The Effect of Cinnamaldehyde and Vancomycin on Biofilm Inhibition of Methicillin-Resistant Staphylococcus Aureus

Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus (MRSA) . Me thods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard strain ATCC25923 and other 5 strains from patient samples, and crystal violet semi-quantitative method was applied to detect the biofilm formation. The minimum inhibitory concentration (MIC) of ATCCA strain and typical MRSA strain was determined using triphenyl tetrazolium chloride (TTC) method. Micrdilution checkerboard techniques was adopted to determine the MIC of drugs combination. Re s ults The biofilm will be maturated in three days;Strain 16187 showed strong capacity of biomilm formation; MICs of standard strain for cinnamaldehyde and vancomycin were 256 μg/m L and 1 μg/m L; MICs of strain 16187 for cinnamaldehyde and vancomycin were 128 μg/m L and 0.5 μg/m L;MIC of standard strain for combination of cinnamaldehyde and vancomycin were 16 μg/m L and 0.006 μg/m L, and 16 μg/m L and 0.03 μg/m L for stain 16187. Comparing MRSA16187 in treated with medicatoin conbired with cinnamaldehyde and vancomycinand single medication the difference of sMIC50 was significant (P<0.05 and P<0.05) , and there was significant difference when we compared sMIC90 (P<0.05 and P<0.05) ;Comparing ATCC25923 treated with medication combined with cinnamaldehyde and vancomycin the difference was of sMIC50 significant (P<0.05 and P<0.05) , and sMIC90 showed the significant difference (P <0.05 and P <0.01) .Conclusion Cinnamaldehyde synergistically enhances the capacity of vancomycin on biofilminhibitory on MRSA.