MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG

Bangqing LIU; Jianfeng LI; Xiaohui LIU; Jinnan ZHANG; Jinping LIANG

doi:10.12259/j.issn.2095-610X.S20231023

Volume 44 Issue 10

Oct. 2023

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Bangqing LIU, Jianfeng LI, Xiaohui LIU, Jinnan ZHANG, Jinping LIANG. MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG[J]. Journal of Kunming Medical University, 2023, 44(10): 83-91. doi: 10.12259/j.issn.2095-610X.S20231023

Citation:

Bangqing LIU, Jianfeng LI, Xiaohui LIU, Jinnan ZHANG, Jinping LIANG. MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG[J]. Journal of Kunming Medical University, 2023, 44(10): 83-91. doi: 10.12259/j.issn.2095-610X.S20231023

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MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG

doi: 10.12259/j.issn.2095-610X.S20231023

1.
The 2nd Dept. of Thoracic Surgery
2.
Dept. of Head and Neck Surgery，Tangshan People’s Hospital，Tangshan Hebei 063000，China

Received Date: 2023-06-26
Publish Date: 2023-10-25

Abstract

Abstract

Objective To explore the mechanisms of miR-196b affecting the progression of lung adenocarcinoma （LUAD）. Methods The expression level of miR-196b in LUAD tissues was analyzed through TCGA database. TargetScan was used to predict its downstream target genes, and dual-luciferase assay was performed to validate the predictions. qRT-PCR was used to detect the expression of miR-196b and ETS-related gene （ERG） in LUAD cell lines. MTT, scratch healing, and Transwell assays were used to respectively assess the changes in proliferation, migration, and invasion abilities of LUAD cells after different treatments. Results miR-196b was highly expressed in LUAD （P = 3.50e-17） and negatively regulated ERG. Overexpression of miR-196b promoted proliferation, migration, and invasion of LUAD cells （P < 0.05）. Rescue experiments confirmed that miR-196b/ERG regulatory axis can affect the proliferation, migration, and invasion of LUAD cells （ P < 0.05）. Conclusion The molecular mechanism of miR-196b targeting ERG in promoting the progression of LUAD has potential significance for the development of new clinical treatment methods for LUAD.
- miR-196b,
- ERG,
- Lung adenocarcinoma,
- Proliferation,
- Migration

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References(36)

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