Effects of <i>Periplaneta americana</i> Extract on Autophagy and Invasive Metastasis of Subcutaneous Transplanted Hepatocellular Carcinoma in Nude Mice

Xuesha ZHANG; Jie ZHOU; Cailin LI; Dingyu WU; Fang PENG

doi:10.12259/j.issn.2095-610X.S20231018

Volume 44 Issue 10

Oct. 2023

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Xuesha ZHANG, Jie ZHOU, Cailin LI, Dingyu WU, Fang PENG. Effects of Periplaneta americana Extract on Autophagy and Invasive Metastasis of Subcutaneous Transplanted Hepatocellular Carcinoma in Nude Mice[J]. Journal of Kunming Medical University, 2023, 44(10): 39-46. doi: 10.12259/j.issn.2095-610X.S20231018

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Xuesha ZHANG, Jie ZHOU, Cailin LI, Dingyu WU, Fang PENG. Effects of Periplaneta americana Extract on Autophagy and Invasive Metastasis of Subcutaneous Transplanted Hepatocellular Carcinoma in Nude Mice[J]. Journal of Kunming Medical University, 2023, 44(10): 39-46. doi: 10.12259/j.issn.2095-610X.S20231018

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Effects of Periplaneta americana Extract on Autophagy and Invasive Metastasis of Subcutaneous Transplanted Hepatocellular Carcinoma in Nude Mice

doi: 10.12259/j.issn.2095-610X.S20231018

Xuesha ZHANG^{1, 2},
Jie ZHOU^{1, 2},
Cailin LI^{1, 2},
Dingyu WU^{1, 2},
Fang PENG^{1, 2
,
,}

1.
School of Pharmacy，Dali University，Yunnan Dali 671000
2.
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D，Yunnan Dali 671000，China

Received Date: 2023-05-11
Available Online: 2023-09-12
Publish Date: 2023-10-25

Abstract

Abstract

Objective To investigate the effects and preliminary mechanisms of the extract CII-3 and defatted ointment of Periplaneta americana on autophagy, invasion and metastasis of drug-resistant hepatocellular carcinoma cell line BEL-7402/5-FU. Methods The transplantation tumors of liver cancer-sensitive cells BEL-7402 and liver cancer-resistant cells BEL-7402/5-FU were treated with Sorafenib, CII-3, and defatted ointment drugs. Then, the serum biochemical indicators of the nude mice, changes in tumor size, and changes in organ indices such as heart, liver, kidney, and spleen were observed after drug administration. The histopathological changes in the liver and tumor were also observed, along with changes in autophagy-related factors and invasion and metastasis-related factors in tumor tissue cells. Results CII-3 and the deffated cream reduced the body weight of nude mice and inhibited tumor growth, significantly reducing tumor weight （P < 0.01）. CII-3 increased the spleen index and heart index （P < 0.05）; compared to the drug-resistant group, the CII-3 group showed an upward trend in serum CR （P < 0.05）. Sorafenib and CII-3 treatment induced tumor tissue necrosis, with better effects than the deffatede ointment group; compared to the drug-resistant group, both the CII-3 and deffatede ointment groups significantly inhibited autophagy and invasion-related factors, except for AKT （P < 0.05）. Conclusion Periplaneta americana extract CII-3 and deffatede ointment can inhibit the autophagy, invasion and metastasis of drug-resistant hepatocellular carcinoma cells, and also have a good anti-tumor effect. Further observation is needed for its liver and kidney damage on nude mice with hepatocellular carcinoma transplanted tumors.
- Periplaneta americana extract,
- Hepatocellular carcinoma,
- Subcutaneously transplanted tumor,
- Autophagy,
- Invasion and metastasis

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References

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