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Ye Lei , Tong Fa Chun , Li Jian , Wang Long , Zhao Jiang Ming , Liu Yi , Wang Jian . The Regulatory Effect of PPARy in the Apoptosis against Renal Cell Carcinoma[J]. Journal of Kunming Medical University, 2018, 39(05): 29-34.
Citation: Ye Lei , Tong Fa Chun , Li Jian , Wang Long , Zhao Jiang Ming , Liu Yi , Wang Jian . The Regulatory Effect of PPARy in the Apoptosis against Renal Cell Carcinoma[J]. Journal of Kunming Medical University, 2018, 39(05): 29-34.
shu

The Regulatory Effect of PPARy in the Apoptosis against Renal Cell Carcinoma

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基金： 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB082);

  • Received Date: 2017-11-19
    Abstract
  • Objective To explore the effects of PPARγ agonist rosiglitazone and inhibitor T0070907 on apoptosis and anti-tumor in renal carcinoma A498 cells.Me thods A498 cells were divided into three groups and PBS, rosiglitazone (50 μmol/L) and T0070907 50 (μmol/L) were added respectively of 24 h incubation completely. each group of cell proliferation was determined by MTT method and Western Blot analysis and RT-q PCR were applied to detect the expression level of BAX, Caspase 3, Cyt C and Bcl-2. A498 cell morphological changes were observed under light microscope and fluorescence microscope. Re s ults MTT experiment results showed that rosiglitazone and T0070907 could significantly inhibit A498 cell proliferation rate (P< 0.05) , increased the protein and m RNA expression levels of Caspase 3, Cyt C and Bax in A498 cell, and decreased the protein and m RNA expression levels of Bcl-2 (P <0.05) ; Microscopic observation and Hochest staining also found that rosiglitazone and T0070907 could promote apoptosis of A498 cells. Conclus ion Rosiglitazone and T0070907 can inhibit the proliferation of renal cell carcinoma A498 cells and induce apoptosis. The anti-tumor mechanism may be related to PPARγ mediation.
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