A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

Yiting TAO; Ge BAI; Dan YU; Zhu ZHOU; Miao LI

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 >

Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

Citation:

Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

Citation:

Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

PDF( 5725 KB)

A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

Yiting TAO¹,
Ge BAI¹,
Dan YU²,
Zhu ZHOU^{1
,
,},
Miao LI^{3
,
,}

1.
The 1st Department of Nephrology
2.
Center of Clinical Pharmacy，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China
3.
Nephrology Department of the First People's Hospital of Qujing, Qujing Yunnan 655000，China

Received Date: 2024-04-07
Available Online: 2024-11-23

Abstract

Abstract

Objective To investigate the stability of the two kinds of rat models of chronic renal failure, aiming to provide a basis for establishing a feasible and stable animal model of chronic renal failure. Methods The rat model of chronic renal failure was established by unilateral nephrectomy + adenine gavage + high-phosphorus diet and high-phosphorus + adenine diet, respectively. Erythrocyte and hemoglobin levels, serum urea and creatinine levels were detected at the end of modeling and 6 weeks after modeling, and histopathological examinations of kidney and bone were performed. The tubulointerstitial fibrosis index （TBI） and the percentage of collagen fiber area in renal tissue were calculated. Results At the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 1（unilateral nephrectomy + adenine gavage + high-phosphorus） were significantly decreased（P < 0.05）, the serum urea and creatinine were significantly increased（P < 0.05）, TBI and the percentage of collagen fiber area in kidney tissue were slightly increased（P < 0.05）, and the femur lesions were significantly increased. 6 weeks after modeling, compared with the normal group, the erythrocyte and hemoglobin in model group 1 showed a slight decrease （P < 0.05）, while there was no significant difference in serum urea and creatinine levels. The TBI and collagen fiber area percentage in renal tissue showed a slight increase （P < 0.05）, and there was no obvious change in femoral lesions. At the end of the modeling, the erythrocyte and hemoglobin in the model group 2 （high-phosphorus + adenine diet） were significantly decreased compared with the normal group（P < 0.05）, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased（P < 0.05）, and the lesions of femur were obvious. 6 weeks after the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 2 were significantly decreased（P < 0.05）, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased（P < 0.05）, and the lesions of femur were obvious. 6 weeks after the end of modeling, compared with the model group 1, the erythrocyte and hemoglobin in the model group 2 were significantly decreased（P < 0.05）, while the serum urea, creatinine, TBI, the collagen fiber area percentage of kidney tissue and femur lesions were significantly increased（P < 0.05）. Conclusions A stable rat model of chronic renal failure can be established by high phosphorus + adenine diet.
- Rat,
- Chronic renal failure,
- Adenine,
- Stability

FullText(HTML)

References(17)

