Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats

Wei YANG; Hongyan CHEN; Wendong CHEN; Yanqiong WANG; Xiangfeng BAI

doi:10.12259/j.issn.2095-610X.S20220724

Volume 43 Issue 7

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(7): 18-24

Wei YANG, Hongyan CHEN, Wendong CHEN, Yanqiong WANG, Xiangfeng BAI. Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats[J]. Journal of Kunming Medical University, 2022, 43(7): 18-24. doi: 10.12259/j.issn.2095-610X.S20220724

Citation:

Wei YANG, Hongyan CHEN, Wendong CHEN, Yanqiong WANG, Xiangfeng BAI. Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats[J]. Journal of Kunming Medical University, 2022, 43(7): 18-24. doi: 10.12259/j.issn.2095-610X.S20220724

Citation:

Wei YANG, Hongyan CHEN, Wendong CHEN, Yanqiong WANG, Xiangfeng BAI. Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats[J]. Journal of Kunming Medical University, 2022, 43(7): 18-24. doi: 10.12259/j.issn.2095-610X.S20220724

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Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats

doi: 10.12259/j.issn.2095-610X.S20220724

1.
Dept. of Anesthesiology
2.
Dept. of Ultrasound
3.
Dept. of Cardiovascular Surgery，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-04-16
Available Online: 2022-06-25
Publish Date: 2022-07-14

Abstract

Abstract

Objective To investigate the effect and mechanism of miR-208a on myocardial injury induced by myocardial ischemia reperfusion by regulating the expression of QKI5 in rats. Methods Thirty-two male SD rats were randomly divided into sham group, I/R group, I/R+anta-miR-NC group and I/R+anta-miR-208a group. The rat model of myocardial I/R was established, and the LVEF, LVFS and SV were detected in each group. The levels of CK-MB, cTnI, IL-1β, IL-6, TNF-αwere measured by ELISA. HE staining was used to observe the pathological changes of myocardial tissue in each group, and TUNEL staining was used to detect myocardial cell apoptosis.RT-PCR was used to detect the expression of miR-208a and QKI5 mRNA in myocardial tissue, and Western blot was used to detect the realative expression levels of QKI5 protein and Caspase-3 in myocardial tissue. Results Compared with the Sham group, the levels of LVEF, LVFS, SV in the I/R group were decreased, while the apoptosis rate of cardiomyocytes was increased, and the levels of CK-MB, cTnI, IL-1β, IL-6, TNF-α in serum and the expressions of miR-208a and Caspase-3 in the myocardial tissue were increased, and the expressions of QKI5 were decreased （P < 0.05）. Compared with the I/R+anta-miR-NC group, the levels of LVEF, LVFS, SV in the I/R+anta-miR-208a group were increased, while the apoptosis rate of cardiomyocytes was decreased, and the levels of CK-MB, cTnI, IL-1β, IL-6, TNF-α in serum and the expressions of miR-208a and Caspase-3 in the myocardial tissue were decreased, and the expressions of QKI5 were increased （P < 0.05）. Correlation analysis found that the expression of miR-208a and QKI5 had negative correlation in the I/R rats. Conclusion Inhibition of miR-208a may alleviate the inflammatory response to exert myocardial protective function in myocardial ischemia-reperfusion injury by regulating the expression of QKI5.
- Myocardial ischemia-reperfusion injury,
- miR-208a,
- QKI5 protein,
- Correlation

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References(14)

References

[1]	Mokhtari-Zaer A,Marefati N,Atkin S L,et al. The protective role of curcumin in myocardial ischemia-reperfusion injury[J]. Journal of Cellular Physiology,2018,234(1):214-222.
[2]	Russo I,Penna C,Musso T,et al. Platelets,diabetes and myocardial ischemia/reperfusion injury[J]. Cardiovascular Diabetology,2017,16(1):71. doi: 10.1186/s12933-017-0550-6
[3]	常国楫,顾金松,孙林. MicroRNA在心肌再灌注损伤应用的研究进展[J]. 昆明医科大学学报,2017,38(5):138-143. doi: 10.3969/j.issn.1003-4706.2017.05.031
[4]	Tony H,Yu K,Qiutang Z. MicroRNA-208a silencing attenuates doxorubicin induced myocyte apoptosis and cardiac dysfunction[J]. Oxidative Medicine and Cellular Longevity,2015,6(2015):597032.
[5]	Guo W,Shi X,Liu A,et al. RNA binding protein QKI inhibits the ischemia/reperfusion-induced apoptosis in neonatal cardiomyocytes[J]. Cell Physiol Biochem,2011,28(4):593-602. doi: 10.1159/000335755
[6]	Kunecki M,Płazak W,Podolec P,et al. Effects of endogenous cardioprotective mechanisms on ischemia-reperfusion injury[J]. Postepy Hig Med Dosw (Online),2017,71(10):20-31. doi: 10.5604/01.3001.0010.3786
[7]	陈耽,李骊华. miR-208a在大鼠心肌缺血再灌注损伤及缺血后处理中的表达及其作用[J]. 重庆医科大学学报,2015,4(40):501-505. doi: 10.13406/j.cnki.cyxb.000494
[8]	Kura B,Szeiffova Bacova B,Kalocayova B,et al. Oxidative Stress-Responsive MicroRNAs in Heart Injury[J]. Int J Mol Sci.,2020,21(1):358. doi: 10.3390/ijms21010358
[9]	Wang F,Yuan Y,Yang P,et al. Extracellular vesicles-mediated transfer of miR-208a/b exaggerate hypoxia/reoxygenation injury in cardiomyocytes by reducing QKI expression[J]. Mol Cell Biochem,2017,431(1-2):187-195. doi: 10.1007/s11010-017-2990-4
[10]	Yan F,Liu R,Zhuang X,et al. Salidroside attenuates doxorubicin-induced cardiac dysfunction partially through activation of QKI/FoxO1 pathway[J]. J Cardiovasc Transl Res,2021,14(2):355-364. doi: 10.1007/s12265-020-10056-x
[11]	Guo W,Jiang T,Lian C,et al. QKI deficiency promotes FoxO1 mediated nitrosative stress and endoplasmic reticulum stress contributing to increased vulnerability to ischemic injury in diabetic heart[J]. J Mol Cell Cardiol,2014,75(10):131-140.
[12]	Al-Salam S,Hashmi S. Myocardial ischemia reperfusion injury:Apoptotic,inflammatory and oxidative stress role of galectin-3[J]. Cell Physiol Biochem,2018,50(3):1123-1139. doi: 10.1159/000494539
[13]	Taki J,Wakabayashi H,Inaki A,et al. 14C-Methionine uptake as a potential marker of inflammatory processes after myocardial ischemia and reperfusion[J]. J Nucl Med,2013,54(3):431-436. doi: 10.2967/jnumed.112.112060
[14]	Li Y,Li Z,Liu J,et al. miR-190-5p alleviates myocardial ischemia-reperfusion injury by targeting PHLPP1[J]. Disease Markers,2021,2021:8709298.

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