全自动酶联免疫分析仪与手工酶联免疫法的检测结果比较
Comparison of the Test Results between Automatic Enzyme-linked Immunity Analyzer and Manual Enzyme-linked Immunoassay
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摘要: ［摘要］目的 比对全自动酶联免疫分析仪与手工酶联免疫法检测临床标本结果的相关性及符合率，探讨更科学可靠的检验方法．方法 随机抽取90份临床与体检标本，经Tecan Freedom Evolyzer-2200全自动酶联免疫分析仪和手工加样操作的IMARK2酶标仪测定甲肝、乙肝5项、丙肝、戊肝2项、TP及HIV的结果，并对结果进行相关性及符合率的分析．结果 对所检测项目中的HBsAg、HBsAb、HBeAg、TP、HIV的相关性系数r2＞0.9 ，其数据显示相关性较好；而HAV-IgM、HBeAb、HBcAb、HCV、HEV-IgM、HEV-IgG、的相关性系数为 0.9＞r2＞0.6 ；Tecan Freedom Evolyzer-2200全自动酶联免疫分析仪与手工酶联免疫法操作比对90个样本的11项检测结果总符合率达96.9％． 结论 全自动酶联免疫分析仪与手工酶联免疫法有较好的相关性．全自动酶联免疫分析仪的使用还大大的减轻了实验室人员的劳动负担，提高工作效率，应加强临床推广．
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关键词:
- [关键词]全自动酶联免疫分析仪 /
- 手工酶联免疫法 /
- ELISA的影响因素
Abstract: ［Abstract］Objective To compare the consistency of the results of clinical specimens between Tecan Freedom Evolyzer-2200 automated enzyme immunoassay analyzer and manual ELISA method， so as to explore more scientific and reliable test method. Methods Ninety samples were randomly selected from clinical and physical examination，the IMARK2 microplate reader and the instrument operating manual sample measurement was used to test hepatitis A，hepatitis B 5， hepatitis C，hepatitis E 2，TP and HIV，and the results were given correlation and linear regression analysis. Results For the test items of HBsAg，HBsAb， HBeAg， TP， HIV， the correlation coefficient was r2> 0.9，the data showed a good correlation，and for HAV-IgM， HBeAb， HBcAb， HCV，HEV-IgM，HEV-IgG，the correlation coefficient was 0.9> r 2 > 0.6. Compared with manual method， the total coincidence rate of automated enzyme immunoassay analyzer was 96.9% in 11 indexes of 90 samples. Conclusions Automatic and manual ELISA method has a good correlation. Automatic ELISA also greatly reduce the labor burden of laboratory personnel， improve efficiency， should be strengthened in clinical practice. -
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