冠脉搭桥术后静脉桥平滑肌细胞模型建立

张浒; 林玲; 杨爱玲; 苏蓉; 黄波

doi:10.12259/j.issn.2095-610X.S20241003

冠脉搭桥术后静脉桥平滑肌细胞模型建立

doi: 10.12259/j.issn.2095-610X.S20241003

张浒¹,
林玲¹,
杨爱玲¹,
苏蓉¹,
黄波^2, ,

1.
昆明医科大学第一附属医院老年心脏内科
2.
麻醉手术科，云南昆明　650032

基金项目: 云南省科技厅基础研究计划基金资助项目（202201AY070001-067）

详细信息

作者简介:
张浒（1978～），男，重庆人，医学博士，副教授，主要从事临床心血管疾病治疗和教学工作

通讯作者:
黄波，E-mail：1036496536@qq.com

中图分类号: R332
计量
- 文章访问数: 85
- HTML全文浏览量: 133
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2024-04-08
- 网络出版日期: 2024-10-21

Establishment of a Smooth Muscle Cell Model for Venous Grafts after Coronary Bypass Surgery

Hu ZHANG¹,
Ling LIN¹,
Ailing YANG¹,
Rong SU¹,
Bo HUANG^{2
, ,}

1.
Dept. of Geriatric Cardiology
2.
Operating Room，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

摘要

摘要: 目的探索冠脉搭桥静脉桥体外细胞模型的建立。方法体外培养大鼠静脉平滑肌细胞，正常对照组，5%张应力组，10%张应力组，15%张应力组4组，细胞拉伸装置不同拉伸幅度拉伸细胞，观察细胞形态变化，CCK-8检测观察细胞活性变化，Edu染色观察细胞增殖变化，划痕实验观察细胞迁移能力变化。结果与正常对照组相比较，5%张应力对细胞活度无显著影响（P > 0.05）；10%张应力明显促进细胞活力（P < 0.05）；而 15%张应力则明显抑制细胞活力（P < 0.01）。与正常对照组相比，5%张应力对细胞增殖无显著影响（P > 0.05）；10%张应力明显促进细胞增殖（P < 0.01）；15%张应力明显抑制细胞增殖（P < 0.05）。与正常组对比，5%张应力对细胞迁移能力无明显影响（P > 0.05）；10%张应力促进细胞迁移能力（P < 0.05）；15%张应力明显抑制细胞迁移能力（P < 0.05）。结论建立模型在频率1HZ，拉伸变形幅度10%参数设定下，血管平滑肌细胞与临床静脉桥再狭窄增生细胞生物行为表现一致。
- 静脉桥 /
- 模型 /
- 体外 /
- 血管平滑肌细胞 /
- 冠脉搭桥术
Abstract: Objective To explore the establishment of in vitro model of coronary artery bypass venous bridge in coronary artery bypass graft （CABG）. Methods Rat venous smooth muscle cells were cultured in vitro and divided into 4 groups. The cells were stretched by cell stretching device in order to simulate the mechanical environment of vein bridge. The changes of cell morphology were observed by CCK-8 detection, cell activity was observed by Edu staining, and cell migration ability was observed by scratch test. Results Compared to the normal control group, 5% tensile stress had no significant effect on cell viability （P > 0.05）; 10% tensile stress significantly enhanced cell vitality （P < 0.05）; while 15% tensile stress significantly suppressed cell vitality （P < 0.01）. In terms of cell proliferation, 5% tensile stress showed no significant effect compared to the normal control group （P > 0.05）; 10% tensile stress significantly promoted cell proliferation （P < 0.01）; and 15% tensile stress significantly inhibited cell proliferation （P < 0.05）. Compared to the normal group, 5% tensile stress had no obvious impact on cell migration capability （P > 0.05）; 10% tensile stress promoted cell migration ability （P < 0.05）; and 15% tensile stress significantly inhibited cell migration ability （P < 0.05）. Conclusion Under establishment the parameters of 1 Hz frequency and 10% stretch deformation, the biological behavior of vascular smooth muscle cells is consistent with that of clinical stenosis-restenosis cells.
- Venous bridge /
- Model /
- In vitro /
- Venous smooth muscle cells /
- Coronary artery bypass graft

