Volume 43 Issue 4
Apr.  2022
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Yixun LI, Kun WU, Rui CHEN, Liying WANG, Zhuoma SINAN, Chunyan XIA. Evaluation of Red Blood Cell Fragment Parameter Detection of Automatic Hematology Analyzer[J]. Journal of Kunming Medical University, 2022, 43(4): 93-98. doi: 10.12259/j.issn.2095-610X.S20220415
Citation: Yixun LI, Kun WU, Rui CHEN, Liying WANG, Zhuoma SINAN, Chunyan XIA. Evaluation of Red Blood Cell Fragment Parameter Detection of Automatic Hematology Analyzer[J]. Journal of Kunming Medical University, 2022, 43(4): 93-98. doi: 10.12259/j.issn.2095-610X.S20220415

Evaluation of Red Blood Cell Fragment Parameter Detection of Automatic Hematology Analyzer

doi: 10.12259/j.issn.2095-610X.S20220415
  • Received Date: 2022-02-12
    Available Online: 2022-04-02
  • Publish Date: 2022-04-25
  •   Objective   Fragmented red blood cells (FRC) is a new detection parameter provided by the automatic blood cell analyzer, which is closely related to thrombotic microangiopaemia and other diseases. This study is aimed at evaluating the detection performance of automatic hematology analyzer for RBC fragments.   Methods  The performance of SYSMEX XN series automatic blood cell analyzer was evaluated by carrying contamination rate, precision, linearity, accuracy, sensitivity, specificity and other indicators. All data were analyzed by SPSS 19.0 software.   Results  The contamination rate of red blood cell fragments detected by SYSMEX XN series automatic hematology analyzer was 0.11%. Intra-batch precision: coefficient of variation (CV) at each experimental concentration were 8.75% (42.15×109/L), 6.38% (83.42×109/L), 4.57% (228.74×109/L), 5.31% (796.89×109/L). Linear verification: the linear regression equation was Y = 1.0001X + 9.3288, and the correlation coefficient is r = 0.9936., and the correlation coefficient is R =0.9936; Correctness verification: compared with manual microscopy, the was no statistically significant difference(P>0.05), the correlation coefficient, sensitivity and specificity were r = 0.82, 92% and 82% respectively.   Conclusion   SYSMEX XN series automatic blood cell analyzer is quick and easy to operate in detecting red blood cell fragments, with low contamination rate, fair precision and satisfactory linear range. Compared with manual microscopy, it has better correlation, higher specificity and sensitivity. Blood cell analyzer can be used as a rapid screening method for fragments of peripheral blood red blood cells, but the precision of its detection still needs to be further improved, and due to the limitations of the method, positive specimens need to be confirmed under a microscope.
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