Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells

Hu Tao; Liu Guang Cai; Hong Ying; Chen Hong Lan; Chang Ye Fei; Jiang Shui; Tan Ya Xin; Xi Yang Yan Bin; Chen Bo

Article Contents

Article Navigation > Journal of Kunming Medical University > 2019 > 40(03): 6-10

Hu Tao , Liu Guang Cai , Hong Ying , Chen Hong Lan , Chang Ye Fei , Jiang Shui , Tan Ya Xin , Xi Yang Yan Bin , Chen Bo . Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2019, 40(03): 6-10.

Citation:

Hu Tao , Liu Guang Cai , Hong Ying , Chen Hong Lan , Chang Ye Fei , Jiang Shui , Tan Ya Xin , Xi Yang Yan Bin , Chen Bo . Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2019, 40(03): 6-10.

Citation:

Hu Tao , Liu Guang Cai , Hong Ying , Chen Hong Lan , Chang Ye Fei , Jiang Shui , Tan Ya Xin , Xi Yang Yan Bin , Chen Bo . Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2019, 40(03): 6-10.

PDF( 1430 KB)

Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells

Funds:

基金：国家自然科学基金资助项目 (81502377); 云南省应用基础研究计划资助项目 (2016FB139); 云南省教育厅科学研究基金资助项目 (2016ZDX044);

Received Date: 2018-07-14

Abstract

Abstract

Objective To explore the expression changes of miRNAs in HRHPV (16 and 18) infected (+) cervical cancer cells after G6 PD expression knock-down. Methods The G6 PD expression in HPV-C33 A, HPV16+Siha and HPV18+Hela cells was inhibited by RNA interference target G6 PD. Human micro RNA Microarray Kit and q RT-PCR were employed to determine the expression changes of mi RNA between different cell lines.Results The expression of G6 PD significantly decreased after RNA interference in different cervical cancer cell lines.Mi RNA microarrayand q RT-PCR analysis revealed that numerousmiRNAs in HPV+ Hela/Siha cells were expressional different from the control HPV-C33 A cells, following G6 PD knockdown. Conclusion The expression changes of various miRNAs following G6 PDknockdown are associated with the oncogenesis events of G6 PD in HR HPV infected cervical cancer cells.
- Cervical cancer,
- High-risk human papillomavirus (HR HPV) 16/18 infection,
- G6PD,
- miRNAs

FullText(HTML)

References(24)

