Study on the Correlation Between Multiple Drug Resistance of <i>Acinetobacter baumannii</i> with Efflux Pump and Biofilm Formation

Jia WANG; Lei FENG; Yulei ZHENG; Yong YUAN; Yao YAO; Zidang HU; Ni SHENG; YUYan; Jiawei DING

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Jia WANG, Lei FENG, Yulei ZHENG, Yong YUAN, Yao YAO, Zidang HU, Ni SHENG, YUYan, Jiawei DING. Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation[J]. Journal of Kunming Medical University.

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Jia WANG, Lei FENG, Yulei ZHENG, Yong YUAN, Yao YAO, Zidang HU, Ni SHENG, YUYan, Jiawei DING. Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation[J]. Journal of Kunming Medical University.

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Study on the Correlation Between Multiple Drug Resistance of Acinetobacter baumannii with Efflux Pump and Biofilm Formation

1.
Dept. Of Clinical Laboratory ，Yan'an Hospital Affiliated to Kunming Medical University，kunming，yunnan，650051
2.
Dept. Of Clinical Laboratory，Panlong District People's Hospital，kunming yunnan，650000，China

Received Date: 2024-11-01

Abstract

Abstract

Objective To investigate the correlation between biofilm gene, efflux pump gene, biofilm forming ability and drug resistance of Acinetobacter baumarmii （AB） isolated from the patients of clinical infectious diseases. Methods A total of 119 non-repeating Acinetobacter baumannii isolates （74 MDR-AB strains and 45 non-multi-drug resistant strains） were collected. The effector pump genes （adeB, adeJ, adeG, adeS and adeR） and biofilm-related genes （bap, ompA, csuA, csuB, csuC, csuD, csuE, abaI, bfmR, bfmS） were detected by PCR. Bacterial biofilm was constructed in vitro with 24-well cell culture plates, and the formation of biofilm was qualitatively observed by crystal violet staining. Results Acinetobacter baumannii was resistant to most of the commonly used antibiotics and sensitive to tigacycline, polymyxin B and minocycline. The overall detection rate of biofilm-related genes was about 80%, and the detection rates of bap, csuB, csuC and csuD genes in multi-drug resistant bacteria were significantly higher than those in non-multi-drug resistant bacteria （P < 0.05）. The detection rate of active effector pump genes was less than 70%, and the detection rate of adeB, adeJ, adeS and adeR genes in multidrug-resistant bacteria was significantly higher than that in non-multidrug-resistant bacteria （P < 0.05）. The adeG carrying rate was opposite. Three multidrug-resistant strains and five non-multidrug-resistant strains did not carry the above efflux pump related genes, and no abeM gene was detected in all strains. The formation of biofilm was observed in 95.80% of strains. Conclusion Acinetobacter baumannii in hospital is mostly multidrug-resistant strains, most of which have strong biofilm forming ability. Multidrug-resistant bacteria have higher biofilm gene and effluent-pump gene carrying rate, which may be involved in the regulation and formation of multidrug resistance.
- Acinetobacter baumannii,
- Multiple drug resistance,
- Efflux pump,
- Biofilm

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References(20)

