YLR358C Regulates Cell Wall Integrity of <i>Saccharomyces cerevisiae</i>

Yu ZHANG; Mengyan LI; Hanying WANG; Yanfang GUO; Jiaqing MA; Weiqun WANG

doi:10.12259/j.issn.2095-610X.S20220730

Volume 43 Issue 7

Jul. 2022

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Yu ZHANG, Mengyan LI, Hanying WANG, Yanfang GUO, Jiaqing MA, Weiqun WANG. YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae[J]. Journal of Kunming Medical University, 2022, 43(7): 33-37. doi: 10.12259/j.issn.2095-610X.S20220730

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Yu ZHANG, Mengyan LI, Hanying WANG, Yanfang GUO, Jiaqing MA, Weiqun WANG. YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae[J]. Journal of Kunming Medical University, 2022, 43(7): 33-37. doi: 10.12259/j.issn.2095-610X.S20220730

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YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae

doi: 10.12259/j.issn.2095-610X.S20220730

1.
Faculty of Basic Medical Science
2.
Science and Technology Achievement Incubation Center，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-04-11
Available Online: 2022-06-16
Publish Date: 2022-07-14

Abstract

Abstract

Objective To observe the effect of Saccharomyces cerevisiae （S. cerevisiae） YLR358C deletion on cell wall integrity. Methods The growth of YLR358CΔ yeast was observed by spot assay; the effect of YLR358C on yeast cell wall was analyzed by Calcofluor White （CFW） staining and transmission electron microscopy （TEM）; the signal pathway regulated by YLR358C was detected by transcriptome analysis and qRT-PCR. Results The growth of S. cerevisiae knockout with YLR358C was significantly inhibited on medium containing CFW, congored （CR） and sodium dodecyl sulfate （SDS） at different concentrations. The cell wall chitin was down-regulated by CFW staining and the cell wall thickness was reduced by TEM. Transcriptome sequencing and analysis indicated that YLR358C gene may be involved in the regulation of CWI signaling pathway. qRT-PCR showed that WSC3, SWI4 and HSP12 were differentially expressed after YLR358C knockout （P < 0.05）. Conclusion YLR358C may regulate the integrity of yeast cell wall, and has certain application potential in fermentation.
- Saccharomyces cerevisiae,
- Cell wall integrity,
- YLR358C,
- Transcriptome analysis

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