Population Genetic Analysis of 18 Autosomal STR Loci in Minjia Branch of Bai Nationality in Yunnan Province, Southwest China

Wei WEI; Shouxun ZHANG; Chongchong XU; Yanling JIANG; Yi LI; Shuo YANG; Jianli HAN; Kuan LI; Fei ZHAO; Xiaopei YANG; Neng ZHANG; Xiaoxiao LU; Shurong ZHONG

doi:10.12259/j.issn.2095-610X.S20220510

Volume 43 Issue 5

May 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(5): 33-43

Wei WEI, Shouxun ZHANG, Chongchong XU, Yanling JIANG, Yi LI, Shuo YANG, Jianli HAN, Kuan LI, Fei ZHAO, Xiaopei YANG, Neng ZHANG, Xiaoxiao LU, Shurong ZHONG. Population Genetic Analysis of 18 Autosomal STR Loci in Minjia Branch of Bai Nationality in Yunnan Province, Southwest China[J]. Journal of Kunming Medical University, 2022, 43(5): 33-43. doi: 10.12259/j.issn.2095-610X.S20220510

Citation:

Wei WEI, Shouxun ZHANG, Chongchong XU, Yanling JIANG, Yi LI, Shuo YANG, Jianli HAN, Kuan LI, Fei ZHAO, Xiaopei YANG, Neng ZHANG, Xiaoxiao LU, Shurong ZHONG. Population Genetic Analysis of 18 Autosomal STR Loci in Minjia Branch of Bai Nationality in Yunnan Province, Southwest China[J]. Journal of Kunming Medical University, 2022, 43(5): 33-43. doi: 10.12259/j.issn.2095-610X.S20220510

Citation:

Wei WEI, Shouxun ZHANG, Chongchong XU, Yanling JIANG, Yi LI, Shuo YANG, Jianli HAN, Kuan LI, Fei ZHAO, Xiaopei YANG, Neng ZHANG, Xiaoxiao LU, Shurong ZHONG. Population Genetic Analysis of 18 Autosomal STR Loci in Minjia Branch of Bai Nationality in Yunnan Province, Southwest China[J]. Journal of Kunming Medical University, 2022, 43(5): 33-43. doi: 10.12259/j.issn.2095-610X.S20220510

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Population Genetic Analysis of 18 Autosomal STR Loci in Minjia Branch of Bai Nationality in Yunnan Province, Southwest China

doi: 10.12259/j.issn.2095-610X.S20220510

Wei WEI¹,
Shouxun ZHANG¹,
Chongchong XU¹,
Yanling JIANG²,
Yi LI³,
Shuo YANG¹,
Jianli HAN³,
Kuan LI¹,
Fei ZHAO^{1, 4},
Xiaopei YANG^{1, 5},
Neng ZHANG¹,
Xiaoxiao LU^{1, 6
,
,},
Shurong ZHONG^{1, 7
,
,}

1.
School of Forensic Medicine
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500
3.
Forensic Center of Public Security of Dali Prefecture，Dali Yunnan 671000
4.
Jiangxi Shenzhou Judicial Identification Center，Nanchang Jiangxi 330000
5.
Chuxiong Medical College，ChuxiongYunnan 675005
6.
Beijing Tongda Shoucheng Institute of Forensic Science，Beijing 100089
7.
Judicial Identification Center of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-01-11
Available Online: 2022-05-19
Publish Date: 2022-05-27

Abstract

Abstract

Objective To investigate the genetic polymorphism of 18 autosomal short tandem repeats （STR）loci in 2323 healthy unrelated individuals of Minjia branch of Bai nationality in Yunnan, calculate the population genetic parameters, and establish the genetic data of Minjia branch of Bai nationality in Yunnan, so as to provide the scientific basis for the parental identification and individual identification of forensic material evidence. Methods A total of 2323 unrelated individuals from Minjia branch of Bai nationality population were collected. DNA Typer^TM 19 kit was used for direct PCR compound amplification. AB 3130XL automatic genetic analyzer was used to separate the PCR compound amplification products by capillary electrophoresis. STR typing was performed with GeneMapper ID-x 1.5 software. Forensic parameters, such as allele frequency and matching probability, were calculated by Modified Powerstats software, and the hardy-Weinberg equilibrium （HWE） test was performed. The Fst and P values were calculated using Arlequin software. Nei’s genetic distance was calculated using Phylip software. Multi-dimensional scale （MDS） analysis was performed using SPSS software. The Neighbor-Joining （NJ） phylogenetic tree was constructed by Mega software. Results A total of 230 alleles and 1073 genotypes were observed at 18 STR loci. Except that D13S317 loci was not in accord with Hardy-Weinberg equilibrium （P = 0.0008）, the allele frequencies and genotype frequencies of other loci were consistent with Hardy-Weinberg equilibrium （P > 0.05/20 = 0.0025）. The cumulative discrimination power （CDP） and cumulative probability of exclusion （CPE） of 18 STR loci were 0.999999999 and 0.99999994055, respectively. The results of Fst, Nei’s genetic distance and NJ phylogenetic tree indicated that the Minjia branch of Bai nationality was close to the Bai nationality in Nujiang and the Bai nationality in Dali, but far away from the Bulang nationality in Yunnan and Va nationality in Yunnan. Conclusion The 18 STR loci mentioned above show the high genetic polymorphism in the Minjia branch of Bai nationality and they can be used for the forensic identification and population genetics research.
- Population genetic,
- Short tandem repeat,
- Genetic polymorphism,
- Minjia branch of Bai nationality

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