References

[1]	Pulingam T,Parumasivam T,Gazzali A M,et al. Antimicrobial resistance: Prevalence,economic burden,mechanisms of resistance and strategies to overcome[J]. European Journal of Pharmaceutical Sciences,2022,170:106103. doi: 10.1016/j.ejps.2021.106103
[2]	Vrancianu C O,Gheorghe I,Czobor I B,et al. Antibiotic resistance profiles,molecular mechanisms and innovative treatment strategies of Acinetobacter baumannii[J]. Microorganisms,2020,8(6):935. doi: 10.3390/microorganisms8060935
[3]	Liu G,Qin M. Analysis of the Distribution and Antibiotic Resistance of Pathogens Causing Infections in Hospitals from 2017 to 2019[J]. Evid-based compl alt,2022,9(16):211-218
[4]	袁茂冉,葛宏华,马金鸣,等. 鲍曼不动杆菌外排泵介导多药耐药性[J]. 中国生物化学与分子生物学报,2020,36(11):1295-1302.
[5]	蔺飞,袁明勇,凌保东,等. 鲍曼不动杆菌生物膜相关基因研究[J]. 中华实验和临床感染病杂志(电子版),2021,15(2):129-132.
[6]	牛付轩,临床分离鲍曼不动杆菌生物膜形成能力与耐药性关系研究[D]. 镇江市: 江苏大学,2019.
[7]	谢思露,临床鲍曼不动杆菌生物膜形成与耐药能力的相关性研究[D]. 成都市: 成都中医药大学,2020.
[8]	周亚玲,阴晴,王中新,等. 鲍曼不动杆菌碳青霉烯酶和RND外排泵相关耐药基因的研究[J]. 国际检验医学杂志,2021,42(21):2635-2639.
[9]	Wang L,Huang Y,Yin G,et al. Antimicrobial activities of Asian ginseng,American ginseng,and notoginseng[J]. Phytother Res,2020,34(6):1226-1236. doi: 10.1002/ptr.6605
[10]	王佳,曹向红,彭传梅,等. 昆明地区多重耐药鲍曼不动杆菌耐药特征及同源性分析[J]. 国际检验医学杂志,2022,43(21):2596-2600+2606.
[11]	Dan N,Yue H,Zhou C,et al. Outer membrane protein A (OmpA) as a potential therapeutic target for Acinetobacter baumannii infection[J]. Journal of Biomedical Science,2020,27(8):1679-1702.
[12]	Li X,Song Y,Wang L,et al. A Potential Combination Therapy of Berberine Hydrochloride With Antibiotics Against Multidrug-Resistant Acinetobacter baumannii[J]. Front Cell Infect Microbiol,2021,11(2):12-20.
[13]	Alfonso Soler-Bistué. Restriction–methylation systems regulate transformation in Acinetobacter baumannii[J]. Trends in Microbiology,2023,31(9):879-881 doi: 10.1016/j.tim.2023.07.009
[14]	Ning Dong,Yu Zeng,Yao Wang,et al. Distribution and spread of the mobilised RND efflux pump gene cluster tmexCD-toprJ in clinical Gram-negative bacteria: a molecular epidemiological study[J]. Lancet Microbe,2022,3(8):46-56.
[15]	Mariana Castanheira,Rodrigo E. Mendes,and Ana C. Gales,et al. Global Epidemiology and Mechanisms of Resistance of Acinetobacter baumannii-calcoaceticus. Complex Clinical Infectious Diseases 2023,5(1): S166–S178.
[16]	Chyi-Liang Chen,Rajendra Prasad Janapatla,Anna Dudek,et al. Functional characterization and clinical implication of a novel epidemic carbapenem-resistant Acinetobacter baumannii genetic markerInternational[J]. Journal of Antimicrobial Agents,2023,8(61):102-110.
[17]	刘佳婷,大剂量舒巴坦或多粘菌素治疗耐药鲍曼不动杆菌的系统评价及药物不良反应的大数据分析[D]. 泸州市: 西南医科大学 2022
[18]	徐益,王佳,高辉,朱雯梅,等. 《多重耐药鲍曼不动杆菌耐药性及耐药基因检测》[J]. 国际检验医学杂志,2020,41(8):993-995.
[19]	Mariana Castanheira,Rodrigo E. Mendes,AnaC. Gales. Global Epidemiology and Mechanisms of Resistance of Acinetobacter baumannii-calcoaceticus Complex[J]. Clinical Infectious Diseases,2023,76:166-178. doi: 10.1093/cid/ciad109
[20]	刘晓琳,王辉,倪语星,等《M100抗微生物药物敏感性试验执行标准》第33版,2023: 3

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