基于Oncomine 数据库研究PDE4D基因在卵巢癌中的表达及血根碱的调控作用

邓玥; 邹丹; TounaAbdelkerim Barh; 杨丽华

doi:10.12259/j.issn.2095-610X.S20220303

基于Oncomine 数据库研究PDE4D基因在卵巢癌中的表达及血根碱的调控作用

doi: 10.12259/j.issn.2095-610X.S20220303

昆明医科大学第二附属医院妇科，云南昆明　650101

基金项目: 国家自然科学基金资助项目（81960469）；云南省“万人计划”名医专项（YNWR-MY-2019-037）；昆明医科大学卵巢癌临床及基础研究科技创新团队（CXTD202008）；昆明医科大学研究生创新基金资助项目（2021S235）

详细信息

作者简介:
邓玥（1997～），女，云南丽江人，在读硕士研究生，主要从事妇科肿瘤及妇科内分泌临床工作

通讯作者:
杨丽华，E-mail：lihuazhang33@sina.com

中图分类号: R3;R71
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出版历程
- 收稿日期: 2022-01-02
- 网络出版日期: 2022-02-18
- 刊出日期: 2022-03-22

Expression of PDE4D Gene in Ovarian Cancer Based on Oncomine and the Regulation of Sanguinarine

Dept. of Gynecology，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

摘要

摘要: 目的通过深度挖掘Oncomine数据库中PDE4D在卵巢癌的研究数据，分析PDE4D基因在卵巢癌中的表达变化，并进行细胞实验研究血根碱对卵巢癌细胞中PDE4D基因表达的影响。方法 PharmMapper数据库查询与血根碱药效基团匹配的作用靶点，收集Oncomine数据库 PDE4D在卵巢癌中的研究数据，进一步分析PDE4D在卵巢癌中的表达变化。将卵巢癌SKOV3和A2780细胞分成对照组和血根碱组，CCK8和RT-qPCR法分别检测血根碱对卵巢癌A2780、SKOV3细胞增殖及PDE4D mRNA表达的影响。结果 PDE4D是与血根碱药效基团匹配的作用靶点。Oncomine数据库中共查询到6项正常卵巢组织和卵巢癌组织PDE4D基因的研究，且PDE4D在不同类型的卵巢癌组织中均呈高表达（P < 0.05）。细胞实验表明，与对照组相比，血根碱组卵巢癌A2780、SKOV3细胞增殖被明显抑制，PDE4D mRNA表达降低，差异均有统计学意义（P < 0.05）。结论卵巢癌组织中PDE4D基因呈高表达，血根碱抑制卵巢癌细胞生长可能与下调PDE4D表达相关。
- 卵巢癌 /
- PDE4D /
- Oncomine数据库 /
- 血根碱
Abstract: Objective To investigate the expression and clinical significance of PDE4D in ovarian cancer by excavating gene information in Oncomine and explore the effect of sanguinarine on PDE4D by cells experiment. Methods PharmMapper was used to find the targets matched with the pharmacodynamic groups of sanguinarine. The gene information about PDE4D in ovarian cancer research was collected in Oncomine, and the expression level was analyzed. The A2780 and SKOV3 ovarian carcinoma cells were divided into control group and sanguinarine group. CCK8 and RT-qPCR were used to detect the effects of sanguinarine on the proliferation and PDE4D mRNA expression of A2780 and SKOV3 cells. Results PDE4D was the target matched with the pharmacodynamic group of sanguinarine. There were 6 studies referred to PDE4D in ovarian cancer and normal sample collected in the Oncomine. The expression of PDE4D in tumor tissues was significantly higher than that in normal （P < 0.05）. The cell experiments showed that compared with the control group, the proliferation of A2780 and SKOV3 cells was significantly inhibited and the expression of PDE4D mRNA was decreased in the sanguinarine group, with statistical significance （P < 0.05）. Conclusions PDE4D gene may play a role in the occurrence and development of ovarian cancer, and sanguinarine can inhibit the growth of ovarian cancer cells, which may be related to the down-regulation of PDE4D expression.
- Ovarian cancer /
- PDE4D /
- Oncomine /
- Sanguinarine

HTML全文

图 1 Oncomine数据库中PDE4D在卵巢癌中的表达

Figure 1. Expression of PDE4D in ovarian cancer in Oncomine

下载: 全尺寸图片幻灯片

图 2 Oncomine数据库中PDE4D在不同卵巢癌芯片中的表达

Figure 2. Expression of PDE4D in different ovarian cancer studies in Oncomine

下载: 全尺寸图片幻灯片

图 3 血根碱对卵巢癌A2780、SKOV3细胞增殖的影响

A：对照组A2780细胞（×100）；B：血根碱组A2780细胞（×100）；C：对照组SKOV3细胞（×100）；D：血根碱组SKOV3细胞（×100）；E：CCK8法检测的细胞存活率。与对照组比较，^**P < 0.01；^***P < 0.001。

Figure 3. Effect of Sanguinarine on proliferation of A2780 and SKOV3 cells

下载: 全尺寸图片幻灯片

图 4 血根碱对卵巢癌A2780、SKOV3细胞PDE4D mRNA表达的影响

与对照组比较，^*P < 0.05，^**P < 0.01。

Figure 4. Effect of Sanguinarine on PDE4D mRNA expression of A2780 and SKOV3 cells

下载: 全尺寸图片幻灯片

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