The Expression of LRG1 in Cervical Squamous Carcinoma and Its Relationship with Microvessel Density

Tian Rui; Duan Zhen Ling; Ma Jing

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Tian Rui , Duan Zhen Ling , Ma Jing . The Expression of LRG1 in Cervical Squamous Carcinoma and Its Relationship with Microvessel Density[J]. Journal of Kunming Medical University, 2018, 39(06): 44-51.

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Tian Rui , Duan Zhen Ling , Ma Jing . The Expression of LRG1 in Cervical Squamous Carcinoma and Its Relationship with Microvessel Density[J]. Journal of Kunming Medical University, 2018, 39(06): 44-51.

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The Expression of LRG1 in Cervical Squamous Carcinoma and Its Relationship with Microvessel Density

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基金：国家自然科学基金资助项目 (81460240);

Received Date: 2018-02-11

Abstract

Abstract

Objective To investigate the expression of LRG1 (Leucine-rich alpha-2-glycoprotein 1) and CD105 (Endoglin) in normal cervical tissues, cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ and cervical carcinoma. Furthermore, to explore the function of LRG1 in cervical carcinoma pathogenesis and the relationship between LRG1 and microvessel density.Me thods The expression levels of LRG1 and CD105 were detected by immunohistochemistry in 40 cervical carcinoma tissues, 20 CIN Ⅱ-Ⅲ tissues and 20 normal cervical tissues.Re s ults Compared to the normal cervix, the expression of LRG1 in CIN Ⅱ-Ⅲ and cervical squamous cell carcinoma was significantly increased (P<0.05) .LRG1 expression was correlated to clinical stage and lymph node metastasis (P <0.05) . But there was no correlation between LRG1 expression and age, the size of tumor, pathologic grades and depth of invasion (P > 0.05) .With the deepening of cervical lesions, CD105-MVD (X±s) increasedsignificantly (P<0.05) .The expression of CD105-MVD in cervical carcinomawas related to clinical stage, lymph node metastasis and the size of tumor. The difference was statistically significant (P<0.05) . There was no correlation between CD105-MVD and age, pathologic grades and depth of invasion (P > 0.05) .Positive correlation was found between the expression of LRG1 and CD105-MVD (r = 0.944, P <0.05) .Conclus ions The expression of LRG1 and CD105-MVD is up-regulated and shows a positive correlation, which suggests that the abnormal expression of LRG1 and CD105-MVD may involve in the occurrence and development of cervical squamous carcinoma.
- LRG1,
- CD105,
- MVD,
- Cervical squamouscarcinoma

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References(24)

