Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960

Li Xiao Qin; Zhou Min; Wang Lin; Zeng Hai Yan; Luo Peng Ju; Li Ying; Ran Li Quan

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Article Navigation > Journal of Kunming Medical University > 2019 > 40(03): 112-115

Li Xiao Qin , Zhou Min , Wang Lin , Zeng Hai Yan , Luo Peng Ju , Li Ying , Ran Li Quan . Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960[J]. Journal of Kunming Medical University, 2019, 40(03): 112-115.

Citation:

Li Xiao Qin , Zhou Min , Wang Lin , Zeng Hai Yan , Luo Peng Ju , Li Ying , Ran Li Quan . Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960[J]. Journal of Kunming Medical University, 2019, 40(03): 112-115.

Citation:

Li Xiao Qin , Zhou Min , Wang Lin , Zeng Hai Yan , Luo Peng Ju , Li Ying , Ran Li Quan . Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960[J]. Journal of Kunming Medical University, 2019, 40(03): 112-115.

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Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960

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基金：昆明市卫生和计划生育委员会医药卫生科技计划项目 (2018-03-02-001);

Received Date: 2018-10-21

Abstract

Abstract

Objective To analyze and compare rifampicin-resistant gene mutation detection and BACTEC MGIT 960 system phenotype susceptibility test in Mycobacterium tuberculosis by PCR-reverse dot blot hybridization.Methods At the same time, the phenotypic susceptibility of rifampicin to rifampicin was detected by PCR-reverse dot blot hybridization in sputum or bronchoalveolar lavage fluid (BALF) from 220 inpatients and by BACTEC MGIT 960 System. Results The susceptibility of rifampicin to rifampicin by the two methods accorded with 205 cases and the specificity was 93% (205/220) ; both methods were sensitive to rifampicin in 172 cases; gene resistance and phenotypic resistance in 33 cases, of which 3 cases detected mutation at the H526 locus of rpo B gene, with a mutation rate of 9%; gene resistance and phenotypic sensitivity in 15 cases, 11 of which were all detected. The mutation of the H526 locus of rpo B gene was 73%.Conclusions PCR-reverse dot hybridization has the characteristics of high speed, high efficiency and specificity. It has guiding significance for clinical diagnosis and treatment.
- M.tuberculosis,
- Rifampicin,
- Gene analysis on drug-resistant tuberculosis

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References(14)

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