HTML全文

图 1 各组VSMC形态观察（×25）

A：正常对照组；B：5%张应力组；C：10% 张应力组；D：15%张应力组。　　　　　　　　　　　　　　　

Figure 1. Morphology in each group of VSMC （×25）

下载: 全尺寸图片幻灯片

图 2 各组免疫荧光染色结果

Figure 2. Immunofluorescence staining in each group of VSMC

下载: 全尺寸图片幻灯片

图 3 各组细胞活力统计分析结果

Figure 3. Statistical analysis of CCK-8 in each group of VSMC

下载: 全尺寸图片幻灯片

图 4 各组EDU染色结果

与正常对照组比较，^**P < 0.05，^***P < 0.01。

Figure 4. EDU staining in each group of VSMC

下载: 全尺寸图片幻灯片

图 5 各组划痕愈率的统计分析结果

与正常对照组比较，ns P > 0.05，^**P < 0.05。

Figure 5. Statistical analysis of the proportion of wound healing in each group of VSMC

下载: 全尺寸图片幻灯片

参考文献(15)

[1]	Guida G A,Angelini G D. Pathophysiology and mechanisms of saphenous vein graft failure[J]. Braz J Cardiovasc Surg,2022,37(Spec 1): 32-37.
[2]	Kang L,Jia H,Huang B,et al. Identification of differently expressed mRNAs in atherosclerosis reveals CDK6 is regulated by circHIPK3/miR-637 axis and promotes cell growth in human vascular smooth muscle cells[J]. Front Genet,2021,12:596169-596181. doi: 10.3389/fgene.2021.596169
[3]	Duggan J P,Peters A S,Trachiotis G D,et al. Epidemiology of coronary artery disease[J]. Surg Clin North Am,2022,102(3):499-516. doi: 10.1016/j.suc.2022.01.007
[4]	胡盛寿. 中国心血管健康与疾病报告2021概要[J]. 中国循环杂志,2022,37(6):553-578. doi: 10.3969/j.issn.1000-3614.2022.06.001
[5]	中国生物医学工程学会体外循环分会,赵举,黑飞龙,等. 2021年中国心外科手术和体外循环数据白皮书[J]. 中国体外循环杂志,2022,20(4):196-199.
[6]	Caldonazo T,Kirov H,Riedel L L,et al. Comparing CABG and PCI across the globe based on current regional registry evidence[J]. Sci Rep,2022,12(1):22164-22174. doi: 10.1038/s41598-022-25853-4
[7]	Audisio K,Halbreiner M S,Chadow D,et al. Radial artery or saphenous vein for coronary artery bypass grafting[J]. Trends Cardiovasc Med,2022,32(8):479-484. doi: 10.1016/j.tcm.2021.09.007
[8]	Caliskan E,de Souza D R,Böning A,et al. Saphenous vein grafts in contemporary coronary artery bypass graft surgery[J]. Nat Rev Cardiol,2020,17(3):155-169. doi: 10.1038/s41569-019-0249-3
[9]	Sandner S,Redfors B,Angiolillo D J,et al. Association of dual antiplatelet therapy with ticagrelor with vein graft failure after coronary artery bypass graft surgery: A systematic review and meta-analysis[J]. JAMA,2022,328(6):554-562. doi: 10.1001/jama.2022.11966
[10]	Hosseini V,Mallone A,Nasrollahi F,et al. Healthy and diseased in vitro models of vascular systems[J]. Lab Chip,2021,21(4):641-659. doi: 10.1039/D0LC00464B
[11]	Abutaleb N O,Truskey G A. Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress[J]. STAR Protoc,2021,2(2):100394. doi: 10.1016/j.xpro.2021.100394
[12]	Jackson M L,Bond A R,George S J. Mechanobiology of the endothelium in vascular health and disease: In vitro shear stress models[J]. Cardiovasc Drugs Ther,2023,37(5):997-1010. doi: 10.1007/s10557-022-07385-1
[13]	Urschel K,Tauchi M,Achenbach S,et al. Investigation of wall shear stress in cardiovascular research and in clinical practice-from bench to bedside[J]. Int J Mol Sci,2021,22(11):5635-5654. doi: 10.3390/ijms22115635
[14]	贾丹,曹伟丽,黄立娟. microRNA调节血管平滑肌细胞在动脉粥样硬化中的作用[J]. 国际免疫学杂志,2023,46(3):341-345. doi: 10.3760/cma.j.issn.1673-4394.2023.03.019
[15]	张浒,钟佳冀,黄宏波,等. 灯盏花素对冠脉搭桥术移植静脉保护作用的实验[J]. 昆明医科大学学报,2019,40(11):26-29. doi: 10.3969/j.issn.1003-4706.2019.11.005

