UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布

卢发焕; 李继印; 李树华; 赵文嵩; 王蕊; 解润芳; 杨凯润

UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布

1. 腾冲市公安局
2. 昆明医科大学法医学院
3. 云南省公安厅刑侦总队

基金项目:

基金：云南省科技计划基金资助项目 (2013FB118); 昆明医科大学科技创新团队建设基金资助项目 (CXTD201604); 云南省教育厅科学研究基金资助项目 (2017z DX161);

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出版历程
- 收稿日期: 2018-03-13

Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method

Funds:

基金：云南省科技计划基金资助项目 (2013FB118); 昆明医科大学科技创新团队建设基金资助项目 (CXTD201604); 云南省教育厅科学研究基金资助项目 (2017z DX161);

摘要

摘要: 目的建立灌胃给药滇乌碱的大鼠中毒模型, 用高效液相色谱-串联质谱 (UPLC-MS/MS) 法测定大鼠血液及各组织中滇乌碱的含量, 研究滇乌碱在大鼠体内的分布情况.方法将SD大鼠随机分为3组, 分别按2.2 mg/kg, 1.1 mg/kg和0.7 mg/kg剂量灌胃高、中、低剂量组大鼠, 给药2 h后处死, 迅速采取大鼠的心血、胃、小肠、肝脏、胰腺、肾脏、肺脏、脾脏、心脏、膀胱、睾丸和全脑, 液-液萃取法处理后, 用UPLC-MS/MS法测定各生物检材中滇乌碱的含量.结果滇乌碱大鼠中毒模型给药剂量为1.1 mg/kg, 滇乌碱在大鼠体内分布由高到低的顺序为胃、小肠、肝脏、胰腺、肾脏、肺脏、脾脏、心脏、膀胱、睾丸、心血和大脑.结论滇乌碱在大鼠体内分布广泛, 尤以胃、小肠和肝脏中含量最高, 该结论为黄草乌中毒的检验材料选取提供依据.
- 黄草乌 /
- 滇乌碱 /
- UPLC-MS/MS /
- 大鼠 /
- 体内分布
Abstract: Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Me thod The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2 mg/kg, 1.1 mg/kg, 0.7 mg/kg yunaconitine, respectively.. The rats were killed 2 h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Re s ult A rat model of the yunaconitine poisoning was made with a single dose of 1.1 mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclus ion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
- Aconitum vilmorinianum Kom /
- Yunaconitine /
- UPLC-MS/MS /
- Rat /
- Tissue distribution

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