Detection of Periodontopathogens in Subgingival Plaque of Patients with Coronary Heart Disease and Periodontitis

［Abstract］Objective　To investigate the prevalence and distribution of periodontopathogens in the subgingival plaque of patients with coronary heart disease（CHD）and periodontitis．Methods　50 subgingival plaque samples of patients with CP and 50 subgingival plaque samples of patients with CP and CHD were collected and their bacteria DNA was extracted．Six periodontal pathogens including Porphyromonas gingivalis（Pg）， Prevotella intermedia（Pi），Fusobacterium nucleatum（Fn），Actinobacillus actinomycetemcomitans（Aa）， Tannerella forsythensis（Tf）and Treponema denticola（Td） were detected by the Nested polymerase chain reaction（Nested PCR）．Results　In the CP group，the rates of periodontal bacteria were Pg 98%，Pi 92%，Fn 88%，Aa 6%，Tf 92% and Td 98%；In the CP combined with CHD group ，the rates of periodontal bacteria were Pg 100%，Pi 94%，Fn 80%，Aa 14%，Tf 96% and Td 98%．Conclusion　The distribution of putative periodontopathogens Pg，Pi，Fn，Aa，Tf and Td is similar in the CP group and CP combined with CHD group