相关文章(20)

[1]	王惠, 曾文珺, 张海萍, 郭瑞威. 钙库操纵型钙通道Orai3分子对冠状动脉血管平滑肌细胞增殖的影响, 昆明医科大学学报. 2023, 44(4): 16-22. doi: 10.12259/j.issn.2095-610X.S20230423
[2]	张紫微, 郑甲林, 许晓宇, 王红. 二甲双胍通过AMPK/PPAR-γ通路诱导自噬抑制高糖对血管平滑肌细胞的增殖作用, 昆明医科大学学报. 2023, 44(10): 60-66. doi: 10.12259/j.issn.2095-610X.S20231026
[3]	张莉, 赵熙, 赵华祥, 熊宝, 徐洁, 汤广平, 喻卓, 陈鹏. 卫茅碱对人血管内皮细胞及血管平滑肌细胞增殖、迁移及细胞周期的影响, 昆明医科大学学报. 2023, 44(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20230723
[4]	张黎, 王清岑, 周罗慧, 杨娟, 王红. 木犀草素通过调控内质网应激抑制平滑肌细胞的迁移, 昆明医科大学学报. 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518
[5]	李玉晓, 贺铭, 王天蓉, 姚永刚, 王雨. Si-GATA-3对AR小鼠PBMCs中Th1/Th2细胞亚群功能的体外调节, 昆明医科大学学报. 2022, 43(8): 7-16. doi: 10.12259/j.issn.2095-610X.S20220802
[6]	张浒, 彭安睿, 钟佳冀, 黄波, 白鹏. 灯盏乙素在抑制冠脉搭桥术后静脉桥再狭窄中的应用, 昆明医科大学学报. 2022, 43(1): 59-62. doi: 10.12259/j.issn.2095-610X.S20220136
[7]	宿玮洁, 张美沙, 李佳凝, 刘颖, 徐丁洁, 常宏. Ang Ⅱ诱导大鼠成肌细胞萎缩模型的构建, 昆明医科大学学报. 2021, 42(10): 8-13. doi: 10.12259/j.issn.2095-610X.S20211026
[8]	张浒, 钟佳冀, 黄宏波, 罗雪婷, 黄波, 陶杰. 灯盏花素对冠脉搭桥术移植静脉保护作用的实验, 昆明医科大学学报. 2019, 40(11): 26-29.
[9]	李具宝, 张锡华, 张晓刚, 杨学锋, 李延红, 刘太运, 董建华. 利用CT图像和ADINA软件建立新型正常中国男性腰椎运动节段三维有限元模型, 昆明医科大学学报. 2018, 39(12): 38-42.
[10]	张柽. 猪体外并行循环模型的建立及管理, 昆明医科大学学报. 2015, 36(07): -1.
[11]	曹宇. 环磷酰胺和白消安构建Wistar大鼠非梗阻性无精子症模型的比较, 昆明医科大学学报. 2015, 36(05): -.
[12]	陈刚. 猪胆道缺损修补实验动物模型的建立, 昆明医科大学学报. 2014, 35(12): -1.
[13]	张敏. 外源性碱性成纤维细胞生长因子诱导下自体静脉桥接修复面神经缺损, 昆明医科大学学报. 2013, 34(07): -.
[14]	赵瑜敏. 牙龈卟啉单胞菌对兔血管平滑肌细胞增殖和迁移的影响, 昆明医科大学学报. 2013, 34(03): -.
[15]	苏晓三. 两种外周血单个核细胞采集方法体外诱导CIK细胞的比较, 昆明医科大学学报. 2013, 34(06): -.
[16]	张敏. 翻转静脉桥接加碱性成纤维细胞生长因子修复面神经缺损的实验研究, 昆明医科大学学报. 2012, 33(05): -.
[17]	. 改进背驮式肝移植动物模型的建立和评价, 昆明医科大学学报. 2011, 32(02): -.
[18]	朱洪. 改进背驮式肝移植动物模型的建立和评价, 昆明医科大学学报. 2011, 32(02): -.
[19]	. 心血管外科进展, 昆明医科大学学报. 2011, 32(03): -.
[20]	丁鹏. 新生大鼠海马神经干细胞表达趋化因子受体CCR2的体外研究, 昆明医科大学学报. 2008, 29(01): -.