Predictive Value of Peripheral Blood Septin9 Gene Methylation Assay in Colorectal Adenoma

Juqing DENG; Lu YU; Liqiong LIAO; Zhongcun YANG

doi:10.12259/j.issn.2095-610X.S20240323

Volume 45 Issue 3

Mar. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(3): 151-156

Juqing DENG, Lu YU, Liqiong LIAO, Zhongcun YANG. Predictive Value of Peripheral Blood Septin9 Gene Methylation Assay in Colorectal Adenoma[J]. Journal of Kunming Medical University, 2024, 45(3): 151-156. doi: 10.12259/j.issn.2095-610X.S20240323

Citation:

Juqing DENG, Lu YU, Liqiong LIAO, Zhongcun YANG. Predictive Value of Peripheral Blood Septin9 Gene Methylation Assay in Colorectal Adenoma[J]. Journal of Kunming Medical University, 2024, 45(3): 151-156. doi: 10.12259/j.issn.2095-610X.S20240323

Citation:

PDF( 2226 KB)

Predictive Value of Peripheral Blood Septin9 Gene Methylation Assay in Colorectal Adenoma

doi: 10.12259/j.issn.2095-610X.S20240323

1.
Dept. of Pathology，The 1st People’s Hospital of Kunming City， Kunming Yunnan 650011，China

Received Date: 2023-11-20
Available Online: 2024-03-13
Publish Date: 2024-03-30

Abstract

Abstract

Objective To investigate the predictive significance of Septin9 gene methylation（mSEPT9） in peripheral blood in the diagnosis of colorectal adenoma. Methods A total of 52 subjects were included, 31 patients with colorectal adenomas were collected as the experimental group in the Department of Pathology and 21 subjects with negative colonoscopy in the gastroenterology outpatient clinic were used as the control group from October 2020 to May 2022. mSEPT9 was detected in the two groups, and the results of CEA level in peripheral blood were collected. All the results were statistically analyzed using the Receiver Operating Characteristic（ROC）. Results The area under the curve（area of the ROC: AUC）of mSEPT9detectionto predict the development of adenoma was 0.7205（P < 0.05）, And the cut-off value（CT value） was 39.55, the corresponding sensitivity was 90.91% and the specificity was 56.67%. The AUC of CEA detection for predicting adenoma was 0.5333（P > 0.05）. Conclusions The detection of mSEPT9 is better than that of CEA tumor marker detection in peripheral blood for screening colorectal adenomas with good sensitivity and relative specificity. Invasive colonoscopy for people with positive mSEPT9 screening results can be easier to accept by the general population.
- Septin9 Gene,
- Methylation detection,
- Colorectal adenoma

FullText(HTML)

References(15)

References

[1]	Wild C P, Weiderpass E, Stewart B W. World cancer report: Cancer research for cancer prevention [EB/OL]. （2020-06-20）. https://publications.iarc.fr/586
[2]	E S Lander. Comprehensive molecular characterization of human colon and rectal cancer[J]. Nature,2012,487(7407):330-337. doi: 10.1038/nature11252
[3]	黄丹朱雄增盛伟琪. 2019版《WHO消化系统肿瘤分类》胃肠上皮性肿瘤部分解读[J]. 2020, 49（3）: 209-213.
[4]	Sun J,Zheng M Y,Li Y W,et al. Structure and function of Septin 9 and its role in human malignant tumors[J]. World J Gastrointest Oncol,2020,12(6):619-631. doi: 10.4251/wjgo.v12.i6.619
[5]	Kang Q,Jin P,Yang L,et al. Significance of Septin9 gene methylation detection of plasma circulation DNA in colorectal cancer screening[J]. Zhonghua Yi Xue Za Zhi,2014,94(48):3839-3841.
[6]	Loomans-kropp H A,Song Y,Gala M,et al. Methylated septin9 (mSEPT9): A promising blood-based biomarker for the detection and screening of early-onset colorectal cancer[J]. Cancer Res Commun,2022,2(2):90-98. doi: 10.1158/2767-9764.CRC-21-0142
[7]	Lu D C,Zhang Q F,Li L,et al. Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: A multicenter study[J]. BMC Gastroenterol,2022,22(1):232. doi: 10.1186/s12876-022-02313-x
[8]	Warren J D, XiongW, Bunker A M, et al. Septin 9 methylated DNA is a sensitive and specific blood test for colorectal cancer [J]. BMC Med, 2011, 14: 9: 133.
[9]	Church T R,Wandell M,Lofton-day C,et al. Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer[J]. Gut,2014,63(2):317-325. doi: 10.1136/gutjnl-2012-304149
[10]	吴东,杨红. 外周血甲基化Septin9基因联合粪便免疫化学试验对专科门诊患者结直肠癌和腺瘤的筛查[J]. 中华消化杂志,2016,36(2):107-112. doi: 10.3760/cma.j.issn.0254-1432.2016.02.007
[11]	Ma Z Y,Chan C S Y,Lau K S,et al. Application of droplet digital polymerase chain reaction of plasma methylated septin 9 on detection and early monitoring of colorectal cancer[J]. Sci Rep,2021,11(1):23446. doi: 10.1038/s41598-021-02879-8
[12]	Zhang L,Yang X Y,Zhang T L,et al. Noninvasive detection of esophageal cancer by the combination of mSEPT9 and SNCG[J]. Genet Test Mol Biomarkers,2022,26(1):8-16. doi: 10.1089/gtmb.2021.0089
[13]	Liang Q,Huang Z,Xu Q,et al. A newly developed Septin9 gene methylation assay dectecting double DNA strands for screening of colorectal cancer[J]. Cancer Res,2018,78(13):3329.
[14]	Xu F,Yu S S,Han J Y,et al. Detection of circulating tumor DNA methylation in diagnosis of colorectal cancer[J]. Clin Transl Gastroenterol,2021,12(8):e00386. doi: 10.14309/ctg.0000000000000386
[15]	Mo S B,Wang H,Han L Y,et al. Fecal multidimensional assay for non-invasive detection of colorectal cancer: Fecal immunochemical test,stool DNA mutation,methylation,and intestinal bacteria analysis[J]. Front Oncol,2021,36(2):643136. doi: 10.3389/fonc.2021.643136

