Determination of 29 Trace Elements in 32 Cases of Liver Biopsy with Unexplained Liver Injury

Huimin LI; Gang CHEN; Tiehan LI; Xiaojie FENG; Weikun LI; Yanwei QI; Chunyun LIU; Li LIU

doi:10.12259/j.issn.2095-610X.S20220419

Volume 43 Issue 4

Apr. 2022

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > 43(4): 125-131

Huimin LI, Gang CHEN, Tiehan LI, Xiaojie FENG, Weikun LI, Yanwei QI, Chunyun LIU, Li LIU. Determination of 29 Trace Elements in 32 Cases of Liver Biopsy with Unexplained Liver Injury[J]. Journal of Kunming Medical University, 2022, 43(4): 125-131. doi: 10.12259/j.issn.2095-610X.S20220419

Citation:

Huimin LI, Gang CHEN, Tiehan LI, Xiaojie FENG, Weikun LI, Yanwei QI, Chunyun LIU, Li LIU. Determination of 29 Trace Elements in 32 Cases of Liver Biopsy with Unexplained Liver Injury[J]. Journal of Kunming Medical University, 2022, 43(4): 125-131. doi: 10.12259/j.issn.2095-610X.S20220419

Citation:

Huimin LI, Gang CHEN, Tiehan LI, Xiaojie FENG, Weikun LI, Yanwei QI, Chunyun LIU, Li LIU. Determination of 29 Trace Elements in 32 Cases of Liver Biopsy with Unexplained Liver Injury[J]. Journal of Kunming Medical University, 2022, 43(4): 125-131. doi: 10.12259/j.issn.2095-610X.S20220419

PDF( 679 KB)

Determination of 29 Trace Elements in 32 Cases of Liver Biopsy with Unexplained Liver Injury

doi: 10.12259/j.issn.2095-610X.S20220419

1.
Dept. of Hepatology，The 3rd People’s Hospital of Kunming, Kunming Yunnan 650041
2.
Dept. of Hepatobiliary and Pancreatic Surgery，The 1st People’s Hospital of Kunming，Kunming Yunnan 650034
3.
Dept. of Liver and Gallbladder Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
4.
Dept.of Pathology，The 1st People’s Hospital of Kunming, Kunming Yunnan 650034，China

Received Date: 2022-01-01
Available Online: 2022-03-15
Publish Date: 2022-04-25

Abstract

Abstract

Objective To determine trace elements in liver tissue and to explore the relationship between the occurrence of liver injury with unknown causes and trace elements in the body. Methods The liver tissue of patients with liver injury of unknown causes was obtained by liver biopsy. The content of 29 trace elements was determined by inductively coupled plasma mass spectrometry （ICP-MS） and were compared with the normal liver tissue of the control group. Results Compared with the control group, 19 elements including lithium, beryllium, aluminum, calcium, titanium, vanadium, chromium, cobalt, nickel, germanium, strontium, molybdenum, indium, tin, rhenium, platinum, mercury, lead, bismuth were significantly increased （P < 0.01）. Arsenic and selenium increased （P < 0.05）, and cadmium significantly decreased （P < 0.01）. However, there were no significant differences in magnesium, manganese, ferrum, copper, zinc, gallium, selenium and barium （P > 0.05）. Spearman correlation analysis showed ferritin was positively correlated with iron content in liver tissue. Conclusion The increase of trace elements in liver tissue of patients with liver injury of unknown cause indicated that abnormal metabolism of trace elements might be one of the causes of liver injury of unknown causes or play a certain role in the occurrence and development of liver disease. By examination of liver pathology and determination of trace elements in liver tissue, the causes of liver damage in the experimental group were clearly found. Ferritin was positively correlated with iron content in liver tissue.
- liver injury with unknown causes,
- Liver tissue biopsy,
- Determination of trace elements

FullText(HTML)

References(30)

