylation

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References(13)

References

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