Hui-min WAN, Qing ZHOU. Effect of Continuing Motivational Interviewing on the Compliance of Diabetic Cataract Patients after Discharge from Day Surgery[J]. Journal of Kunming Medical University, 2020, 41(11): 165-170. doi: 10.12259/j.issn.2095-610X.S20201114
Citation: Guili LIANG, Canmei LI, Chengfeng XING, Yunlong DONG, Juan WANG, Zi LEI, Yinglei MIAO, Danfeng LAN. Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C-/- Mice[J]. Journal of Kunming Medical University, 2022, 43(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20220712

Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C-/- Mice

doi: 10.12259/j.issn.2095-610X.S20220712
  • Received Date: 2022-04-05
    Available Online: 2022-07-01
  • Publish Date: 2022-07-14
  •     Objective   To investigate the role of guanylate cyclase C (GC-C) signaling pathway in the intestinal inflammatory injury of mice induced by sodium dextran sulfate (DSS).    Methods   The colitis models were constructed using DSS in the GC-C gene knockout (GC-C-/-) mice and wild-type (WT) mice. The mice were divided into the colitis groups which were given drinking 3% DSS solution for one week, and the control groups which were given normal diet. The expressions of GC-C mRNA and protein in colon tissues were detected by the methods of qRT-PCR and western blot. The inflammatory damage of colon tissues was observed by the HE staining and the histopathological score was performed by the light microscope. The levels of inflammatory factors (IL-8 and TNF-α) in the peripheral blood as well as intestinal mucus of mice were determined by ELISA.    Results   1. Compared with the WT mice, the disease activity index (DAI) scores of the GC-C-/- mice induced by DSS increased significantly (P < 0.05), and the shortening of colon, intestinal thickening, hyperemia and edema were more obvious; 2. The inflammatory damage of colon tissues was more serious and the histopathological score was higher in the GC-C-/-+DSS group than that of the WT+DSS group significantly (P < 0.05); 3. The levels of IL-8 and TNF-αin the peripheral blood and intestinal mucus were higher in the GC-C-/-+DSS group than that of the WT+DSS group significantly (P < 0.05, respectively).    Conclusions   The intestinal inflammatory injury is more serious in the GC-C-/- mice induced by chemical substances. GC-C signaling pathway may be involved in the occurrence and development of ulcerative colitis (UC).
  • [1]
    Shah S C,Itzkowitz S H. Colorectal cancer in inflammatory bowel disease:mechanisms and management[J]. Gastroenterology,2022,162(3):715-730. doi: 10.1053/j.gastro.2021.10.035
    [2]
    Ng S C,Shi H Y,Hamidi N,et al. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century:a systematic review of population-based studies[J]. Lancet,2018,390(10114):2769-2778.
    [3]
    Benchimol E I,Bernstein C N,Bitton A,et al. Trends in epidemiology of pediatric inflammatory bowel disease in Canada:distributed network analysis of multiple population-based provincial health administrative databases[J]. Am J Gastroenterol,2017,112(7):1120-1134. doi: 10.1038/ajg.2017.97
    [4]
    Uranga J A,Castro M,Abalo R. Guanylate cyclase C:A current hot target,from physiology to pathology[J]. Curr Med Chem,2018,25(16):1879-1908.