References

[1]	杨永峰. 不明原因肝病诊断思路[J]. 实用肝脏病杂志,2018,21(1):1-3. doi: 10.3969/j.issn.1672-5069.2018.01.001
[2]	臧红,郭晓东,游绍莉,等. 不明原因肝功能异常患者的临床诊断思维[J]. 现代生物医学进展,2013(2):263-265.
[3]	游绍莉,朱冰,辛绍杰,等. 对疑难肝病临床诊断的认识与思考[J]. 传染病信息,2012,25(6):336-338. doi: 10.3969/j.issn.1007-8134.2012.06.006
[4]	汪佩文,董育玮,李郑红,等. 94例不明原因肝功能异常患者临床与肝组织病理学分析[J]. 实用肝脏病杂志,2018,21(2):225-228. doi: 10.3969/j.issn.1672-5069.2018.02.017
[5]	施先义 ,韦文业. 人体中的必需微量元素与有毒元素[J]. 科技创新导报,2010,4(4):104.
[6]	黄福林. 慢性铅中毒与肝功能损害的临床分析[J]. 中国医药指南,2018,16(7):133-134.
[7]	李小安,温彪,朱丹,等. 慢性肝病患者微量元素的研究[J]. 临床和实验医学杂志,2011,10(5):338-339.
[8]	杨瑞园,赵新颜. 肝组织病理学检查在药物性肝损伤诊治中的意义[J]. 临床肝胆病杂志,2018,34(6):1172-1175. doi: 10.3969/j.issn.1001-5256.2018.06.007
[9]	池肇春. 必需微量元素与肝病[J]. 中西医结合肝病杂志,2010,20(2):65-68.
[10]	中华医学会肝病学分会,脂肪肝和酒精性肝病学组,中国医师协会脂肪性肝病专家委员会. 非酒精性脂肪性肝病诊疗指南(2018年更新版)[J]. 临床肝胆病杂志,2018,(5):947-957.
[11]	中华医学会肝病学分会,中华医学会消化病学分会,中华医学会感染病学分会. 自身免疫性肝炎诊断和治疗共识(2015)[J]. 临床肝胆病杂志,2016,23(1):9-22. doi: 10.3969/j.issn.1001-5256.2016.01.002
[12]	中华医学会肝病学分会,消化病学分会,感染病学分会. 胆汁淤积性肝病诊断和治疗共识(2015)[J]. 临床肝胆病杂志,2015,31(12):1989-1999. doi: 10.3969/j.issn.1001-5256.2015.12.005
[13]	中华医学会肝病学分会,药物性肝病学组. 2015 年版药物性肝损伤诊治指南[J]. 中华肝脏病杂志,2015,23(11):810-820. doi: 10.3760/cma.j.issn.1007-3418.2015.11.004
[14]	王志平,周连芳. 微量元素钒在人体中的作用[J]. 集宁师专学报,2002,24(4):51-66.
[15]	段玉梅. 必需微量金属元素在人体中的作用及毒性[J]. 生物学通报,2004,39(6):25-26.
[16]	岑枝梅,刘金涛,冯铁柱. 各种肝病患者血清微量元素分析[J]. 实用肝脏病杂志,2012,15(5):434-435.
[17]	任丽燕,刘浩宇,刘锡仪. 肝脏疾病与矿物元素的关系[J]. 广东微量元素科学,2006,13(10):1-5.
[18]	邵祥稳,张庆华. 肝硬化患者血清微量元素变化及临床意义[J]. 中国临床医生杂志,2017,45(8):45-46.
[19]	赖春颜,李宗良,王向槐. 肝衰竭、肝硬化患者的血清微量元素变化[J]. 广东微量元素科学,2013,20(1):1-4.
[20]	王保宏,李义锋,曹晓丽,等. 无机盐和肝脏疾患的关系[J]. 中国伤残医学年,2013,21(11):472-475.
[21]	金明丽,舒健. 肝脏铁质浓度测量的研究进展[J]. 泸州医学院学报,2016,39(1):79-81.
[22]	刘虎生,诸洪达,欧阳荔,等. 电感耦合等离子体质谱测定人肝中48种微量元[J]. 现代科学仪器,2006,16(5):68-69. doi: 10.3969/j.issn.1003-8892.2006.05.017
[23]	诸洪达,张永保,王松君,等. 52 例中国人主要器官、组织中 37 种元素的浓度[J]. 中华放射医学与防护杂志,2004,24(3):211-216. doi: 10.3760/cma.j.issn.0254-5098.2004.03.009
[24]	闫芳,闫伟群,魏新光,等. 潍坊市100 例正常人体肝组织微量元素测定[J]. 微量元素与健康研究,2000,17(3):12.
[25]	朱远方,成志芳,朱素娟,等. 肝内胆管结石病人肝组织、血液、胆汁及胆石中微量元素测定[J]. 江苏临床医学杂志,1998,2(3):232-233.
[26]	Varga I,Szebeni A,Szoboszlai N,et al. Determination of trace elements in human liver biopsy samples by ICP-MS and TXRF:Hepatic steatosis and nickel accumulation.[J]. Anal Bioanal Chem,2005,383(3):476-482. doi: 10.1007/s00216-005-0010-0
[27]	Valenti L,Fracanzani A L,Bugianesi E,et al. HFE genotype,parenchymal iron accumulation,and liver fibrosis in patients with nonalcoholic fatty liver disease.[J]. Gastroenterology,2010,138(3):905-912. doi: 10.1053/j.gastro.2009.11.013
[28]	Yang X,Tang X P,Zhang Y H,et al. Prospective evaluation of the diagnostic accuracy of hepatic copper content,as determined using the entire core of a liver biopsy sample.[J]. Hepatology,2015,62(6):1731-1741. doi: 10.1002/hep.27932
[29]	Hendy O M,Allam M,Allam A,et al. Hepcidin levels and iron status in beta-thalassemia major patients with hepatitis C virus infection.[J]. Egypt J Immunol,2010,17(2):33-44.
[30]	陈学平,罗志刚,曹丽玲,等. 不明原因肝损害的诊断特点和肝组织病理特征115例[J]. 世界华人消化杂志,2014,22(12):1730-1733.

