Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis

Na PI; Qin HE; Yan ZHONG; Xuan JIN; Zhen-dong ZHU; Xuan ZHANG

doi:10.12259/j.issn.2095-610X.S20210402

Volume 42 Issue 4

Apr. 2021

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Na PI, Qin HE, Yan ZHONG, Xuan JIN, Zhen-dong ZHU, Xuan ZHANG. Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis[J]. Journal of Kunming Medical University, 2021, 42(4): 8-12. doi: 10.12259/j.issn.2095-610X.S20210402

Citation:

Na PI, Qin HE, Yan ZHONG, Xuan JIN, Zhen-dong ZHU, Xuan ZHANG. Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis[J]. Journal of Kunming Medical University, 2021, 42(4): 8-12. doi: 10.12259/j.issn.2095-610X.S20210402

Citation:

Na PI, Qin HE, Yan ZHONG, Xuan JIN, Zhen-dong ZHU, Xuan ZHANG. Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis[J]. Journal of Kunming Medical University, 2021, 42(4): 8-12. doi: 10.12259/j.issn.2095-610X.S20210402

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Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis

doi: 10.12259/j.issn.2095-610X.S20210402

Na PI¹,
Qin HE¹,
Yan ZHONG¹,
Xuan JIN¹,
Zhen-dong ZHU^{2
,
,},
Xuan ZHANG^{1
,
,}

1.
School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Geriatrics，The 3rd People’s Hospital of Yunnan Province，The 2nd Affiliated Hospital of Dali University，Kunming Yunnan 650011，China

Received Date: 2020-12-07
Publish Date: 2021-04-01

Abstract

Abstract

Objective To study the effect of Gentiana rigescens Franch on the protein expression of NF - κ B and CTGF in lung tissue of mice with pulmonary fibrosis. Methods The model of pulmonary fibrosis in Kunming mice was established by intratracheal instillation of bleomycin （5 mg·kg^-1）. Low, medium and high doses （50, 100, 200 mg·kg^-1） of Gentiana rigescens Franch were administered intragastrically once a day. Pirfenidone was used as positive control. The mice were killed on the 28th day after modeling, and the lung tissues were taken for detection. Gross pathological changes of lung tissue were observed by naked eyes. Masson staining was used to observe the degree of pulmonary fibrosis. Western blot was used to detect the expression of NF - κ B and CTGF in lung tissue. Results Compared with the model group, the collagen deposition in lung tissue of mice was significantly reduced in different doses of Gentiana rigescens group and positive drug Pirfenidone group. Compared with the model group, the protein expression of NF - κ B and CTGF in lung tissue of mice were significantly down-regulated in different doses of Gentiana rigescens group and positive drug bifidone group （P < 0.05）. Conclusion Gentiana rigescens Franch has anti-pulmonary fibrotic effect, the anti-fibrotic mechanism is related to inhibiting lung inflammation and collagen deposition througth down-regulating the protein expression of NF - κ B and CTGF.
- Pulmonary fibrosis,
- Gentiana rigescens franch,
- Nulcear factor-κB,
- Connective tissue growth factor

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References(19)

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