Protective Effects of Xiyanping Injection on Lipopolysaccharide-induced Acute Lung Injury in Mice

Shaoyan NIE; Susu FAN; Yushan ZHU; Xuerong PENG; Yang WANG; Xuan ZHANG

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Shaoyan NIE, Susu FAN, Yushan ZHU, Xuerong PENG, Yang WANG, Xuan ZHANG. Protective Effects of Xiyanping Injection on Lipopolysaccharide-induced Acute Lung Injury in Mice[J]. Journal of Kunming Medical University.

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Protective Effects of Xiyanping Injection on Lipopolysaccharide-induced Acute Lung Injury in Mice

Shaoyan NIE^{1, 2},
Susu FAN²,
Yushan ZHU²,
Xuerong PENG²,
Yang WANG^{3
,
,},
Xuan ZHANG^{2
,
,}

1.
Dept. of Pharmacy，Yunnan Cancer Hospital/The 3rd Affiliated Hospital of Kunming Medical University/Beijing Cancer Hospital Yunnan Hospital, Kunming Yunnan 650118
2.
School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Natural Products/College of Modern Biomedical Industry, Kunming Medical University, Kunming Yunnan 650500
3.
Dept. of Pharmacy，Yan’an Hospital of Kunming City，Kunming Yunnan 650051，China

Received Date: 2024-05-26
Available Online: 2024-11-09

Abstract

Abstract

Objective To explore the protective effects of Xiyanping injection against lipopolysaccharide （lipopolysaccharide, LPS）-induced acute lung injury （acute lung injury, ALI）in mice, and preliminarily investigate its mechanism. Methods The mice were randomly divided into the normal control group （NS）, ALI model group （LPS）, Xiyanping injection low dosage group （XYP-L, 18.96 mg/kg）, medium dosage group （XYP-M, 37.92 mg/kg）, and high dosage group （XYP-H, 75.83 mg/kg） and dexamethasone positive drug group （DXM, 3.03 mg/kg）, with 20 in each group. Each group was injected intraperitoneally with the corresponding drug, once a day at the same time for 3 consecutive days. the NS and LPS groups were given the same 0.9% sodium chloride injection volume. Except for NS group, LPS was instilled into the trachea to induce the acute lung injury in mice 1 hour after the last administration of the above drugs. The lung tissue samples and broncho alveolar lavage fluid （BALF） samples were collected 24 hours after the intratracheal instillation of LPS. The lung coefficient was calculated. The pathological and morphological changes of lung tissue were observed macroscopically and microscopially. The total cell count in BALF was calculated under the microscope. The concentration of total protein in BALF was measured using the BCA （bicin-choninic acid） method. The content of inflammatory cytokines tumor necrosis factor-α（TNF-α）, interleukin-1β（IL-1β）, and interleukin-8 （IL-8）in BALF was detected using the ELISA （enzyme linked immunosorbent assay） method. The levels of malondialdehyde （MDA） and superoxide dismutase （SOD） in mouse lung tissue were measured using MDA and SOD assay kits. Results Compared with the NS group, the LPS group showed severe pathological damage in the lung tissue, increased lung coefficient, total cell count, total protein concentration, and elevated levels of TNF-α, IL-1β, and IL-8 in BALF （P < 0.01）. The LPS group also exhibited the higher levels of MDA in the lung tissue （P < 0.001）, decreased SOD activity （P < 0.001）. The treatment groups showed the improvements in the lung tissue damage compared to the LPS group. The treatment groups exhibited the decreased lung coefficient, total cell count, total protein concentration, TNF-α, IL-1β, and IL-8 levels in BALF （P < 0.05）, reduced MDA content in lung tissue （P < 0.001）, and increased SOD activity （P < 0.05）. Conclusions Xiyanping injection has a certain protective effect on LPS-induced ALI in mice, and its mechanism is related to anti-inflammatory and antioxidant effects. It can alleviate the lung inflammation through inhibiting the release of downstream inflammatory factors and oxidative stress in the lung tissue caused by LPS through antioxidant effects.
- Xiyanping injection,
- Lipopolysaccharide,
- Network pharmacology,
- Acute lung injury,
- Inflammation oxidative stress

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References(13)

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