Wang Jun Min , Wu Xiao Hong , Wang Li , Lei De Hong , Qiu Liang Wu . Effects of Swimming Exercise on Serum IL-2 and IL-6 of Fetuin-A-induced Insulin Resistance Rats[J]. Journal of Kunming Medical University, 2017, 38(04): 18-21.
Citation: Wang Jun Min , Wu Xiao Hong , Wang Li , Lei De Hong , Qiu Liang Wu . Effects of Swimming Exercise on Serum IL-2 and IL-6 of Fetuin-A-induced Insulin Resistance Rats[J]. Journal of Kunming Medical University, 2017, 38(04): 18-21.

Effects of Swimming Exercise on Serum IL-2 and IL-6 of Fetuin-A-induced Insulin Resistance Rats

Funds:

基金: 福建省自然科学基金资助项目(2011J01151);

  • Received Date: 2016-09-13
  • Objective To explore the changes of serum IL-2 and IL-6 of rats with insulin resistance after swimming training intervention,so as to provide scientific basis for further reducing and preventing the occurrence and development of diabetes. Methods Eight weeks male SD rats were induced to insulin resistance model by abdominal injection of 0.75 mg/kg of bovine Fetuin-A(purity of 95%). The insulin resistance index(HOMA IR) 8hours after injection was detected to confirm the success of modeling. The model rats were randomly divided into control group(RR) and exercise group(RE),and normal rats were selected as the blank control group(group C), 6 rats in each group. Rats in the RE group were given swimming training without weight loading,60 min/d for8 weeks, rats in group C and group RR were removed from the swimming pool immediately after flooding.Immunohistochemistry and western blot method were used respectively to detect the serum Fetuin-A and the indexes of insulin resistance(HOMA IR). ELISA was used to detect serum IL-2, IL-2R and IL-6 levels. Results The HOMA-IR of rats in RE group was significantly lower than that in RR group. Compared with the RR group,the serum IL-2 levels of rats in RE group were increased significantly(P < 0.05). Compared with the C group,the serum IL-6 levels in RR group were increased significantly. Compared with the RR group,the serum IL-6levels in RE group were decreased significantly(P < 0.01). Conclusions Swimming training can significantly improve the IL-2 levels and the diastolic function of heart at the same time of reducing insulin resistance index. And the down-regulation of IL-6 expression suggests that IL-6 may also be used as a reference to test insulin resistance index.
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