Molecular Typing and Drug Resistance of Salmonella Typhimurium in Yunnan Province

Tian Yun Ping; Zou Yan Qiu Shuo; Jin Li; Zhao Jiang; Ren Xiang; Yin Jian Wen

Volume 39 Issue 08

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Tian Yun Ping , Zou Yan Qiu Shuo , Jin Li , Zhao Jiang , Ren Xiang , Yin Jian Wen . Molecular Typing and Drug Resistance of Salmonella Typhimurium in Yunnan Province[J]. Journal of Kunming Medical University, 2018, 39(08): 23-27.

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Tian Yun Ping , Zou Yan Qiu Shuo , Jin Li , Zhao Jiang , Ren Xiang , Yin Jian Wen . Molecular Typing and Drug Resistance of Salmonella Typhimurium in Yunnan Province[J]. Journal of Kunming Medical University, 2018, 39(08): 23-27.

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Molecular Typing and Drug Resistance of Salmonella Typhimurium in Yunnan Province

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基金：云南省卫生计生委食品安全保障基金资助项目[ (2012) 659号];

Received Date: 2018-04-19

Abstract

Abstract

Objective To understand the molecular typing and drug resistance of Salmonella typhimurium in Yunnan Province, to provide scientific basis for preventing and controlling diseases caused by Salmonella typhimurium. Methods Molecular typing was carried out according to Salmonella PFGE typing technology published by Pulse Net China. The MIC value of the drug sensitive plate was obtained from the analysis of the instructions, and the results of S, I and R were obtained according to the corresponding standards of CLSI.Results The 68 strains salmonella typhimurium in Yunnan Province showed 53 types of PFGE. The drug resistance rate of the 68 strains of salmonella typhimurium was the highest for the compound sulfonamide (SXT) , 45.59%, and the most sensitive to imipenem (IMP) , among which one strain was resistant to 11 of the14 antibiotics. Conclusion The molecular typing of salmonella typhimuriumin in Yunnan province is diverse.Compound sulfonamide is the most resistant antibiotic of Salmonella typhimurium in this region. Multiple drug resistance of Salmonella typhimurium in this region is atypical.
- Salmonella typhi,
- PFGE,
- Drug resistance

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[1] 朱超, 许学斌.沙门氏菌属血清型诊断[M].上海:同济大学出版社, 2OO9:133-135.
[2]李梦东, 王宇明.实用传染病学[M].第3版.北京人民卫生出版社, 2004:762-769.
[3]许云敏, 杜艳, 单斌, 等.2005-2014年CHINET沙门氏菌属细菌耐药性监测[J].中国感染与化疗杂志, 2016, 16 (3) :294-301.
[4]王丽丽, 徐建国.脉冲场凝胶电泳技术 (PFGE) 在分子分型中的应用现状[J].疾病监测, 2006, 21 (5) :276-279.
[5]李柏生, 柯碧霞, 何冬梅, 等.广东省腹泻病例非伤寒沙门氏菌耐药谱和PFGE分型研究[J].中华微生物学和免疫学杂志, 2012, 32 (6) :542-548.
[6]	[6]TORPDAHL M, SORENSEN G, ETHELBERG S, et al.A regional outbreak of S.typhimurium in Denmark and identification of thesource using MLVA typing[J].Euro Surveill, 2006, 11 (5) :134-136.
[7]	[7]THONG K L, NGEOW Y F, ALTWEGG M, et a1.Molecular analysis of Salmonella typhimurium enteritid is by Trad-field gel electrophoresis and ribo typing[J].J Clin Microbiol, 1995, 33 (5) :1070-1074.
[8]	[8]SOYER Y, ALCAINE S D.SCHOONMAKERBOPP D J, et a1.Trad-field gel electrophoresis diversity of human and bovinec linical Salmonella typhimurium isolates[J].Foodborne Pathog Dis, 2010, 7 (6) :707-717.
[9] 魏仲秋, 常海岭, 李月芳, 等.2012-2014年儿童非伤寒沙门氏菌肠道感染的临床流行病学特征及耐药模式[J].中华儿科杂志, 2016, 54 (7) :489-495.
[10]方欣, 李春.安徽地区腹泻病人粪便标本中沙门氏菌分离及其血清型鉴定[J].中国人兽共患病学报, 2014, 31 (2) :135-138.
[11]王岚, 贾华云, 张红, 等.湖南省食源性沙门氏菌血清型分布及耐药性研究[J].实用预防医学, 2011, 18 (6) :994-997.
[12]张新, 曲梅, 黄瑛, 等.北京市21株鼠伤寒沙门氏菌多重耐药和分子分型研究[J].中华临床医师杂志 (电子版) , 2012, 06 (14) :4477-4479.
[13]赵嘉咏, 黄丽莉, 穆玉姣, 等.2011-2013年河南省鼠伤寒沙门氏菌耐药与分子分型研究[J].中国人兽共患病学报, 2016, 32 (1) :56-60.
[14]梁燕霞, 李小南, 伍绍东.204例儿童感染鼠伤寒沙门氏菌临床分析[J].中国实用医药, 2016, 11 (17) :44-45.
[15]张晶, 张欣强, 侯水平, 等.广州市566株腹泻病例沙门氏菌的血清型分布及耐药性分析[J].实用预防医学, 2016, 23 (5) :616-623.
[16]程春荣, 沈艳丽, 董柯, 等.郑州市2006-2011年腹泻病人沙门氏菌血清型和耐药谱分析[J].中国人兽共患病学报, 2012, 28 (10) :1017-1019.
[17]陈强, 余晓君, 李俏俏, 等.引起儿童腹泻的沙门菌属临床分离株的耐药特点及分子流行病学研究[J].中华检验医学杂志, 2011, 34 (3) :249-253.
[18] 张广业, 汪鹏, 康倩.甘肃省沙门氏菌菌型分布及耐药性监测分析[J].医学信息, 2014, 22 (5) :36-37.
[19]杨劲松, 陈建辉, 林杰, 等.福建省鼠伤寒沙门菌PFGE分子分型和耐药的关联性研究[J].中国人兽共患病学报, 2012, 28 (1) :10-13.
[20]蔡和花, 李桦, 王慧, 等.33例儿童非伤寒沙门菌肠炎临床分析及耐药监测[J].四川生理科学杂志, 2016, 38 (2) :76-79.
[21]程招敏, 蓝锴, 柏彩英, 等.鼠伤寒沙门菌分子分型及耐药性特点[J].国际检验医学杂志, 2016, 37 (12) :1601-1603.
[22]何聪.鼠伤寒沙门菌感染24例临床分析[J].内蒙古中医药, 2010, 29 (21) :16-17.