Relative Articles(20)

Relative Articles

[1]	Dongping ZHU, Haifeng LI, Junfei FENG, Qiuheng TANG, Jing LENG. Value of SHOX2 and RASSF1A Gene Methylation in Alveolar Lavage Fluid in Patients with Pulmonary Nodules or Masses in the Diagnosis of Lung Cancer. Journal of Kunming Medical University, 2024, 45(3): 106-111. doi: 10.12259/j.issn.2095-610X.S20240316
[2]	Hua LIU, Wanyuan YUE, Shuai SHAO, Jiaping SUN, Ying YANG, Xiaoming DAI. Changes of Genebody Methylation in Lingual Carcinogenesis. Journal of Kunming Medical University, 2024, 45(7): 23-29. doi: 10.12259/j.issn.2095-610X.S20240704
[3]	Wensa YIN, Yumei LU, Jinlian TONG, shengjie NIE, Ye RUAN. Research Progress of DNA Methylation and Schizophrenia. Journal of Kunming Medical University, 2023, 44(8): 145-150. doi: 10.12259/j.issn.2095-610X.S20230811
[4]	Haimei GOU, Li FANG, Xiaowu ZHONG. Relationship between Mutations of KRAS，NRAS and BRAF Genes and Colorectal Liver Metastases. Journal of Kunming Medical University, 2023, 44(1): 19-24. doi: 10.12259/j.issn.2095-610X.S20230106
[5]	Yuan WANG, Rong LI, Jiaojiao CHEN, Yanli SHEN, Fengdi HU. Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer. Journal of Kunming Medical University, 2023, 44(5): 138-143. doi: 10.12259/j.issn.2095-610X.S20230524
[6]	Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice. Journal of Kunming Medical University, 2022, 43(1): 1-7. doi: 10.12259/j.issn.2095-610X.S20220121
[7]	Chang-xian CHEN, Li-ju ZHANG, Xiao-ming JIANG, Zhen-yong ZHANG, Wei-jun LIU. Advances in Treatment of Colorectal Neuroendocrine Tumors. Journal of Kunming Medical University, 2020, 41(12): 155-159. doi: 10.12259/j.issn.2095-610X.S20201242
[8]	Yang Jin Rong , Ruan Jing Yan , Zeng Yun , Wu Kun , Nie Bo , Wang Xue Jiao . . Journal of Kunming Medical University, 2019, 40(09): 12-17.
[9]	Chen Si Yu , Yang Le , Chen Huang Hong , Fu Shuai , Wu Yong . . Journal of Kunming Medical University, 2018, 39(11): 6-10.
[10]	Xiang Li Feng , Wang Qing Qing , Li Ming Ying , Zeng Peng , Li Yong Gang . A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos. Journal of Kunming Medical University, 2018, 39(06): 101-104.
[11]	Wu Gang . HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation. Journal of Kunming Medical University, 2017, 38(11): 5-8.
[12]	Zhao Fei , Jiang Yan Ling , Zhang Shou Xun , Xia Sheng , Bao Jian Jun , Zhang Nin , Yang Xiao Pei , Zhong Shu Rong . Pyrosequencing Detection Pass Ratio for DNA Methylation. Journal of Kunming Medical University, 2017, 38(10): 27-31.
[13]	Yang Xiao Pei , Jiang Yan Ling , Ruan Ye , Zhang Shou Xun , Zhao Fei , Zhang Nin , Xia Sheng , Zhong Shu Rong . Progress on DNA Methylation of OPRM1 Gene in Substance Dependence. Journal of Kunming Medical University, 2017, 38(01): 125-128.
[14]	Yue Wan Yuan . . Journal of Kunming Medical University,
[15]	Zhang Ming Qian . . Journal of Kunming Medical University,
[16]	Di Yu Qiang . . Journal of Kunming Medical University,
[17]	He Zhen Xin . . Journal of Kunming Medical University,
[18]	Yan Ting . . Journal of Kunming Medical University,
[19]	Du Xing Hua . . Journal of Kunming Medical University,
[20]	Yin Liang . . Journal of Kunming Medical University,