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袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响

夏军 李伟思 张文杰 戚宗泽

夏军, 李伟思, 张文杰, 戚宗泽. 袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响[J]. 昆明医科大学学报.
引用本文: 夏军, 李伟思, 张文杰, 戚宗泽. 袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响[J]. 昆明医科大学学报.
Jun XIA, Weisi LI, Wenjie ZHANG, Zongze QI. Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice[J]. Journal of Kunming Medical University.
Citation: Jun XIA, Weisi LI, Wenjie ZHANG, Zongze QI. Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice[J]. Journal of Kunming Medical University.

袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响

基金项目: 昆明市卫生科技基金资助项目(202104010010);昆明医科大学研究生教育创新基金资助项目(2022S288)
详细信息
    作者简介:

    夏军(1994~),男,云南曲靖人,医学硕士,住院医师,主要从事肝胆胰外科基础与临床研究工作

    通讯作者:

    戚宗泽,E-mail:648655143@qq.com

  • 中图分类号: R-332

Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice

  • 摘要:   目的  探讨肥胖体质小鼠在接受袖状胃切除术(sleeve sastrectomy,SG)前后肝脏自噬因子活性的变化。  方法  6~8周龄的雄性肥胖小鼠随机分成假手术组(Sham组,n=6)和袖状胃切除手术组(SG组,n=6),在术后1个月内记录2组小鼠每日体重和日摄食量,并在术后15 d和30 d时测定小鼠尾尖末端空腹血糖值,进行口服葡萄糖耐量试验以评估葡萄1糖的吸收情况;采用RT-qPCR检测术后15 d和30 d自噬因子以及葡萄糖转运蛋白的mRNA表达变化情况。  结果  与术前相比,Sham组术后15 d和30 d的体重、日摄食量、空腹血糖值均无统计学意义(P > 0.05),而SG组术后15 d和30 d体重、日摄食量、空腹血糖值均降低且差异具有统计学意义(P < 0.05);与Sham组相比,SG组15 d和30 d的体重、日摄食量、空腹血糖均减少且具有统计学意义(P < 0.05),SG组小鼠自噬因子P62增高,而Beclin-1、LC3I/LC3II及SGLT1的mRNA表达在术后15 d和30 d均降低且差异具有统计学意义(P < 0.05)。  结论  SG能够改善肥胖而降低炎症介质与脂肪因子进一步调节小鼠肝脏自噬活性。
  • 图  1  小鼠体重和日摄食量的变化(χ2±SEM,n=6)

    A:Sham组与SG组术后30 d内小鼠体重的变化;B:Sham组与SG组术后30 d内小鼠日摄食量的变化。与Sham组比较,*P<0.05,**0.05<P<0.01,***P<0.001。

    Figure  1.  Changes in body mass and daily food intake of mouse(χ2±SEM,n=6)

    图  2  小鼠空腹血糖值、口服葡萄糖耐量试验血糖变化及曲线下面积(χ2±SEM,n=6)

    A:Sham组与SG组术前、术后15 d和30 d小鼠的空腹血糖值;B~E:Sham组与SG组小鼠术后15 d、30 d进行口服葡萄糖耐量试验血糖变化水平及血糖变化曲线下面积。与Sham组比较,ns P>0.05,*P<0.05,**0.05<P<0.01,***P<0.001。

    Figure  2.  Fasting blood glucose levels,oral glucose tolerance test blood glucose changes,and area under the curve in mouse(χ2±SEM,n=6)

    图  3  术后肝脏自噬因子(P62、Beclin-1、LC3I、LC3II)及肠道SGLT1的mRNA表达变化(χ2±SEM,n=6)

    A:Sham组与SG组术后15 d、30 d小肠的SGLT1 mRNA表达丰度;B:Sham组与SG组术后15 d、30 d肝脏的P62 mRNA表达丰度;C:Sham组与SG组术后15 d、30 d肝脏的Beclin-1 mRNA表达丰度;D~E:Sham组与SG组术后15 d、30 d肝脏的LC3I、LC3II mRNA表达丰度。与Sham组比较,ns P>0.05,*P<0.05,**0.05<P<0.01,***P<0.001。

