Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model

Hou Yun He; Zhang Hao Xin; Wang Yi Yu; Su Yan Mei; Gu Dan Shan; Lin Shao Fang; Yang Shi Kun; Li Shu De

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Article Navigation > Journal of Kunming Medical University > 2018 > 39(04): 27-33

Hou Yun He , Zhang Hao Xin , Wang Yi Yu , Su Yan Mei , Gu Dan Shan , Lin Shao Fang , Yang Shi Kun , Li Shu De . Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model[J]. Journal of Kunming Medical University, 2018, 39(04): 27-33.

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Hou Yun He , Zhang Hao Xin , Wang Yi Yu , Su Yan Mei , Gu Dan Shan , Lin Shao Fang , Yang Shi Kun , Li Shu De . Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model[J]. Journal of Kunming Medical University, 2018, 39(04): 27-33.

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Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB046, 2017FE467); 云南省教育厅科学研究基金资助项目 (2017YJS076); 云南省大学生创新创业训练计划基金资助项目 (2017); 昆明医科大学大学生创新性实验计划基金资助项目 (2017) ;昆明医科大学硕士研究生创新基金资助项目 (2017S054);

Received Date: 2017-08-21

Abstract

Abstract

Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine serum triglyceride and total cholesterol in the fasting state. HE staining was used to detect the morphological changes of the liver, and lipidosis of the liver tissue was observed by the oil red "o" staining in the control group and model control group. The serous concentration of homocysteine wasmeasured in every group by ELISA. Immunohistochemistry and Western blot were used to detect the expression levels of methylenetetrahydrofolate reductase (MTHFR) and cystathionine synthetase (CBS) in liver tissues. Results The concentrations of triglyceride and total cholesterol in the model control group were disordered compared with the control group (P<0.05) , meanwhile the morphology of the liver tissue was disordered and lipidosis was obvious in the model control group. So those results indicated the NAFLD model was successful. The concentration of homocysteine in the model control group was increased and decreased by Ginsenoside Rg1 treating (P<0.05) . The protein expressions of MTHER and CBS in model control group were lower compared with the control group by immunohistochemistry and Western blot (P<0.05) . The protein expressions ofMTHFR and CBS in the ginsenoside Rg1 treatment group were higher compared with the model control group by immunohistochemistry and Western blot (P<0.05) . The results have dose-dependent. Conclusion Ginsenoside Rg1 is possible to increase the protein expressions of MTHFR and CBS and decrease the concentration of homocysteine in animal models of non-alcoholic fatty liver, thus it can play a protective effect in non-alcoholic fatty liver.
- NAFLD,
- Ginsenoside Rg1,
- Homocysteine,
- MTHFR,
- CBS

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References(17)

References

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