miR-146a基因多态性与宫颈上皮内瘤变的相关性

师雨晗; 柴江红; 许金美; 林牧; 姚宇峰; 何凤权; 严志凌

miR-146a基因多态性与宫颈上皮内瘤变的相关性

师雨晗¹,
柴江红²,
许金美³,
林牧⁴,
姚宇峰¹,
何凤权^5, ,,
严志凌^{2, 3, ,}

1.
中国医学科学院 & 北京协和医学院医学生物学研究所，云南昆明　650118
2.
元谋县第一人民医院妇产科，云南元谋　651300
3.
昆明医科大学第三附属医院妇科，云南昆明　650118
4.
云南省保山市第二人民医院检验科，云南保山　678000
5.
红河州妇幼保健院妇产科，云南红河　661199

基金项目: 云南省基础研究计划基金（202201AY070001-139）；保山市科技计划项目-2023年医学研究联合专项基金（2023bskjylms016）

详细信息

作者简介:
师雨晗（2002～），女，云南昆明人，在读硕士研究生，主要从事肿瘤的免疫遗传学工作

通讯作者:
何凤权，E-mail：569451434@qq.com

严志凌，E-mail：yanzhiling2021@126.com

中图分类号: R737.33
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- 文章访问数: 45
- HTML全文浏览量: 29
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2024-09-12
- 网络出版日期: 2025-01-04

The Association between miR-146a Gene Polymorphism and Cervical Intraepithelial Neoplasia

Yuhan SHI¹,
Jianghong CHAI²,
Jinmei XU³,
Mu LIN⁴,
Yufeng YAO¹,
Fengquan HE^{5
, ,},
Zhiling YAN^{2, 3
, ,}

1.
Institute of Medical Biology，Chinese Academy of Medical Sciences & Peking Union Medical College，Kunming Yunnan 650118
2.
Department of Obstetrics and Gynecology，The First People’s Hospital of Yuanmou County，Yuanmou Yunnan 651300
3.
Department of Gynaecologic，The Third Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118
4.
Department of Laboratory Medicine，Baoshan Second People’s Hospital，Baoshan Yunnan 678000
5.
Department of Obstetrics and Gynecology，Maternal and Child Health Hospital of Honghe Prefecture，Honghe Yunnan 661199，China

摘要

摘要: 目的探究miR-146a基因的单核苷酸多态性（single nucleotide polymorphisms，SNP）rs57095329、rs6864584与宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）的相关性。方法利用SPSS软件随机收集96例CIN患者作为CIN组，225名健康个体作为对照组，采用TaqMan探针法对以上SNP位点进行基因分型，分析其与CIN的相关性。结果 rs57095329位点的等位基因和基因型分布相对于对照组差异具有统计学意义，CIN组中等位基因A的频率显著低于对照组（P < 0.001；OR = 0.48，95%CI：0.32～0.70）；在显性模式下，携带G等位基因（A/G-G/G）的个体CIN发生风险显著升高（P < 0.001；OR = 2.67，95% CI：1.64～4.37）。但rs6864584位点与CIN的发生风险无相关性。结论 miR-146a基因rs57095329位点的A等位基因可能是CIN的保护性因素。
- MicroRNAs /
- 宫颈上皮内瘤变 /
- 单核苷酸多态性
Abstract: Objective To investigate the association between single nucleotide polymorphisms （SNP） rs57095329 and rs6864584 of miR-146a gene and cervical intraepithelial neoplasia （CIN） . Methods A total of 96 patients diagnosed with CIN were randomly collected as the CIN group, and 225 healthy individuals examined during the same period were selected as the control group using SPSS software. Genotyping of the above SNP loci was performed using the TaqMan probe method. and their correlation with CIN was analyzed. Results The allele and genotype distribution of rs57095329 showed a statistically significant differences compared to the control group. with the frequency of the allele A in the CIN group significantly lower than that in the control group （P < 0.001; OR = 0.48, 95%CI: 0.32～0.70）. In the dominant model, individuals carrying the G allele （A/G-G/G） had a significantly increased risk of CIN （P < 0.001; OR = 2.67, 95%CI: 1.64～4.37）. In contrast, no correlation was found between the rs6864584 and the risk of CIN. Conclusion The A allele of the miR-146a gene at the rs57095329 locus may be a protective factor for CIN.
- MicroRNAs /
- Cervical intraepithelial neoplasia /
- Single nucleotide polymorphisms