    Figure  3.  Changes in mRNA expression of liver autophagy factors (P62,Beclin-1,LC3I,LC3II) and intestinal SGLT1 after surgery(χ2±SEM,n=6)

    表  1  引物序列

    Table  1.   Primer sequence

    基因正向引物反向引物
    SGLT1 F:5'-CCAAGCCCATCCCAGACGTACACCC-3' R:5'-CTTCCTTAGTCATCTTCGGTCCTT-3'
    P62 F:5'-ATGTGGAACATGGAGGGAAGA-3' R:5'-GGAGTTCACCTGTAGATGGGT-3'
    Beclin1 F:5'-CCAGATGCGTTATGCCCAGAC-3' R:5'-CATTCCATTCCACGGGAACAC-3'
    LC3I F:5'-TATGGCACCAGTACGACG-3' R:5'-GCATTGTCAGTCACC-3'
    LC3II F:5'-TAGGCGATATAGCCGAACG-3' R:5'-CGGATAATGGATCAT-3'
    GAPDH F:5'-AGGTCGGTGTGAACGGATTTG-3' R:5'-TGTAGACCATGTAGTTGAGGTCA-3'
    下载: 导出CSV
  • [1] Salah R O, Ghandour R, Husseini A. Prevalence of overweight, obesity, and associated factors among adolescents in the occupied Palestinian territory: A cross-sectional study[J]. Lancet, 2021, 398(Suppl 1): S46.
    [2] Ruze R, Liu T, Zou X, et al. Obesity and type 2 diabetes mellitus: connections in epidemiology, pathogenesis, and treatments[J]. Front Endocrinol (Lausanne), 2023, 14: 1161521.
    [3] Runkel N,Brydniak R. Surgical treatment of metabolic syndromeJ7[J]. Visc Med,2016,32(5):352-356. doi: 10.1159/000449110
    [4] Aminian A,Wilson R,Zajichek A,et al. Cardiovascular outcomes in patients with type 2 diabetes and obesity: Comparison of gastric bypass,sleeve gastrectomy,and usual care[J]. Diabetes Care,2011,44(11):2552-2563.
    [5] Schauer P R, Bhatt D L, Kirwan J P, et al.Bariatric surgeryversus intensive medical therapy for diabetes-3-year out-comes[J]. The New England Journal of Medicine. 2015, 370(21):2002-2013.
    [6] Lippai M, Szatmari Z. Autophagy-from molecular mechanisms to clinical relevance. Cell Biol Toxicol, 2017, 33(2): 145-168 .
    [7] Debnath J, Gammoh N, Ryan KM. Autophagy and autophagy-related pathways in cancer[J]. Nat Rev Mol Cell Biol. 2023, 24(8):560-575.
    [8] Zhang Y, Higgins CB, Van Tine BA, et al. Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism[J]. Cell Rep Med. 2022 Jan 18;3(1): 100498.
    [9] Barzin M,Khalaj A,Motamedi MA,et al. Safety and effectiveness of sleeve gastrectomy versus gastric bypass: one-year results of Tehran Obesity Treatment Study (TOTS)[J]. Gastroenterol Hepatol Bed Bench.,2016,9(Suppl1):S62-S69.
    [10] 史晓萍,宗阿南,陶钧,等.《关于善待实验动物的指导性意见》的研究[J].中国医科大学学报,2007,(04):493.
    [11] Yardimci E,Bozkurt S,Cengiz MB,et al. Comparison of WeightLoss,Ghrelin,and LeptinHormones After Ligation of Left Gastric Artery and Sleeve Gastrectomy in a Rat Model[J]. Medicalscience Monitor,2017,23:1442-1447.
    [12] Xia Jun, He Qian, He Ming, et al. Residual Gastric Dilatation Interferes with Metabolic Improvements Following Sleeve Gastrectomy by Upregulating the Expression of Sodium-Glucose Cotransporter-1[J]. Obesity surgery, 2019, 29(10): 3324-3333.
