Predictive Value of Peripheral Blood Septin9 Gene Methylation Assay in Colorectal Adenoma
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摘要:
目的 探讨外周血Septin9基因甲基化（mSEPT9）检测在结直肠腺瘤诊断中的预测意义。 方法 收集2020年10月至2022年5月在昆明市第一人民医院病理科诊断为结直肠腺瘤的31名患者作为实验组，21例肠镜阴性受试者（消化科门诊患者）作为对照组。对2组人员进行外周血mSEPT9检测，并收集其相应外周血CEA检测结果，对检测结果采用受试者特征曲线进行统计分析。 结果 mSEPT9检测对腺瘤的曲线下预测面积（AREA of the ROC：AUC）为0.7205（P < 0.05），分界值（CT值）为39.55，此时对应的敏感度为90.91%，特异度为56.67%；CEA检测对腺瘤的AUC为0.5333（P > 0.05）。 结论 外周血mSEPT9检测筛查结直肠腺瘤效果优于外周血CEA肿瘤标记物，具有较好的敏感度及特异度，一定程度上对mSEPT9筛查阳性人群再行侵入性肠镜检查，更易为该类人群接受且可早期筛查结直肠腺瘤。 Abstract:Objective To investigate the predictive significance of Septin9 gene methylation（mSEPT9） in peripheral blood in the diagnosis of colorectal adenoma. Methods A total of 52 subjects were included, 31 patients with colorectal adenomas were collected as the experimental group in the Department of Pathology and 21 subjects with negative colonoscopy in the gastroenterology outpatient clinic were used as the control group from October 2020 to May 2022. mSEPT9 was detected in the two groups, and the results of CEA level in peripheral blood were collected. All the results were statistically analyzed using the Receiver Operating Characteristic（ROC）. Results The area under the curve（area of the ROC: AUC）of mSEPT9detectionto predict the development of adenoma was 0.7205（P < 0.05）, And the cut-off value（CT value） was 39.55, the corresponding sensitivity was 90.91% and the specificity was 56.67%. The AUC of CEA detection for predicting adenoma was 0.5333（P > 0.05）. Conclusions The detection of mSEPT9 is better than that of CEA tumor marker detection in peripheral blood for screening colorectal adenomas with good sensitivity and relative specificity. Invasive colonoscopy for people with positive mSEPT9 screening results can be easier to accept by the general population. -
Key words:
- Septin9 Gene /
- Methylation detection /
- Colorectal adenoma
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图 1 结直肠低、高级别腺瘤的HE病理形态（×20）
A：低级别管状腺瘤；B：低级别绒毛状管状腺瘤； C：高级别绒毛管状腺瘤；D：高级别锯齿状腺瘤。
Figure 1. Pathological morphology of colorectal low- and high-grade adenoma by HE staining （×20）
图 2 外周血mSEPT9检测结果与CEA血清浓度的ROC曲线比较
A：外周血mSEPT9甲基化ROC曲线；B：血清CEA表达水平ROC曲线。
Figure 2. The ROC comparison between the detection of mSEPT9 and CEA in peripheral blood
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