Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis

Aheng WANG; Zhen SONG; Guanghua YANG; Haiyan HE

doi:10.12259/j.issn.2095-610X.S20241107

Volume 45 Issue 11

Nov. 2024

Turn off MathJax

Article Contents

Abstract

References

Relative Articles

Article Navigation > Journal of Kunming Medical University > 2024 > 45(11): 46-51

Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis[J]. Journal of Kunming Medical University, 2024, 45(11): 46-51. doi: 10.12259/j.issn.2095-610X.S20241107

Citation:

Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis[J]. Journal of Kunming Medical University, 2024, 45(11): 46-51. doi: 10.12259/j.issn.2095-610X.S20241107

Citation:

PDF( 1501 KB)

Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis

doi: 10.12259/j.issn.2095-610X.S20241107

1.
Dept. of Head and Neck Surgery
2.
Dept. of Outpatient，Qingdao Central Hospital of Rehabilitation University，Qingdao Shandong 266000，China

Received Date: 2024-06-10
Available Online: 2024-10-23
Publish Date: 2024-11-25

Abstract

Abstract

Objective To explore the regulatory mechanism of TLR4/NF-κB/MUC5B in allergic rhinitis. Methods A mouse model of allergic rhinitis was established to observe the changes in pathological characteristics of the model and to detect the expression of TLR4 and MUC5B. By reducing the expression of TLR4 by intranasal administration, the histopathological changes of TLR4, the expression of MUC5B and the concentration of inflammatory factors were observed. An in vitro cell model was established to detect the expression of MUC5B as well as to observe the changes of NF-κB and phosphorylated NF-κB （p-NF-κB） after knockdown of TLR4 by cell transfection. Results （1） TLR4 and MUC5B were highly expressed in a mouse model of allergic rhinitis （P < 0.05）; （2） Lowering the expression of TLR4 could reduce the expression of MUC5B and inflammatory factors, as well as alleviated allergic rhinitis （P < 0.05）; （3） In vitro, knockdown of TLR4 could reduce the expression of p-NF-κB and MUC5B （P < 0.05）. Conclusion The regulatory mechanism of TLR4/NF-κB/ MUC5B in allergic rhinitis provides the new ideas for the treatment of allergic rhinitis.
- Allergic rhinitis,
- TLR4,
- MUC5B,
- NF-κB

FullText(HTML)

References(29)