References

[1]	Ku E, Del Vecchio L, Eckardt K U, et al. Novel anemia therapies in chronic kidney disease: Conclusions from a Kidney Disease: Improving Global Outcomes (KDIGO) Controversies Conference[J]. Kidney Int,2023,104(4):655-680.
[2]	Ku E,Del Vecchio L,Eckardt K U,et al. Novel anemia therapies in chronic kidney disease: conclusions from a Kidney Disease: Improving Global Outcomes (KDIGO) Controversies Conference[J]. Kidney Int,2023,104(4):655-680. doi: 10.1016/j.kint.2023.05.009
[3]	Kaur R,Singh R. Mechanistic insights into CKD-MBD-related vascular calcification and its clinical implications[J]. Life Sci,2022,311(Pt B): 121148.
[4]	GBD Chronic Kidney Disease Collaboration. Global,regional,and national burden of chronic kidney disease,1990-2017: a systematic analysis for the global burden of disease study 2017[J]. Lancet,2020,395(10225):709-733. doi: 10.1016/S0140-6736(20)30045-3
[5]	Ruiz-Ortega M,Rayego-Mateos S,Lamas S,et al. Targeting the progression of chronic kidney disease[J]. Nat Rev Nephrol,2020,16(5):269-288. doi: 10.1038/s41581-019-0248-y
[6]	Adam R J,Williams A C,Kriegel A J. Comparison of the surgical resection and infarct 5/6 nephrectomy rat models of chronic kidney disease[J]. Am J Physiol Renal Physiol,2022,322(6):F639-f654. doi: 10.1152/ajprenal.00398.2021
[7]	Atteia H H,Alamri E S,Sirag N,et al. Soluble guanylate cyclase agonist,isoliquiritigenin attenuates renal damage and aortic calcification in a rat model of chronic kidney failure[J]. Life Sci,2023,317:121460. doi: 10.1016/j.lfs.2023.121460
[8]	Diwan V,Mistry A,Gobe G,et al. Adenine-induced chronic kidney and cardiovascular damage in rats[J]. J Pharmacol Toxicol Methods,2013,68(2):197-207. doi: 10.1016/j.vascn.2013.05.006
[9]	Yazgan B,Avcı F,Memi G,et al. Inflammatory response and matrix metalloproteinases in chronic kidney failure: Modulation by adropin and spexin[J]. Exp Biol Med (Maywood),2021,246(17):1917-1927.
[10]	Radford M G,Jr, Donadio J V,Jr, Bergstralh E J,et al. Predicting renal outcome in IgA nephropathy[J]. J Am Soc Nephrol,1997,8(2): 199-207.
[11]	Zhu J,Chen A,Gao J,et al. Diffusion-weighted,intravoxel incoherent motion,and diffusion kurtosis tensor MR imaging in chronic kidney diseases: Correlations with histology[J]. Magn Reson Imaging,2024,106:1-7. doi: 10.1016/j.mri.2023.07.002
[12]	Yokozawa T,Zheng P D,Oura H,et al. Animal model of adenine-induced chronic renal failure in rats[J]. Nephron,1986,44(3):230-234.
[13]	Diwan V,Brown L,Gobe G C. Adenine-induced chronic kidney disease in rats[J]. Nephrology (Carlton),2018,23(1):5-11. doi: 10.1111/nep.13180
[14]	Levey A S,Stevens L A,Coresh J. Conceptual model of CKD: Applications and implications[J]. Am J Kidney Dis,2009,53(3 Suppl 3): S4-16.
[15]	López-Novoa J M,Rodríguez-Peña A B,Ortiz A,et al. Etiopathology of chronic tubular,glomerular and renovascular nephropathies: clinical implications[J]. J Transl Med,2011,9:13.
[16]	Kim K,Anderson E M,Thome T,et al. Skeletal myopathy in CKD: A comparison of adenine-induced nephropathy and 5/6 nephrectomy models in mice[J]. Am J Physiol Renal Physiol,2021,321(1):F106-f119. doi: 10.1152/ajprenal.00117.2021
[17]	Barratt J,Andric B,Tataradze A,et al. Roxadustat for the treatment of anaemia in chronic kidney disease patients not on dialysis: A Phase 3,randomized,open-label,active-controlled study (DOLOMITES)[J]. Nephrol Dial Transplant,2021,36(9):1616-1628. doi: 10.1093/ndt/gfab191

Relative Articles(20)

Relative Articles

[1]	Piao LI, Xiaolin LIU, Ai YANG, Qian ZHANG, Weidong LU. Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20220307
[2]	Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210403
[3]	Zhen CHEN, Li-ping GAO. Changes and Clinical Significance of Serum Cys C, β 2-MG, MCP-1 and Ang Ⅱ Levels in Patients with Chronic Renal Failure. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210204
[4]	Zhu Wen Yong , Wang Ming Qing , Song Shao Hui , Li Shuang Li , Hu Wen Zhu , Liao Guo Yang . . Journal of Kunming Medical University,
[5]	Jiang Hong Ying , Zhang Xi , Zhang Le Hang , Zeng Yi , Peng Wen Fang , Bai Yi Hua . . Journal of Kunming Medical University,
[6]	Lan Ping , Ye Hong Yan , Fu Wei , Fan Yu Chao , Zhao Xiang Yue , Lu Wei Dong . . Journal of Kunming Medical University,
[7]	Huang Wei Min . . Journal of Kunming Medical University,
[8]	Zeng Yu Jian . . Journal of Kunming Medical University,
[9]	Li Hong Li . . Journal of Kunming Medical University,
[10]	Xu Jian . . Journal of Kunming Medical University,
[11]	Tong Zong Wu . . Journal of Kunming Medical University,
[12]	Zhao Chun Mei . . Journal of Kunming Medical University,
[13]	. Effects of Exercise Training on Glucose Intolerance in Rats with Type 2 Diabetes Induced by Alloxan. Journal of Kunming Medical University,
[14]	. Clinical Analysis of 65 Patients with Chronic Renal Failure and Pulmonary Infection. Journal of Kunming Medical University,
[15]	Xu Jing . Study of Heterografting of Microliver Slice in Rats with Fulminant Hepatic Failure. Journal of Kunming Medical University,
[16]	. The Influence of Turbidity-reducing Granule on Collagen Ⅰ in Kidneys of Rats with Chronic Renal Failure. Journal of Kunming Medical University,
[17]	Tang Mao Dan . . Journal of Kunming Medical University,
[18]	Yin Lu Wei . . Journal of Kunming Medical University,
[19]	Cheng Jian Jun . . Journal of Kunming Medical University,
[20]	Wang Zhao Jun . . Journal of Kunming Medical University,