HTML全文

图 1 rs57095329参与的DNA特征和调控元件

Figure 1. DNA features and regulatory elements involved in rs57095329

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图 2 rs57095329对SPI1表达的eQTL分析

Figure 2. eQTL analysis of rs57095329 on SPI1 expression

下载: 全尺寸图片幻灯片

表 1 SNP位点的等位基因和基因型在对照组和CIN组中的分布特征[n （%）]

Table 1. Distribution characteristics of allele and genotype at SNP loci in the control group and CIN group [n （%）]

SNPs	等位基因/基因型	对照组	CIN组	HWE		对照组 vs CIN组
SNPs	等位基因/基因型	对照组	CIN组	χ²	P	χ²	P	OR （95%CI）
rs57095329	A	370（82.2）	132（68.8）	0.742	0.389	14.325	< 0.001^*	0.48（0.32～0.70）
	G	80（17.8）	60（31.2）
	A/A	154（68.4）	43（44.8）			15.888	< 0.001^*
	A/G	62（27.6）	46（47.9）
	G/G	9（4.0）	7（7.3）
rs6864584	C	38（8.4）	13（6.8）	0.116	0.733	0.515	0.473	0.79（0.41～1.51）
	T	412（91.6）	179（93.2）
	C/C	2（0.9）	0（0.0）			1.014	0.602
	C/T	34（15.1）	13（13.5）
	T/T	189（84.0）	83（86.5）
^*P < 0.025。

下载: 导出CSV

表 2 SNP位点在对照组和CIN中的遗传模式分析[n （%）]

Table 2. Genetic model analysis of SNP loci in the control and CIN groups [n （%）]

SNPs	模型	基因型	对照组	CIN组	对照组 vs CIN组
SNPs	模型	基因型	对照组	CIN组	χ²	P	OR （95%CI）
rs57095329	共显性	A/A	154（68.4）	43 （44.8）	14.555	< 0.001^*	1.00
		A/G	62 （27.6）	46 （47.9）			2.66（1.60～4.42）
		G/G	9 （4.0）	7 （7.3）			2.79（0.98～7.91）
	显性	A/A	154（68.4）	43 （44.8）	15.879	< 0.001^*	1.00
		A/G-G/G	71 （31.6）	53 （55.2）			2.67（1.64～4.37）
	隐性	A/A-A/G	216（96.0）	89 （92.7）	1.539	0.230	1.00
		G/G	9 （4.0）	7 （7.3）			1.89（0.68～5.23）
	超显性	A/A-G/G	163（72.4）	50 （52.1）	12.496	< 0.001^*	1.00
		A/G	62 （27.6）	46 （47.9）			2.42（1.47～3.97）
	逻辑累加	---	---	---	17.055	< 0.001^*	2.12（1.42～3.16）
rs6864584	共显性	T/T	189（84.0）	83 （86.5）	0.155	0.450	1.00
		T/C	34 （15.1）	13 （13.5）			0.87（0.44～1.73）
		C/C	2 （0.9）	0 （0.0）			---
	显性	T/T	189（84.0）	83 （86.5）	0.314	0.570	1.00
		T/C-C/C	36 （16.0）	13 （13.5）			0.82（0.41～1.63）
	隐性	T/T-T/C	223（99.1）	96（100.0）	0.859	0.230	1.00
		C/C	2 （0.9）	0 （0.0）			---
	超显性	T/T-C/C	191（84.9）	83 （86.5）	0.133	0.710	1.00
		T/C	34 （15.1）	13 （13.5）			0.88（0.44～1.75）
	逻辑累加	---	---	---	0.519	0.470	0.79（0.41～1.51）
^*P < 0.05。

下载: 导出CSV

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