恶性胸水培养肿瘤细胞失败原因分析
The Cause Analysis on the Failure of Tumor Cells Culture from Malignant Hydrothorax
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摘要: 目的 目前在肿瘤化疗体外药敏及生物免疫治疗中很重要的一个步骤是获取原代肿瘤细胞,通过对培养失败的原因分析,揭示肺部肿瘤细胞原代培养中应注意的问题,以便今后选择正确的培养方法,避免再走弯路.方法 无菌条件下取恶性胸水沉渣接种于RPMI Medium 1640,观察细胞生长情况.结果 15例标本培养观察约10 d能见到大量成纤维细胞生长,8例标本曾见到肿瘤细胞生长,但继续培养后肿瘤细胞死亡.8例未见到肿瘤细胞生长.结论 胸水采集量不足,沉淀后细胞数量少是失败的最主要因素;未及时送培养;未行冷藏送检;未及时清除成纤维细胞均为失败的重要原因.Abstract: Objective Obtaining primary cells is a very important procedure in sensitivity of chemotherapeutics in vitro and immunotherapy.This article is to reveal need advertent thing in culturing primary tumor cells by analyzing the culture failure cause,so as to select right culture method and avoid failure in the future.Method The cells of sediment from malignant hydrothorax were vaccinated at RPMI Medium 1640 in aseptic condition to observe cell growing.Results The many fibroblasts grew in fifteen collection samp...
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Key words:
- Malignant hydrothorax /
- Cell culture /
- Cause /
- Analysis /
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