A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

Yiting TAO; Ge BAI; dan YU; Zhu ZHOU

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Yiting TAO, Ge BAI, dan YU, Zhu ZHOU. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

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Yiting TAO, Ge BAI, dan YU, Zhu ZHOU. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

Yiting TAO, Ge BAI, dan YU, Zhu ZHOU. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

Citation:

Yiting TAO, Ge BAI, dan YU, Zhu ZHOU. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods[J]. Journal of Kunming Medical University.

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A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods

Yiting TAO¹,
Ge BAI¹,
dan YU²,
Zhu ZHOU^{1
,
,}

1.
The 1st Department of Nephrology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650000
2.
Center of Clinical Pharmacy，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650000，China

Received Date: 2024-04-07

Abstract

Abstract

Objective To investigate the stability of the two kinds of rat models of chronic renal failure, aiming to provide a basis for establishing a feasible and stable animal model of chronic renal failure. Methods The rat model of chronic renal failure was established by unilateral nephrectomy + adenine gavage + high-phosphorus diet and high-phosphorus + adenine diet, respectively. Erythrocyte and hemoglobin levels, serum urea and creatinine levels were detected at the end of modeling and 6 weeks after modeling, and histopathological examinations of kidney and bone were performed. The tubulointerstitial fibrosis index （TBI） and the percentage of collagen fiber area in renal tissue were calculated. SPSS 26.0 software package was used to process the data. Results At the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 1（unilateral nephrectomy + adenine gavage + high-phosphorus） were significantly decreased, the serum urea and creatinine were significantly increased, TBI and the percentage of collagen fiber area in kidney tissue were slightly increased. HE staining revealed vacuolar degeneration of renal tubular epithelial cells, disappearance of the brush border, dilation of the renal tubular lumen, focal atrophy of the renal tubules, interstitial edema accompanied by a small amount of fibrosis, and infiltration of inflammatory cells. It was also observed that the arrangement of trabeculae was disordered and the number of osteoclasts increased. Masson staining showed a small amount of collagen fiber deposition in the renal interstitium. 6 weeks after the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 1 were slightly decreased, serum urea, creatinine, TBI and the percentage of collagen fiber area in renal tissue were slightly increased. HE staining revealed that the lumens of some renal tubules were slightly dilated, the renal interstitium was mildly edematous and accompanied by a small amount of fibrosis. No obvious lesions were observed in the femoral tissue. Masson staining indicated that there was a small amount of collagen fiber deposition in the renal interstitium. At the end of the modeling, the erythrocyte and hemoglobin in the model group 2 （high-phosphorus + adenine diet） were significantly decreased compared with the normal group, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased. HE staining revealed vacuolar degeneration of renal tubular epithelial cells, disappearance of the brush border, dilation of the renal tubular lumen, focal atrophy of the renal tubules, mild edema of the renal interstitium accompanied by obvious fibrosis, and a large number of inflammatory cell infiltrations. it can be observed that the arrangement of bone trabeculae was disordered, the number of local osteoclasts increased, and osteolytic changes were obvious. Masson staining indicated a large amount of collagen fiber deposition in the renal interstitium. 6 weeks after the end of modeling, compared with the normal group, the erythrocyte and hemoglobin in the model group 2 were significantly decreased, serum urea, creatinine, TBI and the percentage of collagen fiber area in kidney tissue were significantly increased. HE staining revealed the shedding of some renal tubular epithelial cells and the disappearance of the brush border, mild interstitial edema accompanied by obvious fibrosis, and a large number of inflammatory cell infiltrations. An increase in the number of osteoclasts and osteolytic changes were observed. Masson staining indicated a considerable amount of collagen fiber deposition in the renal interstitium. 6 weeks after the end of modeling, compared with the model group 1, the erythrocyte and hemoglobin in the model group 2 were significantly decreased, while the serum urea, creatinine, TBI, the collagen fiber area percentage of kidney tissue and femur lesions were significantly increased. HE staining revealed a large number of inflammatory cell infiltrations in the renal interstitium. It was observable that the arrangement of bone trabeculae was disordered, the number of local osteoclasts was increased, and the osteolytic changes were significant. Masson staining indicated obvious collagen fiber deposition in the renal interstitium. Conclusions A stable rat model of chronic renal failure can be established by high phosphorus + adenine diet.
- Rat,
- Chronic Renal Failure,
- Adenine,
- Stability

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References(17)

References

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