Changes of c-Fos Protein and Structural Plasticity in Nucleus Accumbens of Mice after Ultrasound Stimulation

Zhen-dong YANG; Yun-yan LAN; Yu ZHENG; Yu-ran FENG; Mei ZHU

doi:10.12259/j.issn.2095-610X.S20210645

Volume 42 Issue 6

Jul. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(6): 45-49

Zhen-dong YANG, Yun-yan LAN, Yu ZHENG, Yu-ran FENG, Mei ZHU. Changes of c-Fos Protein and Structural Plasticity in Nucleus Accumbens of Mice after Ultrasound Stimulation[J]. Journal of Kunming Medical University, 2021, 42(6): 45-49. doi: 10.12259/j.issn.2095-610X.S20210645

Citation:

Zhen-dong YANG, Yun-yan LAN, Yu ZHENG, Yu-ran FENG, Mei ZHU. Changes of c-Fos Protein and Structural Plasticity in Nucleus Accumbens of Mice after Ultrasound Stimulation[J]. Journal of Kunming Medical University, 2021, 42(6): 45-49. doi: 10.12259/j.issn.2095-610X.S20210645

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Changes of c-Fos Protein and Structural Plasticity in Nucleus Accumbens of Mice after Ultrasound Stimulation

doi: 10.12259/j.issn.2095-610X.S20210645

Dept. of Ultrasound，The 1 st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-04-06
Available Online: 2021-07-03
Publish Date: 2021-07-21

Abstract

Abstract

Objective To observe the neuronal activation and structural changes after the stimulation of the nucleus accumbens （NAc） in mice by detecting c-Fos protein and structural plasticity, and to preliminally explore the mechanism of neural regulation by ultrasound. Methods C57 BL/6 mice were randomly divided into ultrasonic stimulation group （n = 12） and sham stimulation group （n = 6）. In the ultrasound stimulation group, low intensity focused ultrasound was used to stimulate the NAc region of mice with fixed parameters. After 7 days of continuous stimulation, the expression of c-Fos protein in the NAC region was determined by immunohistochemistry, and the changes of neuronal plasticity were observed by electron microscopy. The sham stimulation group was also irradiated with ultrasonic stimulation probe, and no output power was obtained without turning on the machine. Results All mice successfully completed the whole process of ultrasonic stimulation. The expression ratio of c-fos in NAC neurons was significantly increased after ultrasound stimulation （P < 0.001）. Under electron microscope, it was found that the distribution of dendritic spines in the ultrasonic stimulation group was sparse, the number of dendritic spines was significantly reduced, and the morphology was mushroom-like or dwarf stump-like. Compared with the sham stimulation group, the density of dendritic spines was significantly reduced （P < 0.0001）, and the length of dendrites was significantly shortened （P < 0.0001）. Conclusion Low intensity focused ultrasound can significantly activate neurons in NAc brain region, and inhibit and regulate the structural plasticity of neurons, which proves that ultrasound stimulation has the potential to intervene and treat brain diseases.
- Ultrasonic stimulation,
- Neuromodulation,
- c-Fos,
- Structural plasticity.

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References(14)

References

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