Antibacterial Effect of Slightly Acidic Electrolyzed Water on <i>Enterococcus faecalis</i> Biofilm in Root Canal in Vitro

Qian JIANG; Yu SUN; Li-jun HUO; Ya-yan LEI; Luo-wei PEI

doi:10.12259/j.issn.2095-610X.S20210303

Volume 42 Issue 3

Apr. 2021

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Qian JIANG, Yu SUN, Li-jun HUO, Ya-yan LEI, Luo-wei PEI. Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro[J]. Journal of Kunming Medical University, 2021, 42(3): 5-9. doi: 10.12259/j.issn.2095-610X.S20210303

Citation:

Qian JIANG, Yu SUN, Li-jun HUO, Ya-yan LEI, Luo-wei PEI. Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro[J]. Journal of Kunming Medical University, 2021, 42(3): 5-9. doi: 10.12259/j.issn.2095-610X.S20210303

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Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro

doi: 10.12259/j.issn.2095-610X.S20210303

1.
Dept. of Dental Endodontics，The Affiliated Stomatology Hospital of Kunming Medical University，Kunming，Yunnan 650100
2.
Shanghai Fuqiangwang Hygiene Products Co.，Ltd.，Shanghai 200000，China

Received Date: 2020-12-14
Publish Date: 2021-04-09

Abstract

Abstract

Objective To study the antibacterial effect of slightly acidic electrolyzed water （SAEW） as root canal irrigation on Enterococus faecalis （E. faecalis） biofilm in root canal in vitro. Methods E. faecalis biofilms were cultured in the root canals of isolated teeth and washed with SAEW at concentrations of 50 mg/L and 60 mg/L for 1 min, 3 mins, and 5 mins, respectively, the 1% NaClO solutions was used as positive controls. The field emission scanning electron microscope （FE-SEM） was used to observe E. faecalis biofilm morphological changes, the confocal laser scanning microscope （CLSM） combined with Live/Dead fluorescence staining was used to observe the E. faecalis biofilms and analyze the proportion of dead bacteria in the biofilms. Results FE-SEM observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaCl Osolutions with increasing washing time, E.faecalis in the biofilm decreased, the surface of the was rough, and the cells shrank. Confocal laser scanning microscope （CLSM） combined with Live/Dead fluorescence staining observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaClO solutions with increasing washing time, the red fluorescence intensity gradually increased, and the proportion of dead bacteria gradually increased （P < 0.05）, the sterilization ratio of 1% NaClO to E. faecalis at the time point of 1 min, 3 min, and 5 min was significantly higher than 50 mg/L SAEW, 60 mg/L SAEW （P < 0.05）. Conclusion Slightly acidic electrolyzed water has a certain antibacterial effect on strains in E. faecalis biofilm, but the effect is lower than 1% NaCIO solutions.
- Slightly acidic electrolyzed water,
- Enterococus faecalis,
- Biofilm,
- Sodium hypochlorite,
- Antibacteria

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References

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