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维生素D受体基因Bg1I、Cdx-2位点多态性与桥本氏甲状腺炎的相关性

阮小荟 向茜 王玉明 周治含 张弦 郭燕 杨晓瑞

阮小荟, 向茜, 王玉明, 周治含, 张弦, 郭燕, 杨晓瑞. 维生素D受体基因Bg1I、Cdx-2位点多态性与桥本氏甲状腺炎的相关性[J]. 昆明医科大学学报, 2021, 42(8): 132-139. doi: 10.12259/j.issn.2095-610X.S20210824
引用本文: 阮小荟, 向茜, 王玉明, 周治含, 张弦, 郭燕, 杨晓瑞. 维生素D受体基因Bg1I、Cdx-2位点多态性与桥本氏甲状腺炎的相关性[J]. 昆明医科大学学报, 2021, 42(8): 132-139. doi: 10.12259/j.issn.2095-610X.S20210824
Xiao-hui RUAN, Qian XIANG, Yu-ming WANG, Zhi-han ZHOU, Xian ZHANG, Yan GUO, Xiao-rui YANG. The Correlation between Vitamin D Receptor Gene Bg1I and Cdx-2 Polymorphism and Hashimoto’ s Thyroiditis[J]. Journal of Kunming Medical University, 2021, 42(8): 132-139. doi: 10.12259/j.issn.2095-610X.S20210824
Citation: Xiao-hui RUAN, Qian XIANG, Yu-ming WANG, Zhi-han ZHOU, Xian ZHANG, Yan GUO, Xiao-rui YANG. The Correlation between Vitamin D Receptor Gene Bg1I and Cdx-2 Polymorphism and Hashimoto’ s Thyroiditis[J]. Journal of Kunming Medical University, 2021, 42(8): 132-139. doi: 10.12259/j.issn.2095-610X.S20210824

维生素D受体基因Bg1I、Cdx-2位点多态性与桥本氏甲状腺炎的相关性

doi: 10.12259/j.issn.2095-610X.S20210824
基金项目: 云南省教育厅科学研究基金资助项目(2018JS255);云南省卫生健康委医学学科带头人培养计划基金资助项目(D-2018034)
详细信息
    作者简介:

    阮小荟(1990~),女,云南金平人,医学学士,住院医师,主要从事内分泌代谢性疾病的临床诊治及医学教育工作

    通讯作者:

    向茜,E-mail:qian921004@sina.com

  • 中图分类号: R581.4

The Correlation between Vitamin D Receptor Gene Bg1I and Cdx-2 Polymorphism and Hashimoto’ s Thyroiditis

  • 摘要:   目的  探讨VDR基因Bg1I、Cdx-2多态性与桥本氏甲状腺炎(hashimoto’ s thyroiditis,HT)的相关性。  方法  应用TaqMan 荧光探针技术,对178例HT组,其中甲功正常(H0)组56例,亚临床甲减(H1)组80例,甲减(H2)组42例和64例健康对照者(NT)组的VDR基因Bg1I、Cdx-2位点SNP进行检测,比较和分析各组间基因型频率、等位基因频率以及相关临床资料。  结果  (1)Bg1I位点GG、GT、TT基因型分布频率分别为0.500,0.405和0.095 ,等位基因G、T分布频率分别为0.7025和0.2975;Cdx-2位点GG、GA、AA基因型分布频率分别为0.2934,0.5289 和0.1777,等位基因G及A分布频率分别为0.5579及0.4421;(2)NT及HT组间各等位基因频率及基因型分布,差异无统计学意义(P > 0.05);(3)Logistic回归分析表明25(OH)D缺乏可能是HT发生的独立危险因素(OR = 1.573,P = 0.046);(4)男性(OR = 0.158,P < 0.001)及Cdx-2位点G等位基因(OR = 0.301,P = 0.035)可能是HT患者发生甲减的保护因素,高滴度TPO-Ab(OR = 1.639,P = 0.045)及TG-Ab(OR = 1.779,P = 0.007)可能是其危险因素。  结论  (1)VDR Bg1I、Cdx-2位点存在单核苷酸多态性。(2)Cdx-2位点G等位基因可能是HT发生甲减的保护因素;(3)Bg1I位点SNP与HT的发生及进展无关。
  • 图  1  VDR基因 Bg1I位点SNP分型结果

    Figure  1.  Typing of VDR gene Bg1I SNP

    图  2  VDR基因Cdx-2位点SNP分型结果

    Figure  2.  Typing of VDR gene Cdx-2 SNP

    表  1  组间临床资料的比较[($\bar x \pm s $)或M(P25,P75)](1)

    Table  1.   Comparison of clinical data among different groups [($\bar x \pm s $)or M(P25,P75)]