    [5]
    Rappaport J A,Waldman S A. The guanylate cyclase C-cGMP signaling axis opposes intestinal epithelial injury and neoplasia[J]. Front Oncol,2018,8:299. doi: 10.3389/fonc.2018.00299
    [6]
    Xing C,Zhang T,Liu X,et al. Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway[J]. Acta Histochem,2022,124(1):151811. doi: 10.1016/j.acthis.2021.151811
    [7]
    Lan D,Wen Y,Dong X,et al. The endogenous ligand for guanylate cyclase-C activation reliefs intestinal inflammation in the DSS colitis model[J]. Acta Biochim Pol,2020,67(3):333-340.
    [8]
    苗政,钱家鸣,李景南,等. 两种溃疡性结肠炎小鼠模型的构建及其炎症反应机制比较[J]. 协和医学杂志,2010,1(2):145-149.
    [9]
    Baumgart D C,Le Berre C. Newer Biologic and Small-Molecule Therapies for Inflammatory Bowel Disease[J]. N Engl J Med,2020,385(14):1302-1315.
    [10]
    Su H J,Chiu Y T,Chiu C T,et al. Inflammatory bowel disease and its treatment in 2018:Global and Taiwan residents status updates[J]. J Formos Med Assoc,2019,118(7):1083-1092.
    [11]
    Sasson A N,Ananthakrishnan A N,Raman M. Diet in Treatment of Inflammatory Bowel Diseases[J]. Clin Gastroenterol Hepatol,2021,19(3):425-435.
    [12]
    Camilleri M. Guanylate cyclase C agonists:emerging gastrointestinal therapies and actions[J]. Gastroenterology,2015,148(3):483-487. doi: 10.1053/j.gastro.2015.01.003
    [13]
    Lan D,Niu J,Miao J,et al. Expression of guanylate cyclase-C,guanylin,and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index[J]. Sci Rep,2016,6:25034. doi: 10.1038/srep25034
    [14]
    Wang Y J,Lan D F,Niu J K,et al. Guanylate cyclase-C signaling pathway regulates intestinal inflammatory injury and epithelial barrier function in Caco-2 cells[J]. Int J Clin Exp Pathol,2016,9(9):8858-8868.
    [15]
    Harmel-Laws E,Mann E A,Cohen M B,et al. Guanylate cyclase C deficiency causes severe inflammation in a murine model of spontaneous colitis[J]. PLoS One,2013,8(11):e79180. doi: 10.1371/journal.pone.0079180
    [16]
    Fiskerstrand T,Arshad N,Haukanes B I,et al. Familial diarrhea syndrome caused by an activating GUCY2C mutation[J]. N Engl J Med,2012,366(17):1586-1595. doi: 10.1056/NEJMoa1110132
    [17]
    Mishra V,Bose A,Kiran S,et al. Gut-associated cGMP mediates colitis and dysbiosis in a mouse model of an activating mutation in GUCY2C[J]. J Exp Med,2021,218(11):e20210479c. doi: 10.1084/jem.20210479
    [18]
    Steinbrecher K A,Harmel-Laws E,Garin-Laflam M P,et al. Murine guanylate cyclase C regulates colonic injury and inflammation[J]. J Immunol,2011,186(12):7205-7214. doi: 10.4049/jimmunol.1002469
    [19]
    Lin J E,Snook A E,Li P,et al. GUCY2C opposes systemic genotoxic tumorigenesis by regulating AKT-dependent intestinal barrier integrity[J]. PLoS One,2012,7(2):e31686. doi: 10.1371/journal.pone.0031686
    [20]
    Han X,Mann E,Gilbert S,et al. Loss of guanylyl cyclase C (GCC) signaling leads to dysfunctional intestinal barrier[J]. PLoS One,2011,6(1):e16139. doi: 10.1371/journal.pone.0016139
    [21]
    Li P, Lin J E, Chervoneva I, et al. Homeostatic control of the crypt-villus axis by the bacterial enterotoxin receptor guanylyl cyclase C restricts the proliferating compartment in intestine. Am J Pathol, 2007, 171(6): 1847-1858.
  • Relative Articles