References

[1]	[1]FERLAY J, SOERJOMATARAM I, DIKSHIT R, et al.Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012[J].International Journal of Cancer, 2015, 136 (6) :359-386.
[2]	[2]DI ZHONG, SIREN ZHAO, GUANGXU HE, et al.Stable knockdown of LRG1 by RNA interference inhibits growth and promotes apoptosis of glioblastoma cells in vitro and in vivo[J].Tumor Biol, 2015, 36 (6) :4271-4278.
[3]	[3]SHAN-YUN WEN, LI-NA ZHANG, XIAO-MEI YANG, et al.LRG1 is an independent prognostic factor for endometrial carcinoma[J].Tumor Biol, 2014, 35 (7) :7125-7133.
[4]	[4]ANDERSEN J D, BOYLAN K L, JEMMERSON R, et al.Leucine-rich alpha-2-glycoprotein-1 is upregulated in sera and tumors of ovarian cancer patients[J].Ovarian Res, 2010, 3:21.
[5]	[5]ZHANG J, ZHU L, FANG J, et al.LRG1 modulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via HIF-1alpha activation[J].J Exp Clin Cancer Res, 2016, 35 (1) :29.
[6]	[6]KOICHI SAITO, TOSHIYUKI TANAKA, HIDENOBU KANDA, et al.Gene expression profiling of mucosal addressin cell adhesion molecule-1+high Endothelial venule cells (HEV) and identification of a leucine-rich HEV Glycoprotein as a HEV marker[J].J Immunol, 2002, 168 (3) :1050-1059.
[7]	[7]WANG X, ABRAHAM S, MCKENZIE JA, et al.Lrg1 promotes angiogenesis by modulating endothelial TGFβsignaling[J].Nature, 2013, 499 (7458) :306-311.
[8]	[8]MASSAGU J.TGFbeta in cancer[J].Cell, 2008, 134 (2) :215-230.
[9]龙瑞清, 刘卓慧, 张帆, 等.绿脓假单胞菌感染中耳上皮细胞可以激活TGF-β1 Smad信号通路[J].昆明医科大学学报, 2017, 38 (10) :65-69.
[10]	[10]FLEMING N I, JORISSEN R N, MOURADOV D, et al.SMAD2, SMAD3 and SMAD4 mutations in colorectal cancer[J].Cancer Res, 2013, 73 (2) :725-735.
[11]	[11]CHEN R, XIE M L.The research progress of TGF-β/Smads signal pathway in the treatment of myocardial fibrosis and its application[J].Chin Pharmacol Bull, 2012, 28 (9) :1189-1192.
[12]雷雯, 张涛, 赵晓远, 等.IL-27调控TGF-β/Smad通路在博来霉素诱导肺纤维化中的作用[J].昆明医科大学学报, 2016, 37 (5) :9-12.
[13]	[13]LYNCH J, FAY J, MEEHAN M, et al.Stallings RL.Mirna-335 suppresses neuroblastoma cell invasiveness by direct targeting of multiple genes from the non-canonical TGF-βsignaling pathway[J].Carcinogenesis, 2012, 33 (5) :976-985.
[14]	[14]HAUPT H, BAUDNER S.Isolation and characterization of an unknown, leucine-rich 3.1-S-alpha2-glycoprotein from human serum[J].Hoppe Seylers Z Physiol Chem, 1977, 358 (6) :639-646.
[15]	[15]CODINA R, VANASSE A, KELEKAR A, et al.Cytochrome c-induced lymphocyte death from the outside in:inhibition by serum leucine-rich alpha-2-glycoprotein-1[J].Apoptosis, 2010, 15 (2) :139-152.
[16]	[16]TEN DIJKE P, ARTHUR H M.Extracellular control of TGF-beta signalling in vascular development and disease[J].Nat Rev Mol Cell Biol, 2007, 8 (11) :857-869.
[17]	[17]OTTONELLO L, GHIO M, CONTINI P, et al.Nonleukoreduced red blood cell transfusion induces a sustained inhibition of neutrophil chemotaxis by stimulating in vivo production of transforming growth factor-beta 1 by neutrophils:role of the immunoglobulin like transcript 1, s Fas L, and s HLA-I[J].Transfusion, 2007, 47 (8) :1395-1404.
[18]	[18]VAN DEN DRIESCHE S, MUMMERY C L, WESTERMANN C J.Hereditary hemorrhagic telangiectasia:an update on transforming growth factor beta signaling invasculogenesis and angiogenesis[J].Cardiovasc Res, 2003, 58 (1) :20-31.
[19]	[19]TOPORSIAN M, GROS R, KABIR MG, et al.A role for endoglin in coupling e NOS activity and regulating vascular tone revealed in hereditary hemorrhagic telangiectasia[J].Circ Res, 2005, 96 (6) :684-692.
[20]	[20]FOLKMAN J.Tumor angiogenesis:therapeutic implications[J].N Engl J Med, 1971, 285 (21) :1182-1186.
[21]何迷, 王家平, 张磊, 等.多西紫杉醇在肝癌细胞裸鼠肝脏移植瘤中对P53、VEGF作用的研究[J].昆明医科大学学报, 2015, 36 (1) :43-47.
[22]	[22]SAAD R S, JASNOSZ K M, TUNG MY, et al.Endoglin (CD105) expression in endometrial carcinoma[J].Int J Gynecol Pathol, 2003, 22 (3) :248-253.
[23]	[23]DALLAS N A, SAMUEL S, XIA L, et al.Endoglin (CD105) :a marker of tumor vasculature and potential target for therapy[J].Clin Cancer Res, 2008, 14 (7) :1931-1937.
[24]	[24]STEPAN D, SIMIONESCU C, STEPAN A, et al.VEGF and CD105 immunoexpression in squamous cervical carcinomas and associated precancerous lesions[J].Rom J Morphol Embryol, 2012, 53 (3) :585-589.

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