Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice

Jun XIA; Weisi LI; Wenjie ZHANG; Zongze QI

doi:10.12259/j.issn.2095-610X.S20240605

Volume 45 Issue 6

Jul. 2024

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Jun XIA, Weisi LI, Wenjie ZHANG, Zongze QI. Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice[J]. Journal of Kunming Medical University, 2024, 45(6): 36-43. doi: 10.12259/j.issn.2095-610X.S20240605

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Jun XIA, Weisi LI, Wenjie ZHANG, Zongze QI. Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice[J]. Journal of Kunming Medical University, 2024, 45(6): 36-43. doi: 10.12259/j.issn.2095-610X.S20240605

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Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice

doi: 10.12259/j.issn.2095-610X.S20240605

1.
Dept. of Abdominal Surgery，Guiqian International General Hospital，Guiyang Guizhou 550000
2.
Dept. of Hepatobiliary Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650500
3.
Dept. of General Surgery，The 1st People’s Hospital of Xundian County，Xundian Yunnan 650000，China

Received Date: 2023-11-03
Available Online: 2024-02-27
Publish Date: 2024-06-25

Abstract

Abstract

Objective To investigate the changes in liver autophagy factor activity in obese mice before and after sleeve gastrectomy（SG）. Methods Male obese mice aged 6-8 weeks were randomly divided into a sham surgery group (Sham group, n=6) and a sleeve gastrectomy surgery group （SG group, n=6）. The daily weight and food intake of the two groups of mice were recorded within one month after surgery, and the fasting blood glucose values at the tip of the mouse tail were measured at 15 and 30 days after surgery. Oral glucose tolerance tests were performed to evaluate glucose absorption; RT-qPCR was used to detect the mRNA expression changes of autophagy factors and glucose transporters at 15 and 30 days after surgery. Results Compared with preoperation, there was no statistically significant difference（P > 0.05） in the weight, daily food intake, and fasting blood glucose values of the sham surgery group at 15 and 30 days after surgery, while the SG group had a decrease in weight, daily food intake, and fasting blood glucose values at 15 and 30 days after surgery and had a statistically significant difference（P < 0.05）; Compared with the sham surgery group, the weight, daily food intake, and fasting blood glucose of the SG group decreased and showed statistical differences（P < 0.05） at 15 and 30 days. The autophagy factor P62 in the SG group mice increased, while the mRNA expression of Beclin-1, LC3I/LC3II, and SGLT1 decreased and showed statistical differences（P < 0.05） at 15 and 30 days after surgery. Conclusion SG can improve obesity and reduce inflammatory mediators and adipokines, further regulating autophagic activity in mouse liver.
- Sleeve-shaped gastrectomy,
- Glucose metabolism in obese mice,
- Autophagy activity

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References(31)

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