    [1] Hanyu ZHANG, Juan LUO, Mingzhi DONG, Qiyin CHEN, Fengrui ZHANG, Rui GUO, Junying TONG, Yinglei MIAO. Retrospective Cohort Study of Infliximab and Vederizumab in the Treatment of Moderate-to-severe Ulcerative Colitis. Journal of Kunming Medical University, 2025, 46(4): 1-9.
    [2] Bo LI, Yang SUN, Yinglei MIAO. Intestinal Microecological Changes and Therapeutics in Inflammatory Bowel Disease. Journal of Kunming Medical University, 2024, 45(4): 1-8.  doi: 10.12259/j.issn.2095-610X.S20240401
    [3] Hanyu ZHANG, Juan LUO, Mingzhi DONG, Qiyin CHEN, Fengrui ZHANG, Rui GUO, Junying TONG, Yinglei MIAO. Retrospective Cohort Study of Infliximab and Vederizumab in the Treatment of Moderate-to-severe Ulcerative Colitis. Journal of Kunming Medical University, 2024, 45(10): 36-44.  doi: 10.12259/j.issn.2095-610X.S20241006
    [4] Meizi RAO, Xinyi JIANG, Jiarong MIAO. Analysis of Risk Factors for Co-Infection of Ulcerative Colitis with Epstein-barr Virus and Cytomegalovirus. Journal of Kunming Medical University, 2024, 45(11): 87-94.  doi: 10.12259/j.issn.2095-610X.S20241113
    [5] Chunling LI, Linghua LI, Xu HAN, Jie YANG, Heng ZHANG. Clinical Observation of Treating Ulcerative Colitis with Acupuncture at Front Mu Points Combined with Oral Mesalazine. Journal of Kunming Medical University, 2024, 45(3): 72-78.  doi: 10.12259/j.issn.2095-610X.S20240311
    [6] Dan LI, Xulian WAN, Lvyu LI, Yu YUN, Guangyun LUO, Weibing LIU, Gongfu LIN, Ning LI, Yongkun LI, Weigang DUAN. Intestinal Flora in Uricase-Deficient Rats. Journal of Kunming Medical University, 2023, 44(2): 27-32.  doi: 10.12259/j.issn.2095-610X.S20230205
    [7] Jing WANG, Hongying MI, Yi ZHANG, Li LI, Jianhua YU, Liqiao LIU, Qingyu LIU, Liwei WANG. Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats. Journal of Kunming Medical University, 2023, 44(1): 25-30.  doi: 10.12259/j.issn.2095-610X.S20230124
    [8] Luqiong LIU, Tong CHEN, Yongjin ZHANG, Zhenrong XIE, Chenglu HE, Yongkun HUANG. Butyric Acid Protects Intestinal Epithelial Barrier from Injury Induced by TNFα. Journal of Kunming Medical University, 2023, 44(10): 10-17.  doi: 10.12259/j.issn.2095-610X.S20231021
    [9] Xiaolin LIU, Xiaocui CHEN, Yang SUN, Minli LI, Yinglei MIAO. Clinical Efficacy and Safety Evaluation of Fecal Bacteria Transplantation by Different Ways on Ulcerative Colitis. Journal of Kunming Medical University, 2022, 43(6): 46-51.  doi: 10.12259/j.issn.2095-610X.S20220612
    [10] Xing LIU, Hai-wen LI, Hong-li HUANG, Lin-jun SHEN, Le ZHANG, Xiang-fang LIU, Wen-lin ZHANG, Lu WANG. Effects of Probiotics Enhanced Enteral Nutrition on Gastrointestinal Function and Cytokines in Patients with Severe Acute Pancreatitis. Journal of Kunming Medical University, 2021, 42(11): 81-86.  doi: 10.12259/j.issn.2095-610X.S20211116
    [11] Jing WANG, Lian-fang TANG, Mei-qun GU, Xiao-yan XU, Jian-hua YU, Shan HE, Zi-wei LI, Kai BI, Li-qiao LIU, Qiong ZHAO, Hong-ying MI. Risk Factors and Early Clinical Characteristics of Neonatal Necrotizing Enterocolitis. Journal of Kunming Medical University, 2021, 42(11): 99-104.  doi: 10.12259/j.issn.2095-610X.S20211118
    [12] Zhang Nan , Hu Xin Yu , Dong Xian Xiang , Duan Wei Gang , Yun Yu . . Journal of Kunming Medical University, 2019, 40(06): 129-134.
    [13] Wang Rui Tao , Jia Qing An , Mou Yi Ping , Geng Zhi Min , Liu Chang . The Clinical Evaluation of Danning Tablets on Regulating Gut Flora Displacement to Eliminate Biliary Inflammation and Prevent Gallstone Formation. Journal of Kunming Medical University, 2018, 39(01): 109-112.
    [14] Tian Yun Fen , Li Li , Mi Hong Ying , Huang Fu Chun Rong , He Shan , Xu Xiao Yan , Cao Yong Jiu . The Clinical Analysis of Necrotizing Enterocolitis. Journal of Kunming Medical University, 2018, 39(02): 60-64.
    [15] Zhang An Xing , Wu Jing , Sun Yang , Miao Ying Lei . . Journal of Kunming Medical University, 2018, 39(09): 128-132.
    [16] Zhou Xiao Nan , Song Xue Lan , Yang Yuan , Ma Chen Jing , He Fang Yan . Advances and Applications in Gene Silencing. Journal of Kunming Medical University, 2018, 39(01): 131-135.
    [17] Chen Xiao Hong . Influence of Continuous Nursing Intervention on Psychological Quality and Medication Compliance in Ulcerative Colitis Patients after Discharge. Journal of Kunming Medical University,
    [18] Meng Jun . . Journal of Kunming Medical University,
    [19] Li Hong Li . . Journal of Kunming Medical University,
    [20] Ai Jia . . Journal of Kunming Medical University,
  • Cited by