Relative Articles(20)

Relative Articles

[1]	Liyuan WANG, Zhenting YANG, Qingling LI, Haiwen LI, Zhirong ZHAO, Yongrui YANG, Shude LI. Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet. Journal of Kunming Medical University, 2023, 44(4): 9-15. doi: 10.12259/j.issn.2095-610X.S20230408
[2]	Gang LI, Shaohua YANG, Meng SHEN, Nan XIE, Guanbo ZHANG, Jiaxing WANG, Jinsong LI. Expression and Clinical Significance of Eukaryotic Translation Initiation Factors 5A2 and Ki67 in Intrahepatic Cholangiocarcinoma. Journal of Kunming Medical University, 2022, 43(6): 7-12. doi: 10.12259/j.issn.2095-610X.S20220630
[3]	Cai He Lun , Li Ping , Dong Yan , Chen Jun Wen , Dong Zhao Xing . . Journal of Kunming Medical University, 2020, 41(08): 94-99.
[4]	Shi Ya Xing , Zhou Jia , Wang Xiao Ying , Qian Zhong Yi , Shen Li Juan , Zhang Zong Ji . . Journal of Kunming Medical University, 2020, 41(04): 103-110.
[5]	Li Xiao Ming , Chen Xiao Bo , Li Guang Jian , Zhao Guang Qiang , Huang Yun Chao . . Journal of Kunming Medical University, 2019, 40(09): 29-31.
[6]	Zhang Xian , Li Yan Hong , Li Yan , Zhao Lin , Tian Jun . . Journal of Kunming Medical University, 2019, 40(01): 24-29.
[7]	Zhuang Lin , Hu Ming Fen , Pang Xing Mei , Bai Bao Li , Wang Lin , Yang Hong Jie , Li Yun Li , Dong Zhi Jian , Huan Wen Qing , Wang Qing Qing , Yu Wei Qin , Kuang Xiao Lin . Contrast Researches of Liver Biopsy and Ultrasound in the Diagnosis of Fatty Liver. Journal of Kunming Medical University, 2018, 39(01): 85-91.
[8]	Zhang Le , Tang Li Bin , Jiang Jian Jie , Xia Jia Wei , Lv Zheng Xuan , Li Yun Zhen , Han Liu Xin , Bai Bin . 非生物型人工肝技术治疗肝衰竭合并肝肾综合征. Journal of Kunming Medical University, 2017, 38(06): 36-39.
[9]	Li Sheng Hao . Characteristic of Liver Injury Induced by Simvastatin Combined with HRZ in SD Rats. Journal of Kunming Medical University,
[10]	He Min . . Journal of Kunming Medical University,
[11]	Yang Jing . . Journal of Kunming Medical University,
[12]	Liang Yong Xue . . Journal of Kunming Medical University,
[13]	Qing De Ke . . Journal of Kunming Medical University,
[14]	Li Ruo Nan . . Journal of Kunming Medical University,
[15]	. Application Value of Precise Hepatectomy in Liver Surgery. Journal of Kunming Medical University,
[16]	. Therapeutic Effect of Supper Selectively Hepatic Artery Embolization on the Cavernous Hemangioma of Liver. Journal of Kunming Medical University,
[17]	Wang Shi Ping . Experiences in the Treatment of Chronic Empyema with Pleural Decortication. Journal of Kunming Medical University,
[18]	Xu Jing . Study of Heterografting of Microliver Slice in Rats with Fulminant Hepatic Failure. Journal of Kunming Medical University,
[19]	. . Journal of Kunming Medical University,
[20]	Lin Cui Yun . . Journal of Kunming Medical University,