Thermal Stability of an Antibacterial Metabolite from an Endophytic Penicillium griseofulvum in Paris Pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz

Wu Yi; Chen Fen Qing; Li Ya Jing; Yu Fang; Pan Hong Mei; Xuan Qun; Zhang Cai Jun; Zou Yu Song

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Wu Yi , Chen Fen Qing , Li Ya Jing , Yu Fang , Pan Hong Mei , Xuan Qun , Zhang Cai Jun , Zou Yu Song . Thermal Stability of an Antibacterial Metabolite from an Endophytic Penicillium griseofulvum in Paris Pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz[J]. Journal of Kunming Medical University, 2017, 38(07): 22-26.

Citation:

Wu Yi , Chen Fen Qing , Li Ya Jing , Yu Fang , Pan Hong Mei , Xuan Qun , Zhang Cai Jun , Zou Yu Song . Thermal Stability of an Antibacterial Metabolite from an Endophytic Penicillium griseofulvum in Paris Pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz[J]. Journal of Kunming Medical University, 2017, 38(07): 22-26.

Citation:

Wu Yi , Chen Fen Qing , Li Ya Jing , Yu Fang , Pan Hong Mei , Xuan Qun , Zhang Cai Jun , Zou Yu Song . Thermal Stability of an Antibacterial Metabolite from an Endophytic Penicillium griseofulvum in Paris Pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz[J]. Journal of Kunming Medical University, 2017, 38(07): 22-26.

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Thermal Stability of an Antibacterial Metabolite from an Endophytic Penicillium griseofulvum in Paris Pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz

Funds:

基金：云南省应用基础研究基金资助项目 (2014FB017); 昆明医科大学本科生创新研究基金资助项目 (J12101006);

Received Date: 2017-03-19

Abstract

Abstract

Objectives To study the component and thermal stability of the Antibacterial metabolite 108 from an fungal endophyte strain of Penicillium griseofulvum in Paris pohyphylla Smith var. yunnanensis (French.) Hand.-Mazz, and to compare its stability with that of amoxicillin. Methods The Staphylococcus aureus, Salmonella typhi and Endococcusfaecium were used as indicators to test the antibacterial activity of 108 in vitro (using paper disk test) . Then, thin layer chromatography (TLC) and column chromatography were used to separate and purify the secondary metabolites of P. griseofulvum. Finally, thermal stability of 108 was compared with amoxicillin's according to the diameters of inhibition zones. Results A compound with antibacterial activity named as 108 from metabolites of P. griseofulvum was isolated. The separated product 108 was found to be a single compound after ultraviolet scanning and thin layer chromatography. The diameters of inhibition zones against the indicators were 24 mm, 25.3mm and 7.6mm respectively at room temperature. Comparing with amoxicillin at the same temperature, this compound heated at 121.3℃ can keep steadier diameters of inhibition zones. Conclusion Compound 108, therefore, is considered to have tolerance of high pressure steam sterilization and strong thermal stability.

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References(23)

References

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