The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo

Yang Can; Gong Yu Hang; Wang Hai Feng; Li Hai Hao; Liu Jing Yu; Wang Wei; Wang Jian Song; Zuo Yi Gang; Chen Jian; Zhan Hui; Ding Ming Xia

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Yang Can , Gong Yu Hang , Wang Hai Feng , Li Hai Hao , Liu Jing Yu , Wang Wei , Wang Jian Song , Zuo Yi Gang , Chen Jian , Zhan Hui , Ding Ming Xia . The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo[J]. Journal of Kunming Medical University, 2018, 39(05): 1-6.

Citation:

Yang Can , Gong Yu Hang , Wang Hai Feng , Li Hai Hao , Liu Jing Yu , Wang Wei , Wang Jian Song , Zuo Yi Gang , Chen Jian , Zhan Hui , Ding Ming Xia . The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo[J]. Journal of Kunming Medical University, 2018, 39(05): 1-6.

Citation:

Yang Can , Gong Yu Hang , Wang Hai Feng , Li Hai Hao , Liu Jing Yu , Wang Wei , Wang Jian Song , Zuo Yi Gang , Chen Jian , Zhan Hui , Ding Ming Xia . The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo[J]. Journal of Kunming Medical University, 2018, 39(05): 1-6.

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The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo

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基金：国家自然科学基金资助项目 (81360376);

Received Date: 2017-09-20

Abstract

Abstract

Objective To research the monoclonal antibody KMP1 inhibited bladder cancer EJ cell lines growth and metastasis in vivo by bioluminescence imaging. Me thods Immunohistochemistry was used to determine the KMP1 binding to EJ and EJ-GFP cell lines. The xenograft tumor cell growth and distribution were measured by vernier calipers and dynamic in vivo fluorescence imaging. Immunohistochemistry and H&E counterstaining researched the feature of the xenograft tumor. Re s ults Cell growth curves of EJ and EJ-GFP cells were similar.EJ-GFP had a green fluorescence. In EJ-GFP nude mouse tumor model, the addition of KMP1 significantly inhibited tumor growth and extended the average life span of nude mice. Both EJ and EJ-GFP cells can bind to KMP1, and the weight of transplanted tumors in the KMP1 treatment group was significantly lower than that of the m Ig G control group (P <0.001) .Conclus ion KMP1 has a promising antitumor effect in vivo. It might be valuable for development as a promising targeted agent for bladder cancer.
- Bladder carcinoma,
- EJ cells,
- Monoclonal antibody

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References(14)

References

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