Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells

Yelin ZHANG; Liya MA; Xuhui PENG; Hefeng YANG; Rui SHE

doi:10.12259/j.issn.2095-610X.S20231028

Volume 44 Issue 10

Oct. 2023

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Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells[J]. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028

Citation:

Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells[J]. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028

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Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells

doi: 10.12259/j.issn.2095-610X.S20231028

Yelin ZHANG^{1, 2},
Liya MA^{1, 2},
Xuhui PENG³,
Hefeng YANG^{1, 2},
Rui SHE^{1, 2, 4
,
,}

1.
School of Stomatology，Kunming Medical University，Kunming Yunnan 650500
2.
Yunnan Key Laboratory of Stomatology，Kunming Yunnan 650500
3.
Yongshan County People’s Hospital，Zhaotong Yunnan 657300
4.
Dept. of Endodontology，The Affiliated Stomatology Hospital of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-08-13
Available Online: 2023-09-12
Publish Date: 2023-10-25

Abstract

Abstract

Objective To investigate the effect of hsa-let-7a-5p on the proliferation and apoptosis of periodontal ligament stem cells （PDLSCs）. Methods let-7a-5p mimics, mimics nc, let-7a-5p inhibitor, and inhibitor nc were transfected into PDLSCs, and transfection efficiency was measured by RT-qPCR. The effects of let-7a-5p on PDLSCs proliferation were evaluated by CCK-8, cell cycle, and EdU experiments, while the apoptosis rate of PDLSCs was detected by apoptosis experiments. Results let-7a-5p was successfully transfected into PDLSCs. let-7a-5p mimics inhibited cell proliferation （P < 0.05） and promoted cell apoptosis of PDLSCs （P < 0.01）. let-7a-5p inhibitor promoted cell proliferation （P < 0.05） and inhibited cell apoptosis of PDLSCs （P < 0.01）. Conclusion hsa-let-7a-5p can inhibit the proliferation of PDLSCs and promote the apoptosis of PDLSCs.
- hsa-let-7a-5p,
- microRNA,
- Periodontal ligament stem cell,
- Proliferation,
- Apoptosis

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References(31)

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