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益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用

梁国晶 冀琨 张恺纯 张玉芳 安晶 张钰鸽 文娟 任海燕

梁国晶, 冀琨, 张恺纯, 张玉芳, 安晶, 张钰鸽, 文娟, 任海燕. 益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用[J]. 昆明医科大学学报, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506
引用本文: 梁国晶, 冀琨, 张恺纯, 张玉芳, 安晶, 张钰鸽, 文娟, 任海燕. 益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用[J]. 昆明医科大学学报, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506
Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506
Citation: Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用

doi: 10.12259/j.issn.2095-610X.S20240506
基金项目: 新疆维吾尔自治区自然科学基金资助项目(2023D01C38)
详细信息
    作者简介:

    梁国晶(1999~),女,甘肃古浪人,在读硕士研究生,主要从事神经病理生理学研究工作

    通讯作者:

    任海燕,E-mail:424732833@qq.com

  • 中图分类号: R741.02;R363

The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury

  • 摘要:   目的  探讨益生菌干预对脑缺血再灌注损伤大鼠脑组织Aβ表达的影响及神经元的保护作用。  方法  实验动物为雄性Sprague-Dawley大鼠,随机分为4组,各组n = 12:假手术组、模型组、益生菌组、依达拉奉组。假手术组仅分离颈总动脉,其余各组使用线拴法建立脑缺血再灌注模型。脑损伤程度使用神经行为学评分评估;大鼠脑组织梗死面积使用TTC染色观察;大鼠大脑海马CA1区与皮层区神经元病理形态学改变特征使用HE染色观察;Aβ蛋白的表达使用免疫组化实验检测。  结果  模型组相较假手术组,神经行为学评分升高(P < 0.05),脑梗死面积增加(P < 0.05),海马CA1区与皮层区神经元出现损伤,Aβ蛋白表达显著上调(P < 0.001,P < 0.05);益生菌组、依达拉奉组与模型组相比,神经行为学评分降低(P < 0.05),脑梗死面积减小(P < 0.05),海马CA1区与皮层区神经元损伤减轻,Aβ蛋白表达水平在两组中均显著下调(P < 0.001,P < 0.05);依达拉奉组改善更为明显。  结论  益生菌干预可下调Aβ蛋白的表达,减轻脑缺血再灌注大鼠脑组织损伤、发挥神经元保护作用。
  • 图  1  各组大鼠脑组织梗死灶

    A:假手术组;B:模型组;C:益生菌组;D:依达拉奉组。

    Figure  1.  Cerebral infarction focus of rats in each group

    图  2  各组大鼠大脑海马CA1区与皮层区神经元病理形态学改变(×400)

    A~D:大鼠海马CA1区神经元病理形态学改变;E~H:大鼠皮层区神经元病理形态学改变。

    Figure  2.  Pathological and morphological changes of neurons in the hippocampus CA1 and cortical regions of rats in each group (×400)

    图  3  各组大鼠大脑海马CA1区与皮层区Aβ蛋白的表达

    A~D:大鼠海马CA1区神经元Aβ蛋白表达情况;E~H:大鼠皮层区神经元Aβ蛋白表达情况。

    Figure  3.  Expression of Aβ protein in the hippocampal CA1 and cortical regions of rats in each group

    图  4  各组大鼠大脑海马CA1区与皮层区Aβ蛋白的表达比较

    A:大鼠大脑海马CA1区Aβ蛋白的表达统计图;B:大鼠大脑皮层区Aβ蛋白的表达统计图与模型组比较,**P < 0.05,****P < 0.001。

    Figure  4.  Comparison of protein expression in the hippocampal CA1 and cortical regions of rats in each groups

    表  1  Zea Longa 5 级评分法评分标准

    Table  1.   Zea longa grade 5 scoring method scoring standard

    评分表现
    0无神经损害症状
    1梗死侧对侧前肢和前爪不能完全伸展
    2不能正常行走,行走时向梗死侧旋转
    3行走时向梗死对侧倾倒
    4无法行走和意识障碍
    下载: 导出CSV

    表  2  各组大鼠神经行为学评分[($ \bar x \pm s $),分]

    Table  2.   Neurobehavioral scores of rats in each group[ ($ \bar x \pm s $),points]

    分组 n 神经行为学评分
    假手术组 12 0±0*
    模型组 12 3.51±0.41
    益生菌组 12 2.53±0.41*
    依达拉奉组 12 2.27±0.20*
      与模型组比较,*P < 0.05。
    下载: 导出CSV

    表  3  各组大鼠脑组织梗死灶体积的比较[($ \bar x \pm s$)%]

    Table  3.   Comparison of cerebral infarction focus volume of rats in each group [($ \bar x \pm s$)%]

    分组 n 脑梗死体积百分比
    假手术组 3 0±0*
    模型组 3 21.56±2.24
    益生菌组 3 9.48±1.43*
    依达拉奉组 3 6.99±1.32*
      与模型组比较,*P < 0.05。
    下载: 导出CSV
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  • 收稿日期:  2024-01-05
  • 网络出版日期:  2024-04-30
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