Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits

Jing ZHOU; Ping XIONG; Chao-feng LIU; Li-qiong CHEN; Yong-hui ZHANG; Ke SHI; Yan NIE; Yan LIU

doi:10.12259/j.issn.2095-610X.S20210503

Volume 42 Issue 5

May 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(5): 12-17

Jing ZHOU, Ping XIONG, Chao-feng LIU, Li-qiong CHEN, Yong-hui ZHANG, Ke SHI, Yan NIE, Yan LIU. Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits[J]. Journal of Kunming Medical University, 2021, 42(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20210503

Citation:

Jing ZHOU, Ping XIONG, Chao-feng LIU, Li-qiong CHEN, Yong-hui ZHANG, Ke SHI, Yan NIE, Yan LIU. Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits[J]. Journal of Kunming Medical University, 2021, 42(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20210503

Citation:

Jing ZHOU, Ping XIONG, Chao-feng LIU, Li-qiong CHEN, Yong-hui ZHANG, Ke SHI, Yan NIE, Yan LIU. Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits[J]. Journal of Kunming Medical University, 2021, 42(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20210503

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Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits

doi: 10.12259/j.issn.2095-610X.S20210503

1.
Dept. of Stomatology，Yan’an Hospital Affiliated of Kunming Medical University，Kunming Yunnan 650100
2.
The 2nd clinic，The Affiliated Stomatology Hospital of Kunming Medical University，Kunming Yunnan 650100，China

Received Date: 2021-01-29
Available Online: 2021-06-02
Publish Date: 2021-05-20

Abstract

Abstract

Objective It is planned to build a New Zealand rabbit periodontal defect model and determine the biological safety of hPDLSCs-BMP-2-PSH membrane and its in vivo osteogenic repair effect. Methods After confirming that the BMP-2-PSH membrane was not cytotoxic, the cultured hPDLSCs cell membrane was compounded to the BMP-2-PSH membrane. Respectively, HPDLSCs/PSH composite membrane was implanted into the alveolar bone defect of mandibular left incisor in each New Zealand rabbit as the experimental group, and the controlled porous fiber membrane material was implanted into alveolar bone defect of the right central incisor as the control group （or not implanted any material was used as a control）, then the whole flap was reset and sutured. Imaging （CT） examination was performed immediately after the surgery and every 3 weeks （observed continuously for 12 weeks） to observe the healing of bone defects; meanwhile, the absorbance of PDLSCs/BMP-2 double membrane materials, the amount of new bone formation and new bone morphology of samples in each group were observed at 4, 8 and 12 weeks after the surgery. Results Different concentrations of PSH membrane and BMP-2-PSH membrane extracts were not cytotoxic to hPDLSCs cells, hPDLSCs cells could be successfully compounded to BMP-2-PSH membranes; The analysis of CBCT and histomorphology showed that hPDLSCs/PSH composite membranes can effectively promote the repair and regeneration of alveolar bone defects. Conclusion The hPDLSCs/PSH composite membrane has the good biological safety and is beneficial to repair periodontal defects of New Zealand rabbits.
- Periodontal ligament stem cells,
- BMP-2-PSH composite membrane,
- Periodontal regeneration

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References(17)

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