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过表达三结构域蛋白48调控p-ERK1/2抑制胶质瘤生长的作用机制

姜右川 余妍 赵国 李世存 丁鹏

姜右川, 余妍, 赵国, 李世存, 丁鹏. 过表达三结构域蛋白48调控p-ERK1/2抑制胶质瘤生长的作用机制[J]. 昆明医科大学学报, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505
引用本文: 姜右川, 余妍, 赵国, 李世存, 丁鹏. 过表达三结构域蛋白48调控p-ERK1/2抑制胶质瘤生长的作用机制[J]. 昆明医科大学学报, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505
Youchuan JIANG, Yan YU, Guo ZHAO, Shicun LI, Peng DING. Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth[J]. Journal of Kunming Medical University, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505
Citation: Youchuan JIANG, Yan YU, Guo ZHAO, Shicun LI, Peng DING. Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth[J]. Journal of Kunming Medical University, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505

过表达三结构域蛋白48调控p-ERK1/2抑制胶质瘤生长的作用机制

doi: 10.12259/j.issn.2095-610X.S20240505
基金项目: 云南省科技厅-昆明医科大学应用基础研究联合专项重点基金资助项目(202001AY070001-160);云南省卫生高层次人才专项基金资助项目(L-2019020)
详细信息
    作者简介:

    姜右川(1996~),男,山东临沂人,在读硕士研究生,主要从事胶质瘤研究工作

    余妍与姜右川对本文有同等贡献

    通讯作者:

    丁鹏,E-mail:pengdwfmc@163.com

  • 中图分类号: R739.41

Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth

  • 摘要:   目的  探究过表达三结构域蛋白48(tripartite motif protein,TRIM)对胶质瘤生长的影响及其相关机制。  方法  将12只裸鼠随机均分为2组,分别接种过表达TRIM48的U87胶质瘤稳转株(oeTRIM48组)及其对照细胞株(Vector组)。接种后每3 d测定肿瘤体积,4周后取出肿瘤组织并记录瘤重。肿瘤组织做HE染色,并通过免疫荧光法检测Ki-67的表达,使用Western blot和免疫组化分别检测裸鼠肿瘤和人胶质瘤组织芯片中的TRIM48、ERK1/2和p-ERK1/2水平。  结果  oeTRIM48组裸鼠肿瘤体积、重量比Vector组裸鼠明显降低(P < 0.0001);HE染色结果显示oeTRIM48组细胞核减小、核分裂象减少;Ki-67阳性区域显著降低(P < 0.0001),而且oeTRIM48组p-ERK1/2蛋白水平比Vector组显著降低(P < 0.01)。组织芯片免疫组化显示,TRIM48和p-ERK1/2在癌旁组织分别呈高表达和低表达,在肿瘤组织则相反。  结论  过表达TRIM48能够抑制胶质瘤生长、增殖,其作用机制可能与ERK1/2信号通路有关。
  • 图  1  过表达TRIM48对胶质瘤生长的影响

    A:肿瘤组织重量;B:肿瘤组织体积;C:肿瘤组织HE染色(×200);n=6; ****P < 0.0001。

    Figure  1.  Effect of TRIM48 overexpression on glioma growth

    图  2  过表达TRIM48影响胶质瘤组织增殖活性

    n=5,****P < 0.0001。

    Figure  2.  Overexpression of TRIM48 affects the proliferation activity of glioma tissues

    图  3  过表达TRIM48对ERK1/2通路的调控

    A:qRT-PCR和Western blot检测转染结果;B:Western blot检测过表达TRIM48对ERK1/2信号通路激活情况。n=6; ns:差异无统计学意义;*P < 0.05;**P < 0.01;****P < 0.0001。

    Figure  3.  Regulation of ERK1/2 pathway by overexpression of TRIM48

    图  4  胶质瘤患者组织芯片中TRIM48与ERK1/2信号通路之间的关系(×200)

    A:通过免疫组化检测组织芯片中胶质瘤患者肿瘤组织及匹配癌旁组织TRIM48与p-ERK1/2表达情况;B:p-ERK1/2在肿瘤组织与癌旁组织中的阳性区域;C:TRIM48在肿瘤组织和癌旁组织中的阳性区域;n=6;****P < 0.0001。

    Figure  4.  Direct relationship between TRIM48 and ERK1/2 signaling pathway in glioma tissue microarray (×200)

    表  1  PCR引物

    Table  1.   PCR primers

    引物名称目的基因序列(5'→3')产物大小(bp)
    Forward primer 1TRIM48AGCACCGGTATCACAGACAC162
    Reverse primer 1TRIM48TGTCTCCAAAAGCCTTCCAGTG162
    Forward primer 2β-actinAGGATTCCTATGTGGGCGAC273
    Reverse primer 2β-actinATAGCACAGCCTGGATAGCAA273
    下载: 导出CSV
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  • 收稿日期:  2024-01-15
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