Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols

Xin WANG; Yubo WEI; Yupeng LI; Jiacan YAO; Fang YU

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Xin WANG, Yubo WEI, Yupeng LI, Jiacan YAO, Fang YU. Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols[J]. Journal of Kunming Medical University.

Citation:

Xin WANG, Yubo WEI, Yupeng LI, Jiacan YAO, Fang YU. Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols[J]. Journal of Kunming Medical University.

Xin WANG, Yubo WEI, Yupeng LI, Jiacan YAO, Fang YU. Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols[J]. Journal of Kunming Medical University.

Citation:

Xin WANG, Yubo WEI, Yupeng LI, Jiacan YAO, Fang YU. Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols[J]. Journal of Kunming Medical University.

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Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols

Xin WANG^{1, 2},
Yubo WEI^{1, 2},
Yupeng LI^{1, 2},
Jiacan YAO^{1, 2
,
,},
Fang YU^{1, 2
,
,}

1.
College of Pharmacy & Key Laboratory of Pharmacology for Natural Products of Yunnan Province
2.
The College of Modern Biomedical Industry，Kunming Medical University，Kunming Yunnan 650500，China

Abstract

Abstract

Objective To develop a method capable of real-time, rapid, and visual detection of MEs , providing a powerful tool for early diagnosis and assessment of diseases. Methods In this study, flake graphene oxide （2D GO） with excellent peroxidase-like activity （POD-like） was prepared by a modified Hummers' method, and a sensing strategy for the rapid visual detection of MEs was constructed by integrating colorimetry with a smartphone. Results 2D GO can catalyse the decomposition of hydrogen peroxide （H₂O₂） to produce highly oxidative hydroxyl radicals （·OH）, which oxidize the colourless 3, 3', 5, 5'-tetramethylbenzidine （TMB） into blue ox-TMB. The strong reducing effect of MEs on the "2D GO + TMB + H2O2" colorimetric sensing system enables rapid visual detection of MEs. Conclusion The colorimetric platform constructed based on 2D GO has a wide linear detection range （10-1000 μmol/L） and a good detection limit （LOD < 7 μM）, and was successfully used for the determination of MEs in foetal bovine serum samples, with satisfactory recovery rates.
- Graphene oxide,
- Biothiols,
- Colorimetric sensing,
- Peroxide mimics enzymes

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References(29)

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