A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

Yiting TAO; Ge BAI; Dan YU; Zhu ZHOU; Miao LI

doi:10.12259/j.issn.2095-610X.S20241204

Volume 45 Issue 12

Dec. 2024

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Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University, 2024, 45(12): 28-34. doi: 10.12259/j.issn.2095-610X.S20241204

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Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University, 2024, 45(12): 28-34. doi: 10.12259/j.issn.2095-610X.S20241204

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A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

doi: 10.12259/j.issn.2095-610X.S20241204

Yiting TAO¹,
Ge BAI¹,
Dan YU²,
Zhu ZHOU^{1
,
,},
Miao LI^{3
,
,}

1.
The 1st Department of Nephrology
2.
Center of Clinical Pharmacy，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China
3.
Nephrology Department of the First People's Hospital of Qujing, Qujing Yunnan 655000，China

Received Date: 2024-04-07
Available Online: 2024-11-23
Publish Date: 2024-12-31

Abstract

Abstract

Objective To investigate the stability of the two kinds of rat models of chronic renal failure, aiming to provide a basis for establishing a feasible and stable animal model of chronic renal failure. Methods The rat model of chronic renal failure was established by unilateral nephrectomy + adenine gavage + high-phosphorus diet and high-phosphorus + adenine diet, respectively. Erythrocyte and hemoglobin levels, serum urea and creatinine levels were detected at the end of modeling and 6 weeks after modeling, and histopathological examinations of kidney and bone were performed. The tubulointerstitial fibrosis index （TBI） and the percentage of collagen fiber area in renal tissue were calculated. Results At the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 1（unilateral nephrectomy + adenine gavage + high-phosphorus） were significantly decreased（P < 0.05）, the serum urea and creatinine were significantly increased（P < 0.05）, TBI and the percentage of collagen fiber area in kidney tissue were slightly increased（P < 0.05）, and the femur lesions were significantly increased. 6 weeks after modeling, compared with the normal group, the erythrocyte and hemoglobin in model group 1 showed a slight decrease （P < 0.05）, while there was no significant difference in serum urea and creatinine levels. The TBI and collagen fiber area percentage in renal tissue showed a slight increase （P < 0.05）, and there was no obvious change in femoral lesions. At the end of the modeling, the erythrocyte and hemoglobin in the model group 2 （high-phosphorus + adenine diet） were significantly decreased compared with the normal group（P < 0.05）, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased（P < 0.05）, and the lesions of femur were obvious. 6 weeks after the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 2 were significantly decreased（P < 0.05）, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased（P < 0.05）, and the lesions of femur were obvious. 6 weeks after the end of modeling, compared with the model group 1, the erythrocyte and hemoglobin in the model group 2 were significantly decreased（P < 0.05）, while the serum urea, creatinine, TBI, the collagen fiber area percentage of kidney tissue and femur lesions were significantly increased（P < 0.05）. Conclusions A stable rat model of chronic renal failure can be established by high phosphorus + adenine diet.
- Rat,
- Chronic renal failure,
- Adenine,
- Stability

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References(17)

References

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