Study on the Correlation Between Multiple Drug Resistance of <i>Acinetobacter baumannii</i> with Efflux Pump and Biofilm Formation

Jia WANG; Lei FENG; Yulei ZHENG; Yong YUAN; Yao YAO; Zidang HU; Ni SHENG; Yan YU; Jiawei DING

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Jia WANG, Lei FENG, Yulei ZHENG, Yong YUAN, Yao YAO, Zidang HU, Ni SHENG, Yan YU, Jiawei DING. Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation[J]. Journal of Kunming Medical University.

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Jia WANG, Lei FENG, Yulei ZHENG, Yong YUAN, Yao YAO, Zidang HU, Ni SHENG, Yan YU, Jiawei DING. Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation[J]. Journal of Kunming Medical University.

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Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation

1.
Dept. of Clinical Laboratory ，Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650051
2.
Dept. of Clinical Laboratory，Panlong District People’s Hospital，Kunming Yunnan 650201，China

Received Date: 2024-11-01
Available Online: 2024-11-23

Abstract

Abstract

Objective To investigate the correlation between biofilm gene, efflux pump gene, biofilm forming ability and drug resistance of Acinetobacter baumarmii （AB） isolated from the patients of clinical infectious diseases. Methods A total of 119 non-repeating Acinetobacter baumannii isolates （74 MDR-AB strains and 45 non-multi-drug resistant strains） were collected. The effector pump genes （adeB, adeJ, adeG, adeS and adeR） and biofilm-related genes （bap, ompA, csuA, csuB, csuC, csuD, csuE, abaI, bfmR, bfmS） were detected by PCR. Bacterial biofilm was constructed in vitro with 24-well cell culture plates, and the formation of biofilm was qualitatively observed by crystal violet staining. Results Acinetobacter baumannii was resistant to most of the commonly used antibiotics and sensitive to tigacycline, polymyxin B and minocycline. The overall detection rate of biofilm-related genes was about 80%, and the detection rates of bap, csuB, csuC and csuD genes in multi-drug resistant bacteria were significantly higher than those in non-multi-drug resistant bacteria （P < 0.05）. The detection rate of active effector pump genes was less than 70%, and the detection rate of adeB, adeJ, adeS and adeR genes in multidrug-resistant bacteria was significantly higher than that in non-multidrug-resistant bacteria （P < 0.05）. The adeG carrying rate was opposite. Three multidrug-resistant strains and five non-multidrug-resistant strains did not carry the above efflux pump related genes, and no abeM gene was detected in all strains. The formation of biofilm was observed in 95.80% of strains. Conclusion Acinetobacter baumannii in hospital is mostly multidrug-resistant strains, most of which have strong biofilm forming ability. Multidrug-resistant bacteria have higher biofilm gene and effluent-pump gene carrying rate, which may be involved in the regulation and formation of multidrug resistance.
- Acinetobacter baumannii,
- Multiple drug resistance,
- Efflux pump,
- Biofilm

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References(17)

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