Volume 45 Issue 10
Oct.  2024
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Article Navigation >  Journal of Kunming Medical University  > 2024  >  45(10): 17-21
Jiang-pin XU, Jun MA, Nan XIE, Qian WU, Fan CAO, Rong-ji YAN, Hua WANG. Clinical Application Value of Ultrasound in the Diagnosis of Acute Appendicitis[J]. Journal of Kunming Medical University, 2020, 41(11): 78-82. doi: 10.12259/j.issn.2095-610X.S20201118
Citation: Hu ZHANG, Ling LIN, Ailing YANG, Rong SU, Bo HUANG. Establishment of a Smooth Muscle Cell Model for Venous Grafts after Coronary Bypass Surgery[J]. Journal of Kunming Medical University, 2024, 45(10): 17-21. doi: 10.12259/j.issn.2095-610X.S20241003
shu

Establishment of a Smooth Muscle Cell Model for Venous Grafts after Coronary Bypass Surgery

doi: 10.12259/j.issn.2095-610X.S20241003
  • 1.

    Dept. of Geriatric Cardiology

  • 2.

    Operating Room，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

  • Received Date: 2024-04-08
    Available Online: 2024-10-21
  • Publish Date: 2024-10-31
    Abstract
  •   Objective  To explore the establishment of in vitro model of coronary artery bypass venous bridge in coronary artery bypass graft （CABG）.   Methods  Rat venous smooth muscle cells were cultured in vitro and divided into 4 groups. The cells were stretched by cell stretching device in order to simulate the mechanical environment of vein bridge. The changes of cell morphology were observed by CCK-8 detection, cell activity was observed by Edu staining, and cell migration ability was observed by scratch test.   Results  Compared to the normal control group, 5% tensile stress had no significant effect on cell viability （P > 0.05）; 10% tensile stress significantly enhanced cell vitality （P < 0.05）; while 15% tensile stress significantly suppressed cell vitality （P < 0.01）. In terms of cell proliferation, 5% tensile stress showed no significant effect compared to the normal control group （P > 0.05）; 10% tensile stress significantly promoted cell proliferation （P < 0.01）; and 15% tensile stress significantly inhibited cell proliferation （P < 0.05）. Compared to the normal group, 5% tensile stress had no obvious impact on cell migration capability （P > 0.05）; 10% tensile stress promoted cell migration ability （P < 0.05）; and 15% tensile stress significantly inhibited cell migration ability （P < 0.05）.  Conclusion  Under establishment the parameters of 1 Hz frequency and 10% stretch deformation, the biological behavior of vascular smooth muscle cells is consistent with that of clinical stenosis-restenosis cells.
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    • References(15)
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