References

[1]	[1] JEMAL A, BRAY F, CENTER M M, et al. Global cancer statistics[J]. CA Cancer J Clin, 2011, 61 (2) :69-90.
[2]	[2]ZUR HAUSEN H. Papillomaviruses and cancer:from basic studies to clinical application[J]. Nat Rev Cancer, 2002, 2 (5) :342-350.
[3]	[3]WALBOOMERS J M, JACOBS M V, MANOS M M, et al.Human papillomavirus is a necessary cause of invasive cervical cancer worldwide[J].J Pathol, 1999, 189 (1) :12-19.
[4]	[4]HU T, LI Y S, CHEN B, et al.Elevated glucose-6-phosphate dehydrogenase expression in the cervical cancer cases is associated with the cancerigenic event of high-risk human papillomaviruses[J]. Exp Biol Med (Maywood) , 2015, 240 (10) :1287-1297.
[5]	[5]HU T, ZHANG C, TANG Q, et al. Variant G6PD levels promote tumor cell proliferation or apoptosis via the STAT3/5 pathway in the human melanoma xenograft mouse model[J]. BMC Cancer, 2013, 13:251.
[6]	[6]ZHANG M, DONG B B, LU M, et al. mi R-429 functions as a tumor suppressor by targeting FSCN1 in gastric cancer cells[J]. Onco Targets Ther, 2016, 9:1123-1133.
[7]李瑞岩, 蒋传路.脑胶质瘤中G6PD蛋白的表达及与肿瘤分级的相关性[J].黑龙江医学, 2008, 32 (8) :573-575.
[8]	[8]KEKEC Y, PAYDAS S, TULI A, et al. Antioxidant enzyme levels in cases with gastrointesinal cancer[J]. Eur J Intern Med, 2009, 20 (4) :403-406.
[9]	[9]LANGBEIN S, FREDERIKS W M, ZUR HAUSEN A, et al.Metastasis is promoted by a bioenergetic switch:New targets for progressive renal cell cancer[J]. Cancer, 2008, 12 (2) :2422-2428.
[10]	[10]GERACI G, D'ELIA I, GAUDIO R. Evidence of genetic instability in tumors and normal nearby tissues[J]. PLo S ONE, 2010, 5 (2) :e9343.
[11]	[11]KOUDSTAAL J, MAKKINK B, OVERDIEP S H. Enzyme histochemical pattern in human tumours. II. Oxidoreductases in carcinoma of the colon and the breast[J]. Eur J Cancer, 1975, 11 (2) :111-115.
[12]	[12]BAHA M, YAMAOTO R, IISHI H, et al. Role of glucose-6-phosphate dehydrogenase on enhanced proliferation of pre-neoplastic and neoplastic cells in rat liver induced by N-nitrosomorpholine[J]. Int J Cancer, 1989, 43 (5) :892-895.
[13]	[13]HU T, CHANG YF, XIAO Z, et al. mi R-1 inhibits progression of high-risk papillomavirus-associated human cervical cancer by targeting G6PD[J].Oncotarget, 2016, 7 (52) :86103-86116.
[14]	[14]JOHN B, EERIGHT AJ, ARAVIN A, et al. Human MicroRNA targets[J]. PLo S Biol, 2004, 2 (11) :e363.
[15]	[15]SATOH J. Molecular network analysis of human micro RNA targetome:from cancers to Alzheimer’s disease[J]. Bio Data Min, 2012, 5 (1) :17.
[16]	[16]FUJII R, OSAKA E, SATO K, TOKUHASHI Y, et al.Mi R-1 Suppresses Proliferation of Osteosarcoma Cells by Up-regulating p21 via PAX3[J].Cancer Genomics Proteomics, 2019, 16 (1) :71-79.
[17]	[17]DU YY, ZHAO LM, CHEN L, et al. The tumor-suppressive function of mi R-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma[J]. J GastroenterolHepatol, 2015, 31 (2) :384-393.
[18]	[18]XU X, WU X, JIANG Q, et al. Downregulation of micro RNA-1 and micro RNA-145 contributes synergistically to the development of colon cancer[J]. Int J Mol Med, 2015, 36 (6) :1630-1638.
[19]	[19]KORI M, YALCIN ARGA K.Potential biomarkers and therapeutic targets in cervical cancer:Insights from the meta-analysis of transcriptomics data within network biomedicine perspective[J].PLo S One, 2018, 13 (7) :e0200717.
[20]	[20]LIANG H, LI Y, LUO RY, et al. MicroRNA-215 is a potential prognostic marker for cervical cancer[J]. J Huazhong Univ Sc i Technolog Med Sci, 2014, 34 (2) :207-212.
[21]	[21] LIANG H, LI Y, LUO RY, et al.MicroRNA-215 is a potential prognostic marker for cervical cancer[J].J Huazhong Univ Sci Technolog Med Sci, 2014, 34 (2) :207-212.
[22]	[22]SONG R, CONG L, NI G, et al.MicroRNA-195 inhibits the behavior of cervical cancer tumors by directly targeting HDGF[J].Oncol Lett, 2017, 14 (1) :767-775.
[23]	[23]WANG R, ZHAO N, LI S, et al. MicroRNA-195 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting the expression of VEGF, VAV2, and CDC42[J]. Hepatology, 2013, 58 (2) :642-653.
[24]	[24]ZHOU Q, HAN LR, ZHOU YX, et al.MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3[J].Int J Gynecol Cancer, 2016, 26 (5) :817-824.

