Semi-automatic Isolation and Purification of Adult Pig Islet Cells

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Semi-automatic Isolation and Purification of Adult Pig Islet Cells[J]. Journal of Kunming Medical University, 2011, 32(10).

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Semi-automatic Isolation and Purification of Adult Pig Islet Cells[J]. Journal of Kunming Medical University, 2011, 32(10).

Semi-automatic Isolation and Purification of Adult Pig Islet Cells[J]. Journal of Kunming Medical University, 2011, 32(10).

Citation:

Semi-automatic Isolation and Purification of Adult Pig Islet Cells[J]. Journal of Kunming Medical University, 2011, 32(10).

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Semi-automatic Isolation and Purification of Adult Pig Islet Cells

Abstract

Abstract

Objective To establish effective method for large-scale purification of islet cells from pig pancreas.Methods Pig pancreas tissue was digested with collagenase P through continuous infusion.Pig islets was purified by Ficoll discontinuous density gradient centrifugation medium.Dithizone(DTZ)staining was used to observe the morphology of islet cells clusters(ICCS),to count islet equivalent IEQ,and to detect the purity of the islet.AO-PI staining was used to detect islet viability and the islet function assess...
- Diabetes,
- Xenogeneic cell transplantation,
- Islet,
- Isolation,
- Purification

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