Metformin Inhibits high Glucose-induced Vascular Smooth Muscle Cells Proliferation by Promoting Autophagy via AMPK/PPAR-γ

Ziwei ZHANG; Jialin ZHENG; Xiaoyu XU; Hong WANG

doi:10.12259/j.issn.2095-610X.S20231026

Volume 44 Issue 10

Oct. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(10): 60-66

Ziwei ZHANG, Jialin ZHENG, Xiaoyu XU, Hong WANG. Metformin Inhibits high Glucose-induced Vascular Smooth Muscle Cells Proliferation by Promoting Autophagy via AMPK/PPAR-γ[J]. Journal of Kunming Medical University, 2023, 44(10): 60-66. doi: 10.12259/j.issn.2095-610X.S20231026

Citation:

Ziwei ZHANG, Jialin ZHENG, Xiaoyu XU, Hong WANG. Metformin Inhibits high Glucose-induced Vascular Smooth Muscle Cells Proliferation by Promoting Autophagy via AMPK/PPAR-γ[J]. Journal of Kunming Medical University, 2023, 44(10): 60-66. doi: 10.12259/j.issn.2095-610X.S20231026

Citation:

Ziwei ZHANG, Jialin ZHENG, Xiaoyu XU, Hong WANG. Metformin Inhibits high Glucose-induced Vascular Smooth Muscle Cells Proliferation by Promoting Autophagy via AMPK/PPAR-γ[J]. Journal of Kunming Medical University, 2023, 44(10): 60-66. doi: 10.12259/j.issn.2095-610X.S20231026

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Metformin Inhibits high Glucose-induced Vascular Smooth Muscle Cells Proliferation by Promoting Autophagy via AMPK/PPAR-γ

doi: 10.12259/j.issn.2095-610X.S20231026

Ziwei ZHANG¹,
Jialin ZHENG¹,
Xiaoyu XU^{1, 2},
Hong WANG^{1
,
,}

1.
Center of Coronary Heart Disease，Fuwai Yunnan Cardiovascular Hospital， Kunming Yunnan 650032，China
2.
Graduate School of Kunming Medical University， Kunming Yunnan 650500，China

Received Date: 2023-08-23
Available Online: 2023-09-12
Publish Date: 2023-10-25

Abstract

Abstract

Objective To investigate the effect and regulatory mechanism of metformin on autophagy and cell proliferation of vascular smooth muscle cells （VSMCs） cultured in high glucose in mice aortic. Methods Western blot was used to detect the changes in microtubule-associated protein light chain 3-II （LC3-II） and Beclin-1 protein levels in different treatment groups. Transmission electron microscope was used to observe the the level of autophagosomes in VSMCs of each treatment group. Western blot was used to detect the expression levels of p-AMPK/AMPK and PPAR-γ proteins in different treatment groups. The EDU method was used to detect the proliferation of VSMCs in different treatment groups. Results Metformin and rapamycin reversed the inhibitory effect of high glucose on VSMC autophagy （P < 0.001）. Compound C and GW9662 enhanced the inhibitory effect of high glucose on VSMC autophagy （P < 0.05）. Metformin reversed the negative regulation of high glucose on the AMPK/PPAR-γ pathway （P < 0.01）. Metformin and rapamycin inhibited high glucose-induced VSMC proliferation （P < 0.001）, and Compound C and GW9662 reversed the inhibitory effect of metformin on high glucose-induced VSMC proliferation （P < 0.05）. Conclusion High glucose promotes the proliferation of VSMCs, which may be regulated by autophagy. Metformin can upregulate autophagy levels in VSMCs by activating the AMPK/PPAR-γ pathway, thereby inhibiting the abnormal proliferation of VSMCs induced by high sugar.
- Metformin,
- High glucose,
- Autophagy,
- Vascular smooth muscle cell,
- Proliferation

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References(24)

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