    Periodical cited type(15)

    1. 殷丽. 多形式集体宣教在白内障日间病房手术患者中的应用及对术后健康知识掌握情况的影响. 基层医学论坛. 2024(15): 102-104+144 .
    2. 谢驰,陈子盎,章尧,娄炜,金海鹰. 老年性白内障患者血糖与术后视力恢复的关联. 安徽理工大学学报(自然科学版). 2024(02): 94-100 .
    3. 付琴,祝青燕,邹霞,黄勇翔,刘悠. 结构化护理干预对老年白内障手术患者自我护理依从性的影响分析. 基层医学论坛. 2024(17): 70-73 .
    4. 刘媛,于莹,李景. 延续护理减少老年白内障手术患者术后并发症的效果评价. 中外医疗. 2024(19): 134-137 .
    5. 张杨,吕长芬. 延续性护理对2型糖尿病合并老年白内障患者术后的效果评价. 糖尿病新世界. 2024(12): 21-23 .
    6. 孙东洋. PDCA循环管理联合延续护理在老年白内障患者中的应用效果. 医药前沿. 2024(27): 95-97 .
    7. 徐燕,黄娟,马剑晴. 优质护理结合电话回访在老年白内障日间手术患者中的应用效果. 中西医结合护理(中英文). 2023(02): 21-24 .
    8. 朱文娟,赵久华,臧谋红,胡俊,杨丽. 医养结合视角下养老机构中延续护理对糖尿病患者的效果评价. 南通大学学报(医学版). 2023(02): 140-143 .
    9. 王瑞,张蓓,孙捷,和心依. 专科护士主导制定的围术期点药单对眼科日间手术患者用药依从性及眼科感染发生率的影响. 海南医学. 2023(12): 1793-1796 .
    10. 应莉,黄小建. 基于ADOPT问题解决模式的护理干预在老年性白内障超声乳化术患者中的应用效果. 当代护士(上旬刊). 2023(05): 128-132 .
    11. 袁雪,刘嫚. 流程化护理干预在眼科手术患者中的应用效果及对SAS、SDS评分的影响. 临床医学研究与实践. 2023(25): 162-165 .
    12. 龙皓,汤宁,应岚. 基于信息-动机-行为技巧模型构建健康教育在中青年慢性精神分裂症患者护理工作中的应用. 心理月刊. 2023(14): 71-74 .
    13. 刘民,陈玉春. 基于ADOPT问题解决模式护理干预对玻璃体切割术患者负性情绪、依从性及并发症的影响. 国际护理学杂志. 2023(21): 3903-3907 .
    14. 卢仪真. 健康教育联合CICARE沟通模式在眼科日间病房中的应用效果. 医学理论与实践. 2022(03): 505-506 .
    15. 张倩. 早期护理干预对缓解老年白内障手术患者术前焦虑的效果分析. 中国医药指南. 2021(30): 118-119 .

    Other cited types(1)

  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(2)

    Article Metrics

    Article views (3205) PDF downloads(196) Cited by(16)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return