References

[1]	Hoyte F C L,Nelson H S. Recent advances in allergic rhinitis[J]. F1000Res,2018,7(1):1.
[2]	Cohen B. Allergic rhinitis[J]. Pediatr Rev,2023,44(10):537-550. doi: 10.1542/pir.2022-005618
[3]	Skoner D P. Complications of allergic rhinitis[J]. J Allergy Clin Immunol,2000,105(6 Pt 2): S605-609.
[4]	Chirakalwasan N,Ruxrungtham K. The linkage of allergic rhinitis and obstructive sleep apnea[J]. Asian Pac J Allergy Immunol,2014,32(4):276-286.
[5]	Geng B, Dilley M, Anterasian C. Biologic therapies for allergic rhinitis and nasal polyposis[J]. Curr Allergy Asthma Rep,2021,21(6):36.
[6]	Park B S,Lee J O. Recognition of lipopolysaccharide pattern by TLR4 complexes[J]. Exp Mol Med,2013,45(12):e66. doi: 10.1038/emm.2013.97
[7]	Yang L,Lian Z,Zhang B,et al. Effect of ligustrazine nanoparticles on Th1/Th2 balance by TLR4/MyD88/NF-κB pathway in rats with postoperative peritoneal adhesion[J]. BMC Surg,2021,21(1):211. doi: 10.1186/s12893-021-01201-7
[8]	Wu J,Wu L,Zhang L,et al. Overexpression of miR-224-5p alleviates allergic rhinitis in mice via the TLR4/MyD88/NF-κB pathway[J]. Exp Anim,2021,70(4):440-449. doi: 10.1538/expanim.20-0195
[9]	Siddiqui Z A,Walker A,Pirwani M M,et al. Allergic rhinitis: Diagnosis and management[J]. Br J Hosp Med (Lond),2022,83(2):1-9.
[10]	Lim K H,Staudt L M. Toll-like receptor signaling[J]. Cold Spring Harb Perspect Biol,2013,5(1):a011247. doi: 10.1101/cshperspect.a011247
[11]	Veneziani I,Alicata C,Moretta L,et al. Human toll-like receptor 8 (TLR8) in NK cells: Implication for cancer immunotherapy[J]. Immunol Lett,2023,261(1):13-16.
[12]	Kang C,Li X,Liu P,et al. Tolerogenic dendritic cells and TLR4/IRAK4/NF-κB signaling pathway in allergic rhinitis[J]. Front Immunol,2023,14(1):1276512.
[13]	Chen S N,Tan Y,Xiao X C,et al. Deletion of TLR4 attenuates lipopolysaccharide-induced acute liver injury by inhibiting inflammation and apoptosis[J]. Acta Pharmacol Sin,2021,42(10):1610-1619. doi: 10.1038/s41401-020-00597-x
[14]	Tam J S Y,Coller J K,Hughes P A,et al. Toll-like receptor 4 (TLR4) antagonists as potential therapeutics for intestinal inflammation[J]. Indian J Gastroenterol,2021,40(1):5-21. doi: 10.1007/s12664-020-01114-y
[15]	Huang X,Guan W,Xiang B,et al. MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model[J]. Respir Res,2022,23(1):11. doi: 10.1186/s12931-021-01920-8
[16]	李增沛,李靖. 变应性鼻炎患者TNF-α、Tim-1及TLR4变化与病情程度的相关性研究[J]. 中国耳鼻咽喉颅底外科杂志,2023,29(6):50-53.
[17]	Dong J,Xu O,Wang J,et al. Luteolin ameliorates inflammation and Th1/Th2 imbalance via regulating the TLR4/NF-κB pathway in allergic rhinitis rats[J]. Immunopharmacol Immunotoxicol,2021,43(3):319-327. doi: 10.1080/08923973.2021.1905659
[18]	唐进渝,彭丽娟,钟春燕,等. 慢性鼻-鼻窦炎患者HIF-1α、FoxM1的表达及其与黏蛋白MUC5AC、MUC5B的相关性[J]. 西部医学,2023,35(11):1610-1614. doi: 10.3969/j.issn.1672-3511.2023.11.009
[19]	龙小博,甄宏韬,彭璐,等. 布地奈德对变应性鼻炎鼻黏膜黏蛋白MUC5AC和MUC5B表达的影响[J]. 华中科技大学学报(医学版),2007,36(5):648-651,704. doi: 10.3870/j.issn.1672-0741.2007.05.024
[20]	Płóciennikowska A,Hromada-Judycka A,Borzęcka K,et al. Co-operation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling[J]. Cell Mol Life Sci,2015,72(3):557-581. doi: 10.1007/s00018-014-1762-5
[21]	Tak P P,Firestein G S. NF-kappa B: A key role in inflammatory diseases[J]. J Clin Invest,2001,107(1):7-11. doi: 10.1172/JCI11830
[22]	Lawrence T. The nuclear factor NF-kappaB pathway in inflammation[J]. Cold Spring Harb Perspect Biol,2009,1(6):a001651.
[23]	Poma P. NF-κB and disease[J]. Int J Mol Sci,2020,21(23):81-91.
[24]	Bousquet J, Anto J M, Bachert C, et al. Allergic rhinitis[J]. Nat Rev Dis Primers,2020,6(1):95.
[25]	牛金明,程莉雅,吴美美. 柚皮素对过敏性鼻炎模型大鼠鼻黏膜组织TLR4/NF-κB/TNF-α信号通路的影响[J]. 中国实验诊断学,2023,27(4):483-488. doi: 10.3969/j.issn.1007-4287.2023.04.027
[26]	Zhu X,Sun Y,Yu Q,et al. Exosomal lncRNA GAS5 promotes M1 macrophage polarization in allergic rhinitis via restraining mTORC1/ULK1/ATG13-mediated autophagy and subsequently activating NF-кB signaling[J]. Int Immunopharmacol,2023,121(1):110-450.
[27]	Tian B, Ma X, Jiang R. Daphnetin mitigates ovalbumin-induced allergic rhinitis in mice by regulating Nrf2/HO-1 and TLR4/NF-kB signaling[J]. Am J Rhinol Allergy,2023,37(1):19-25.
[28]	Piao C H, Fan Y, Nguyen T V, et al. PM2.5 exposure regulates Th1/Th2/Th17 cytokine production through NF-κB signaling in combined allergic rhinitis and asthma syndrome[J]. Int Immunopharmacol,2023,119(1):110-254.
[29]	Zhao H, Yang J, Wang M, et al. Effect of IL-9 neutralising antibody on pyroptosis via SGK1/NF-κB/NLRP3/GSDMD in allergic rhinitis mice[J]. Biomed Pharmacother,2024,177(1):117-119.

