The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits
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摘要:
目的 探究明胶海绵微粒化疗联合肝动脉化疗栓塞对兔肝移植瘤瘤体生长的影响。 方法 60只新西兰大白兔随机分为模型对照组、明胶海绵微粒（gelatin sponge particles，GSPs）-肝动脉化疗栓塞（Transcatheterarterial chemoembolization，TACE）组、表柔比星-TACE组及GSPs+表柔比星-TACE组（n = 15）。所有实验兔均建立VX2细胞肝癌模型。术后，GSPs-TACE组TACE术中灌注GSPs，表柔比星-TACE组以表柔比星溶液（1mg/mL）灌注，GSPs+表柔比星-TACE组以表柔比星溶液混合 GSPs栓塞。计算机断层扫描检测各组实验兔肿瘤体积；苏木精-伊红染色观察病理形态；末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（Terminal Deoxynucleotidyl Transferase-Mediated dUTPNick End Labeling，TUNEL）染色检测肿瘤细胞凋亡率；免疫组化检测血管内皮生长因子（vascular endothelial growth factor，VEGF）阳性表达；免疫印迹检测B细胞淋巴瘤-2（B-cell lymphoma 2，Bcl-2）、Bcl-2关联X蛋白（Bcl-2-associated X protein，Bax）及半胱氨酸天冬氨酸蛋白酶-3（cysteine-aspartic acid protease-3，caspase-3）蛋白表达。 结果 与模型对照组相比，GSPs-TACE组肿瘤体积、肝内转移占比、腹腔内转移占比、VEGF、Bcl-2均降低（P < 0.05），肿瘤细胞凋亡率、Bax、Bcl-2/Bax、caspase-3均升高（P < 0.05）；GSPs-TACE组与表柔比星-TACE组差异无统计学意义（P > 0.05），GSPs-TACE组与表柔比星-TACE组相比，GSPs+表柔比星-TACE组肿瘤体积、肝内转移占比、腹腔内转移占比、VEGF、Bcl-2均降低（P < 0.05），肿瘤细胞凋亡率、Bax、Bcl-2/Bax、caspase-3均升高（P < 0.05）。 结论 明胶海绵微粒化疗联合肝动脉化疗栓塞抑制兔肝移植瘤瘤体生长。 Abstract:Objective To investigate the effect of gelatin sponge particle chemotherapy combined with hepatic artery chemoembolization on the growth of rabbit liver transplant tumors. Method Sixty New Zealand white rabbits were randomly divided into a model control group and gelatin sponge particles （GSPs）- Transcatheterarterial chemoembolization. The TACE group, the epirubicin-TACE group and the GSPs+ epirubicin-TACE group （n = 15）. VX2 cell liver cancer models were established in all experimental rabbits. After the operation, in the GSPS-TACE group, GSPs were perfused during TACE. In the epirubicin-TACE group, epirubicin solution （1mg/mL） was perfused. In the GSPs+ epirubicin-TACE group, a mixture of epirubicin solution and GSPs was used for embolization. Computed tomography was used to detect the tumor volume of experimental rabbits in each group. Hematoxylin-eosin staining was used to observe the pathological morphology. The apoptosis rate of tumor cells was detected by Terminal Deoxynucleotidyl Transferase-Mediated dUTPNick End Labeling （TUNEL） staining; Immunohistochemical detection of positive expression of vascular endothelial growth factor （VEGF）; Western blotting was used to detect B-cell lymphoma 2 （Bcl-2）, Bcl2-associated X protein （Bax）, and cysteine-aspartic acid Protein expression of protease-3, caspase-3. Results Compared with the model control group, the tumor volume, the proportion of intrahepatic metastasis, the proportion of intra-abdominal metastasis, VEGF, and Bcl-2 in the GSPs-TACE group were all decreased （P < 0.05）, while the apoptosis rate of tumor cells, Bax, Bcl-2/Bax, and caspase-3 were all increased （P < 0.05）. There was no statistically significant difference between the GSPS-TACE group and the epirubicin-TACE group （P > 0.05）. Compared with the epirubicin-TACE group, the tumor volume, the proportion of intrahepatic metastasis, the proportion of intra-abdominal metastasis, VEGF, and Bcl-2 in the GSPs+ epirubicin-TACE group were all decreased （P < 0.05）. The apoptosis rate of tumor cells, Bax, Bcl-2/Bax, and caspase-3 all increased （P < 0.05）. Conclusion Gelatin sponge microparticle chemotherapy combined with hepatic artery chemoembolization inhibits the growth of rabbit liver transplant tumors. -
图 1 各组实验兔肿瘤体积、肝内转移、腹腔转移的比较（$ \bar x \pm s$，n = 15）
注：A：各组瘤体体积；B：各组肝内转移例数；C：各组腹腔内转移例数
Figure 1. Comparison of tumor volume，intrahepatic metastasis and abdominal metastasis in each group of experimental rabbits
图 2 各组实验兔瘤体组织病理形态比较（×200，苏木精-伊红染色）
Figure 2. Comparison of histopathological morphology of tumor tissues in experimental rabbits of each group （×200，hematoxylin-eosin staining）
图 3 各组实验兔肿瘤细胞凋亡率比较（$ \bar x \pm s$，n = 15）
Figure 3. Comparison of apoptosis rates of tumor cells in each group of experimental rabbits
图 4 各组实验兔肿瘤细胞凋亡图（200×）
Figure 4. Apoptosis diagrams of tumor cells in each group of experimental rabbits （200×）
图 5 免疫组化检测VEGF阳性表达（200×）
Figure 5. Positive expression of VEGF detected by immunohistochemistry （200×）
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