    [13] Evers SS, Lewis AG, Tong C, et al. The Unconventional Role for Gastric Volume in the Response to Bariatric Surgery for Both Weight Loss and Glucose Lowering[J]. Ann Surg. 2020 Jun;271(6): 1102-1109.
    [14] Santoleri D, Titchenell PM. Resolving the Paradox of Hepatic Insulin Resistance[J]. Cell Mol Gastroenterol Hepatol. 2019;7(2): 447-456.
    [15] Ye R, Gordillo R, Shao M, et al. Intracellular lipid metabolism impairs β cell compensation during diet-induced obesity[J]. J Clin Invest. 2018, 128(3): 1178-1189.
    [16] Song H, Zhang X, Wang J, et al. The regulatory role of adipocyte mitochondrial homeostasis in metabolism-related diseases[J]. Front Physiol, 2023, 14: 1261204.
    [17] 徐春阳,李文兰,杨秀颖,等. 脂肪代谢障碍与抗炎治疗策略[J]. 中药药理与临床,2018,34(5):168-172.
    [18] Song B Q,Chi Y,Li X,et al. Inhibition of notch signaling promotes the adipogenic differentiation of mesenchymal stem cells through autophagy activation and PTEN-PI3K /AKT /mTOR pathway[J]. Cell Physiol Biochem,2015,36(5):1991-2002. doi: 10.1159/000430167
    [19] Ma T,Li J,Xu Y,et al. Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming[J]. Nat Cell Biol,2015,17(11):1379-1387. doi: 10.1038/ncb3256
    [20] Tao Z,Liu L,Zheng L D,et al. Autophagy in Adipocyte Differentiation[J]. Methods in Molecular Biology,2019,1854:45-53.
    [21] Matsuzawa-Ishimoto Y, Hwang S, Cadwell K. Autophagy and Inflammation [J]. Annu Rev Immunol. 2018 Apr 26;36:73-101.
    [22] 张一文,喻松仁,姚琦,等. 细胞自噬调控肥胖脂肪组织炎症状态的研究进展[J]. 江西中医药,2020,51(8):77-80.
    [23] Weidberg H, Shvets E, ShpiLKAka T, et al. LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis [J/OL]. EMBO J, 2010, 29 (11): 1792-1802 .
    [24] Wang K,Chen Y,Zhang P,et al. Protective features of autophagy in pulmonary infection and inflammatory diseases[J]. Cells,2019,8(2):123. doi: 10.3390/cells8020123
    [25] Marasco M R,Linnemann A K. Beta-cell autophagy in diabetes pathogenesis[J]. Endocrinology,2018,159(5):2127. doi: 10.1210/en.2017-03273
    [26] 杨丽娟,洪逸莲,林怡,等. 人参皂苷 Rb2 通过抑制自噬促进肥胖小鼠白色脂肪棕色化[J]. 中华内分泌代谢杂志,2020,36(12):1055-1061.
    [27] Barlow A D,Thomas D C. Autophagy in diabetes: beta cell dysfunction,insulin resistance,and complications[J]. DNA Cell Biol,2015,34(4):252-260. doi: 10.1089/dna.2014.2755
    [28] KIM K H,LEE M S. Autophagy-a key player in cellular and body metabolism[J]. Nat Rev Endocrinol,2015,10(6):322-337.
    [29] Gonzalez C D,Lee M S,Marchetti P,et al. The emerging role of autophagy in the pathophysiology of diabetes mellitus[J]. Autophagy,2011,7(1):2-11. doi: 10.4161/auto.7.1.13044
    [30] Kosacka J, Kern M, Klöting N, et al. Autophagy in adipose tissue of patients with obesity and type 2 diabetes [J]. Molecular and Cellular Endocrinology, 2015, 409(Issue C): 21-32.
    [31] 李璇, 向小姣, 刘露路, 等. 自噬相关基因在肥胖患者及肥胖小鼠脂肪组织中的研究[J]. 重庆医科大学学报, 2016, 41(11): 1159-1162.
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  • 收稿日期:  2023-11-03
  • 网络出版日期:  2024-02-27

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