Zhuwei GAO, Xinghui CHEN, Junjing HE, Hongxian LI, Lisha MA. Effect of Matrix Radiofrequency Combined with Biofeedback Electrical Stimulation on Uterine Prolapse[J]. Journal of Kunming Medical University, 2022, 43(6): 127-131. doi: 10.12259/j.issn.2095-610X.S20220604
Citation: Qian JIANG, Yu SUN, Li-jun HUO, Ya-yan LEI, Luo-wei PEI. Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro[J]. Journal of Kunming Medical University, 2021, 42(3): 5-9. doi: 10.12259/j.issn.2095-610X.S20210303

Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro

doi: 10.12259/j.issn.2095-610X.S20210303
  • Received Date: 2020-12-14
  • Publish Date: 2021-04-09
  •   Objective  To study the antibacterial effect of slightly acidic electrolyzed water (SAEW) as root canal irrigation on Enterococus faecalisE. faecalis) biofilm in root canal in vitro.  Methods  E. faecalis biofilms were cultured in the root canals of isolated teeth and washed with SAEW at concentrations of 50 mg/L and 60 mg/L for 1 min, 3 mins, and 5 mins, respectively, the 1% NaClO solutions was used as positive controls. The field emission scanning electron microscope (FE-SEM) was used to observe E. faecalis biofilm morphological changes, the confocal laser scanning microscope (CLSM) combined with Live/Dead fluorescence staining was used to observe the E. faecalis biofilms and analyze the proportion of dead bacteria in the biofilms.  Results  FE-SEM observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaCl Osolutions with increasing washing time, E.faecalis in the biofilm decreased, the surface of the was rough, and the cells shrank. Confocal laser scanning microscope (CLSM) combined with Live/Dead fluorescence staining observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaClO solutions with increasing washing time, the red fluorescence intensity gradually increased, and the proportion of dead bacteria gradually increased (P < 0.05), the sterilization ratio of 1% NaClO to E. faecalis at the time point of 1 min, 3 min, and 5 min was significantly higher than 50 mg/L SAEW, 60 mg/L SAEW (P < 0.05).  Conclusion  Slightly acidic electrolyzed water has a certain antibacterial effect on strains in E. faecalis biofilm, but the effect is lower than 1% NaCIO solutions.
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