    组别n性别(男/女)年龄/岁25(OH)D/(ng/mL)TT3/(ng/dL)TT4/(μg/dL)FT3/(pg/mL)
    NT组64 13/51 39.66 ± 12.58 20.17 ± 6.95 102.20(90.33,116.00) 8.11(6.26,9.60) 2.78(2.57,3.08)
    HT组178 24/154 45.02 ± 13.02* 17.49 ± 7.28* 101.50(80.60,122.00) 7.37(5.58,9.08) 2.77(2.40,3.02)
    HT0组56 2/54 43.43 ± 12.71 18.68 ± 7.77 104.50(89.20,122.75) 8.31(6.97,10.31) 2.81(2.60,3.04)
    HT1组80 9/71 45.48 ± 12.83* 17.15 ± .34* 103.17(86.83,125.75) 7.61(6.45,9.15) 2.85(2.52,3.20)
    HT2组42 13/29 46.29 ± 13.90 16.55 ± 6.38* 81.40(61.23,100.20)*△☆ 3.67(0.93,5.98)*△☆ 2.05(1.00,2.53)*△☆
      与NT组相比,*P < 0.05;与HT0组相比,P < 0.05;与HT1组相比,P < 0.05。
    下载: 导出CSV

    表  1  组间临床资料的比较($\bar x \pm s $)或M(P25,P75)(2)

    Table  1.   Comparison of clinical data among different groups ($\bar x \pm s$) or M(P25,P75)

    组别nFT4
    /(ng/dL)
    TSH
    /(mIU/L)
    TPO-Ab
    /(IU/mL)
    TG-Ab
    /(IU/mL)
    NT组 64 1.16(1.08,1.26) 2.01(1.49,2.70) 3.31(1.90,7.27) 13.33(10.54,25.75)
    HT组 178 1.01(0.79,1.23)* 5.52(2.93,16.78)* 334.75(36.40,16.37)* 285.55(88.29,936.80)*
    HT0组 56 1.17(0.98,1.36) 1.89(1.57,2.72) 47.52(27.49,426.00)* 130.45(24.21,362.13)*
    HT1组 80 1.09(0.91,1.26)*△ 5.98(4.61,9.78)*△ 360.67(74.13,1175.25)*△ 313.55(121.55,821.20)*△
    HT2组 42 0.55(0.29,0.71)*△ 62.95(26.81,100.00)*△☆ 1666.00(92.48,2001.43)*△☆ 858.65(254.58,3449.21)*△
      与NT组相比,*P < 0.05;与HT0组相比P < 0.05;与HT1组相比P < 0.05。
    下载: 导出CSV

    表  2  2组25(OH)D状况比较[n(%)]

    Table  2.   Comparison of 25(OH)D level between two groups [n(%)]

    组别n充足不足缺乏
    NT组 64 4(6.25) 28(43.75) 32(50.00)
    HT组 178 12(6.74) 44(24.72) 122(68.54)
    Z 2.799
    P 0.005
    下载: 导出CSV

    表  3  各组间VDR基因Bg1I位点SNP频率[n(%)]

    Table  3.   VDR gene Bg1I among each group [n(%)]

    组别
    n
    基因型等位基因
    GGGTTTGT
    Totle 242 121(50.00) 98(40.50) 23(9.50) 340(70.25) 144(29.75)
    NT 64 27(42.19) 32(50.00) 5(7.81) 86(67.19) 42(32.81)
    HT 178 94(52.81) 66(37.08) 18(10.11) 254(71.35) 102(28.65)
    HT0 56 34(60.71) 16(28.58) 6(10.71) 84(75.00) 28(25.00)
    HT1 80 37(46.25) 37(46.25) 6(7.50) 111(69.38) 49(0.62)
    HT2 42 23(54.76) 13(30.95) 6(14.29) 59(70.24) 25(29.76)
    下载: 导出CSV

    表  4  各组间VDR基因Cdx-2位点SNP频率[n(%)]

    Table  4.   VDR gene Cdx-2 among each group [n(%)]

    组别n基因型等位基因
    GGGAAAGA
    Totle 242 71(29.34) 128(52.89) 43(17.77) 270(55.79) 214(44.21)
    NT 64 18(28.12) 38(59.38) 8(12.50) 74(57.81) 54(42.19)
    HT 178 53(29.78) 90(50.56) 35(19.66) 196(55.06) 160(44.94)
    HT0 56 20(35.71) 25(44.65) 11(19.64) 65(58.04) 47(41.96)
    HT1 80 24(30.00) 38(47.50) 18(22.50) 86(53.75) 74(46.25)
    HT2 42 9(21.43) 27(64.29) 6(14.28) 45(53.57) 39(46.43)
    下载: 导出CSV

    表  5  HT发生危险因素的Logistic回归分析

    Table  5.   The logistic regression analysis of HT risk factors

    自变量BS.E.WalsdfPOR95%CI
    下限上限
    常量 0.328 0.371 0.786 1 0.375 1.389
    25(OH)D缺乏 0.453 0.227 3.977 1 0.046 1.573 1.008 2.456
    下载: 导出CSV

    表  6  HT甲减发生危险因素的Logistic回归分析

    Table  6.   The logistic regression analysis of risk factors for Hypothyroidism in HT group

    自变量
    B
    S.E.
    Wals
    df
    P
    OR
    95%CI
    下限上限
    常量 −0.083 1.529 0.003 0.957 0.920
    男性 −1.844 0.530 12.119 1 < 0.001 0.158 0.056 0.447
    Cdx-2-G等位基因 −1.201 0.568 4.464 1 0.035 0.301 0.099 0.917
    TPO-Ab 0.494 0.246 4.027 1 0.045 1.639 1.012 2.655
    TG-Ab 0.576 0.215 7.183 1 0.007 1.779 1.167 2.711
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-06-06
  • 网络出版日期:  2021-08-04
  • 刊出日期:  2021-08-04

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