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2.	颜淑妩，曹赛红，周奇文，李晟，陈帅，陈丽丽. 衡阳市生畜肉及熟肉制品中沙门菌血清型、分子分型及耐药性研究. 实用预防医学. 2023(03): 266-269 .
3.	何玉凤，杨祖顺. 云南省过桥米线加工过程微生物污染监测与分子分型及耐药分析. 疾病预防控制通报. 2023(03): 74-78 .
4.	王慧雯，邹颜秋硕，杨祖顺，田云屏，任翔. 鼠伤寒沙门菌脉冲场凝胶电泳分子分型及耐药分析. 实用预防医学. 2023(08): 932-936 .
5.	邹文燕，王小龙，朱莉勤，张梦寒，崔家瑞，汤全英. 2020年苏州市生畜禽肉与调理肉制品食源性致病菌监测结果分析. 江苏预防医学. 2022(01): 94-96+99 .
6.	曹文婷，沙丹，齐倩倩，马广源，管红霞. 2011至2018年无锡市鼠伤寒沙门菌耐药性分析与分子分型研究. 中华检验医学杂志. 2022(02): 157-163 .
7.	尹建雯，贾森泉，周永明，古文鹏，赵智娴. 健康从业人员中产ESBLs鼠伤寒沙门氏菌的研究. 云南医药. 2022(05): 18-21 .
8.	王尔群，舒玉聪，宋志琴，秦超，黄伟峰. 2014—2019年成都市新都区鼠伤寒沙门菌耐药特性和分子分型研究. 中国食品卫生杂志. 2021(01): 69-74 .
9.	黄海林，李小娟，尹建雯，蒋鸿超. 2017年至2020年儿童感染性腹泻沙门菌和志贺菌分布及药敏结果. 昆明医科大学学报. 2021(07): 83-87 . 本站查看
10.	石红，王毅. 183株沙门菌属细菌的血清型分布、分子分型特征及耐药性分析. 海南医学. 2021(18): 2392-2394 .
11.	王燕梅，霍翔，郑东宇，乔昕，倪云龙. 江苏省2017—2018年监测分离的588株食源性沙门菌血清分型与分子分型. 江苏预防医学. 2021(06): 666-668 .
12.	童锐，陈秀华，刘芸，张颖华，王小光，丁克颖. 上海市闵行区鼠伤寒沙门菌病原学与PFGE分型研究. 中国卫生检验杂志. 2020(02): 136-140 .
13.	周倩，周藜，黄靖宇，向婧姝，张德著. 2015-2017年贵州省食源性蜡样芽孢杆菌脉冲场凝胶电泳分型研究. 职业卫生与病伤. 2019(04): 202-205 .
14.	肖波，陶顺，徐洪琳，李淋，陈曦. 一起鼠伤寒沙门氏菌食物中毒的病原鉴定与同源性分析. 职业卫生与病伤. 2019(05): 319-322 .
15.	周涛，倪文玲，倪刚，徐晋莹，吴春梅，李青，谈星延. 2011～2018年红河州食品中沙门氏菌血清分型及脉冲场凝胶电泳分型研究. 食品安全质量检测学报. 2019(22): 7694-7700 .