Relative Articles(20)

Relative Articles

[1]	Zhuohui LIU, Shiyin QIN, Hexiang ZHAO, Fengfeng JIA, Biao RUAN, Ruiqing LONG. Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway. Journal of Kunming Medical University, 2024, 45(12): 19-27. doi: 10.12259/j.issn.2095-610X.S20241203
[2]	Yidan FU, Wenting CHEN, Xiaoyang SU, Yan ZHAO, Danfeng LAN, Qiuping YANG. Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells. Journal of Kunming Medical University, 2023, 44(12): 20-24. doi: 10.12259/j.issn.2095-610X.S20231203
[3]	Tingna ZHU, Yuanyuan CAO, Yuan ZHANG, Pengliang LIU, Yihang WANG, Yamei WU, Lihua LI, Yongna ZHAO, Shijun HONG. Effects of Different Doses of Gastrodin on TLR4/MyD88/NF-κB Inflammatory Signal Pathway in Hippocampus of Methamphetamine Dependent CPP Rats. Journal of Kunming Medical University, 2023, 44(2): 33-39. doi: 10.12259/j.issn.2095-610X.S20230226
[4]	Yuan ZHANG, Tingna ZHU, Yuanyuan CAO, Pengliang LIU, Yihang WANG, Yamei WU, Lihua LI, Yongna ZHAO, Shijun HONG. Effect of TLR4/MyD88/NF- κB Signal Pathway on the Hippocampus of Methamphetamine-dependent CPP Rats. Journal of Kunming Medical University, 2023, 44(2): 7-13. doi: 10.12259/j.issn.2095-610X.S20230225
[5]	Na PI, Qin HE, Yan ZHONG, Xuan JIN, Zhen-dong ZHU, Xuan ZHANG. Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis. Journal of Kunming Medical University, 2021, 42(4): 8-12. doi: 10.12259/j.issn.2095-610X.S20210402
[6]	Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117
[7]	Liu Jun , He Hong Bing , Ren Xiao Bin . . Journal of Kunming Medical University, 2019, 40(07): 7-14.
[8]	Shen Zhi Xiang , Zhu Li Xun , Xu Wei Hong . 有氧运动对2型糖尿病大鼠AGE-RAGE轴及NF-κB通路的影响. Journal of Kunming Medical University, 2018, 39(01): 16-19.
[9]	Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus. Journal of Kunming Medical University, 2018, 39(07): 5-9.
[10]	Tang Wei Wei , He Yong Wen . . Journal of Kunming Medical University, 2018, 39(10): 42-45.
[11]	Liao Ran Chao , Yu Yong Mei , Qiu Ji Wei , Xu Xue Mei . Predominant Species of House Dust Mites in Kunming. Journal of Kunming Medical University, 2017, 38(06): 56-59.
[12]	Zhang Jin Pei , Yang Yi , Jin Yan . Effect of Ma Xiong Shu Tong Fang on Migraine and Its Influence on Serum Levels of 5-HT, NF-NF-κB and Inflamatory Cytokines. Journal of Kunming Medical University, 2017, 38(08): 40-43.
[13]	Yang Rong , Yang Ying . . Journal of Kunming Medical University, 2017, 38(05): 44-49.
[14]	Chen Xin , Huang Pu , Jia Nan Nan , Chen Shao Chun , Ye Pin , Liu Cheng Xing , Li Guo Ping . Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft. Journal of Kunming Medical University, 2016, 37(12): 8-11.
[15]	Liu Xiao Chen . A Survey of Allergic Rhinitis among College Students in Kunming. Journal of Kunming Medical University,
[16]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[17]	Liao Ran Chao . . Journal of Kunming Medical University,
[18]	Yang Le . . Journal of Kunming Medical University,
[19]	Ma Shan . Effects of Liangxuehuoxue Drugs on Hyperoxia Induced Retinal Neovascular HIF-1 and NF-кB Expression in Mice. Journal of Kunming Medical University, 2012, 33(10): 1-1.
[20]	. Clinical Analysis of 32 Cases of Adults Allergic Purpura. Journal of Kunming Medical University,