Relative Articles(20)

Relative Articles

[1]	Xiangjie CUI, Yufen TAO, Lanfang ZHU, Yufeng YAO, Li SHI. Evaluations of Immunogenicity and Efficacy of A Novel HPV16 E6 and E7 Multi-epitope DNA Vaccine. Journal of Kunming Medical University, 2024, 45(5): 16-22. doi: 10.12259/j.issn.2095-610X.S20240503
[2]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[3]	Ni GUO, Cheng ZHANG, Chao HONG, Weipeng LIU, Yufeng YAO, Zhiling YAN. Correlation of KRAS Gene 3′ UTR Polymorphisms with Cervical Cancer and Cervical Intraepithelial Neoplasia in Chinese Han Population in Yunnan Province. Journal of Kunming Medical University, 2024, 45(2): 14-22. doi: 10.12259/j.issn.2095-610X.S20240203
[4]	Chunxia MIAO, Yina WANG, Yunxia ZHANG. Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis. Journal of Kunming Medical University, 2024, 45(7): 62-68. doi: 10.12259/j.issn.2095-610X.S20240709
[5]	Jinbao XIAO, Junda ZHAO, Junqi MA. Metabolomics of Siha Supernatant in Cervical Cancer Cells with Down-regulated HPV16 E6/E7 Expression. Journal of Kunming Medical University, 2024, 45(1): 22-27. doi: 10.12259/j.issn.2095-610X.S20240104
[6]	Qian LIU, Tuerxun HATILA, Hasimu AXIANGU. HLA-G Enhances the Killing Effect of NK Cells on Cervical Cancer Cells by Promoting the Expression of Activated Receptor. Journal of Kunming Medical University, 2022, 43(7): 46-54. doi: 10.12259/j.issn.2095-610X.S20220714
[7]	Honglan CHEN, Yi WU, Tao HU, Yuming WANG. Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection. Journal of Kunming Medical University, 2022, 43(2): 89-95. doi: 10.12259/j.issn.2095-610X.S20220203
[8]	Juan ZHANG, Hong-yun ZHANG. The Value of Endocervical Curettage in Cervical Cancer Screening. Journal of Kunming Medical University, 2021, 42(8): 123-127. doi: 10.12259/j.issn.2095-610X.S20210822
[9]	Zi-hong CHEN, Hong LIU, Ming-wei LIU, Mi SHU, Qiong NAN. Research Progress of miRNA-145 in Malignant Tumors of Digestive System. Journal of Kunming Medical University, 2021, 42(3): 145-148. doi: 10.12259/j.issn.2095-610X.S20210334
[10]	Ding-ji TAN, Hong-li YIN, Rui ZHU, Xi ZHANG, Xin YU, Yong FEI, Xin LI, Ming DUAN, Liang HE, Hong-ying YANG. Analysis of Vaginal Microecological in Women with Cervical Intraepithelial Neoplasia and Cervical Cancer. Journal of Kunming Medical University, 2021, 42(8): 118-122. doi: 10.12259/j.issn.2095-610X.S20210821
[11]	Qing Qing , Liu Yang , Zhou Hong Lin . . Journal of Kunming Medical University, 2020, 41(06): 162-166.
[12]	Hu Tao , Hong Ying , Chen Hong Lan , Chang Ye Fei , Jiang Shui , Xi Yang Yan Bin , Tan Ya Xin , Li Shan , Liu Guang Cai , Wu Xiao Hong . . Journal of Kunming Medical University, 2018, 39(12): 11-15.
[13]	Li Ting , Bian Li . Related Research and Development MicroRNAs in Lung Cancer. Journal of Kunming Medical University, 2017, 38(07): 130-135.
[14]	Li Zheng Jin . Application of 13 High-risk HPV infection Test Combined with Thinprep Cytologic Test on Screening Cervical Carcinoma in Dali Region， Yunnan Province. Journal of Kunming Medical University,
[15]	Wei Jie . . Journal of Kunming Medical University,
[16]	Li Zheng . . Journal of Kunming Medical University,
[17]	Zhao Dong Yan . . Journal of Kunming Medical University,
[18]	Yan Zhi Ling . . Journal of Kunming Medical University,
[19]	Liu Hui Jin . . Journal of Kunming Medical University,
[20]	Huang Ya . . Journal of Kunming Medical University,