Effects of Rutin on Proliferation of Keloid Fibroblasts

Li Yu Peng; Jiao Jian Lin; Lv Le Chun

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(07): 1-4

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Citation:

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Citation:

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

PDF( 1252 KB)

Effects of Rutin on Proliferation of Keloid Fibroblasts

Funds:

基金：国家自然科学基金资助项目 (81560502); 云南省自然科学基金资助项目 (2017FB116); 昆明医科大学“百名中青年学术和技术骨干”资助项目;

Received Date: 2018-02-13

Abstract

Abstract

Objective To investigate the effects of rutin on the proliferation of keloid fibroblasts.Methods Keloid tissue was used to induce fibroblasts and 5 concentration gradients of rutin were set: 0, 50, 100, 200 and300 μmol/L.The effects of rutin on the proliferation of keloid-derived fibroblasts were observed by CCK-8 and Ed U assays.Western blot was applied to detect the effect of rutin on TGF-β/Smad signaling pathway.Re s ults Rutin inhibited the proliferation of keloid-derived fibroblasts in a dose-dependent manner when concentration gradient was lower than 300 μmol/L (P<0.01) . The result of Ed U assay showed that the synthesis of DNA in keloid-derived fibroblasts was inhibited by rutin. At the concentration gradient of 50, 100 and 200 μmol/L, rutin could significantly inhibit the expression of TGF-β1 and Smad2 in keloid-derived fibroblasts.Conclus ion Rutin inhibits the proliferation of keloid-derived fibroblasts by regulating TGF-β/Smad signaling pathway.
- Keloid,
- Fibroblast,
- Rutin,
- Proliferation

FullText(HTML)

References(8)

References

[1]	[1]LI Y, ZHANG J, LEI Y, et al.Micro RNA-21 in Skin Fibrosis:Potential for Diagnosis and Treatment[J].Mol Diagn Ther, 2017, 21 (6) :633-642.
[2]	[2]ZHANG J, XU D, LI N, et al.Downregulation of micro RNA-31 inhibits proliferation and induces apoptosis by targeting HIF1AN in human keloid[J].Oncotarget, 2017, 8 (43) :74623-74634.
[3]伍庆, 王兴宁, 张明时, 等.高效液相色谱法同时测定杜仲叶药材中芦丁等3种黄酮类有效成分的含量[J].时珍国医国药, 2009, 20 (5) :1186-1187.
[4]	[4]GEGOTEK A, BIELAWSKA K, BIERNACKI M, et al.Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation[J].Redox Biol, 2017, 12 (C) :733-744.
[5]	[5]WU F, CHEN J, FAN LM, et al.Analysis of the effect of rutin on GSK-3βand TNF-αexpression in lung cancer[J].Exp Ther Med, 2017, 14 (1) :127-130.
[6]沈钦海, 马臻, 陈国民.芦丁对Hep G2细胞生长的影响[J].第三军医大学学报, 2006, 28 (18) :1885-1887.
[7]	[7]LAKSHMI T, EZHILARASAN D, NAGAICH U, et al.Acacia catechu ethanolic seed extract triggers apoptosis of SCC-25 cells[J].Pharmacogn Mag, 2017, 13 (Suppl 3) :S405-S411.
[8]	[8]HE Y, DENG Z, ALGHAMDI M, et al.From genetics to epigenetics:new insights into keloid scarring[J].Cell Prolif, 2017, 50 (2) :e12326.

Relative Articles(20)

Relative Articles

[1]	Yichao JIN, Xiaochuan WANG, Chunping AO, Liangheng XU. Pruritus in Keloid：Pathogenesis and Treatments. Journal of Kunming Medical University, 2025, 46(5): 1-5.
[2]	Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
[3]	Yuru ZHAI, Yan BAI, Yunyun LI. FGF2 Regulates Hypoxia-Induced Proliferation and Collagen Metabolism of Scleral Fibroblasts Through the PERK/EIF2α/ATF4 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(10): 22-28. doi: 10.12259/j.issn.2095-610X.S20241004
[4]	Shiyuan YANG, Hao ZHANG, Tuqiang HU. Effect of Pristimerin on Proliferation of Oral Squamous Cell Carcinoma CAL-27 by Regulating Autophagy. Journal of Kunming Medical University, 2023, 44(10): 92-99. doi: 10.12259/j.issn.2095-610X.S20231024
[5]	Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028
[6]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[7]	Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731
[8]	Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223
[9]	Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29. doi: 10.12259/j.issn.2095-610X.S20221206
[10]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[11]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[12]	Wang Jun, Zhang Yi , Zhang Shi Hui , Jin Fu Yu , Li Tian , Xu Hong . . Journal of Kunming Medical University, 2020, 41(04): 6-10.
[13]	Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
[14]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[15]	Ni Tao . . Journal of Kunming Medical University,
[16]	Wang Hai Feng . . Journal of Kunming Medical University,
[17]	Liu Jia Xin . . Journal of Kunming Medical University,
[18]	Wu Bin . . Journal of Kunming Medical University,
[19]	Xia Ying Jie . . Journal of Kunming Medical University,
[20]	Li Xiong . . Journal of Kunming Medical University,