Identification of Papaveraceae Based on Chloroplast DNA matK+rbcL+ trnH-psbA

Yujie FAN; Lei HUANG; Shida SU; Shaoming SU

doi:10.12259/j.issn.2095-610X.S20230104

Volume 44 Issue 1

Jan. 2023

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Yujie FAN, Lei HUANG, Shida SU, Shaoming SU. Identification of Papaveraceae Based on Chloroplast DNA matK+rbcL+ trnH-psbA[J]. Journal of Kunming Medical University, 2023, 44(1): 12-18. doi: 10.12259/j.issn.2095-610X.S20230104

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Yujie FAN, Lei HUANG, Shida SU, Shaoming SU. Identification of Papaveraceae Based on Chloroplast DNA matK+rbcL+ trnH-psbA[J]. Journal of Kunming Medical University, 2023, 44(1): 12-18. doi: 10.12259/j.issn.2095-610X.S20230104

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Identification of Papaveraceae Based on Chloroplast DNA matK+rbcL+ trnH-psbA

doi: 10.12259/j.issn.2095-610X.S20230104

1.
Yunnan Key Laboratory of Criminal Science and Technology，Kunming Yunnan 650221
2.
Institute of Criminal Science and Technology，Kunming Public Security Bureau，Kunming Yunnan 650506，China

Received Date: 2022-10-24
Available Online: 2022-12-29
Publish Date: 2023-01-18

Abstract

Abstract

Objective To explore the identification method of Papaver with the use of DNA barcoding technology and find reliable DNA barcoding sequence for intraspecific and interspecific identification of Papaver. Methods Five different species of Papaver rhoeas and Eschscholtzia californica Cham were used as intraspecific and interspecific plants for the identification of Papaver. The extracted DNA was amplified using primers matK, rbcL, trnH-psbA from chloroplast genomes and ITS2, ITS4, ITS5 from ribosomal genomes, and then bidirectional sequencing and splicing were performed using MEGA X. Sequences were analyzed and aligned, intraspecific and interspecific genetic distances were calculated, and N-J phylogenetic trees were constructed using the software. Results The sequencing results, which were entered into Genbank for comparative analysis, showed that a single DNA barcode sequence was not effective in distinguishing between Papaver somniferum and Papaver rhoeas. At the same time, a phylogenetic tree was successfully constructed using matK+rbcL+trnH-psbA combined barcode through Genbank download data, and the sequencing results of Papaver samples in this study were verified by this method. The combination of matK+rbcL+trnH-psbA barcode successfully constructed the N-J tree, which could effectively distinguish between Papaver somniferum and Papaver rhoeas in this study. Conclusion matK+rbcL+ trnH-psbA can be used as a combined DNA barcode for the identification of Papaver.
- DNA barcoding,
- matK+rbcL+trnH-psbA,
- Molecular identification,
- Papaveraceae

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References(24)

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